Microscopy (Bio 1 Lab)
How is focus achieved in a microscope?
Adjusting the distance between the specimen and the objective lens
What are microscopes used to often study?
Bacteria, tissues, cells
How is higher magnification achieved?
By moving the objective lens (may have to move focus)
Which factor affecting image quality is altered by light source?
Contrast
A microscope is a necessary tool in what fields of study?
Cytology, histology, pathology, microbiology
The lenses of a brightfield microscope are responsible for what of the object you are viewing?
Magnification
What image quality factor is usually expressed in a number such as 40x?
Magnification
What is resolution affected by?
Magnification
What are the three factors that determine the quality of a microscopic image?
Magnification, resolution, contrast
How do you calculate total magnification?
Multiply magnification of ocular lens(10x) with magnification of objective lens
What is a non motile specimen?
Not able to move
What lenses further magnify the image produced by the objective lens?
Ocular
What is total magnification?
The overall enlargement of the image of a specimen
Without making any other adjustments what can be increased by focusing properly?
The resolution
What are advantages to using a wet mount slide preparation?
The specimen could be viewed moving. The specimen behavior can be viewed. The specimen may be alive.
What is the goal of microscopy?
To create a magnified image of objects too small to be seen with the eye alone.
What is the fine adjustment knob(in the center) used for?
To fine tune the focus
The distance between the ocular lenses is used for what?
To fit the space between your eyes
What is the course focus ring used for?
To locate the focus plane
The stage adjustment knobs are used for what?
To move the stage left, right(lower), backwards, forwards(upper)
What do you increase the magnification for?
To study the region in detail
Which of the following items will you need to make a wet mount?
Transfer pipette, clean slide, tweezers, cover slip
Why should the fine focus knob typically be used with the and objective lenses?
Using the coarse knob would result in a large change in focus while only a small change is needed
What must be visually studied using electron microscopy?
Viruses
What is cytology?
study of cells
What is pathology?
study of disease
What is histology?
study of tissues
What is magnification?
the apparent increase in size of an object
What is the total magnification of a specimen using the 40x objective?
400x ocular lens x magnifying lens 40 x 10 = 400
The total magnification achieved using a 10x objective lens with a 10x eyepiece lens is 20x
False; 100x
What is different about the 100x lens?
Is is an oil objective
What is the stage clip used for?
It is used to hold the slide in place.
What is contrast affected by?
Light intensity and staining
Which objective lens provides the highest total magnification?
Oil immersion
Which objective lens provides the least total magnification?
Scan
What is microbiology?
Studies singular and multicellular microscopic organisms
What is resolution?
The ability to distinguish fine detail
What is contrast?
The ability to distinguish objects such as cells, from the background
What is the field of view?
The amount of the slide you are able to see through the eyepiece
What is the advantage of starting with the 4X objective before moving to the and then the 40x objective?
The field of view is larger and it is easier to focus
How do the coarse and fine focus knobs work on a brightfield microscope?
The focus knobs move the stage up and down
What do brightfield microscopes use to view the specimen?
a combination of glass lenses and light to view the specimen
What is a motile specimen?
able to move