Phlebotomy

Place the following steps in the correct sequence when preparing to perform a venipuncture.

-identify the patient and explain the procedure -wash hands and put on gloves -apply tourniquet, palpate the antecubital space of the arm -locate an acceptable vein

The appropriate needle gauge to use for most venipuncture collections is

21. Rationale The 21 gauge needle is long enough and thin enough to access most veins and the internal diameter (bore size) is wide enough to permit blood collection with little concern about mechanical hemolysis when using evacuated tubes. Butterfly collection sets (wing sets) typically use needles of a higher gauge, and blood donation centers typically use needles of a lower gauge. Remember, the higher the gauge, the smaller the bore (opening).

After selecting the appropriate location to collect a blood sample, how many inches above that location should the phlebotomist fasten the tourniquet?

3 to 4 inches Rationale Tourniquets are used to make it easier to locate veins by causing them to become distended and easier to palpate.

The medical assistant is educating the patient on fasting diet requirements prior to a glucose tolerance test. Which of the following is the minimum time frame the patient must remain NPO before the start of the test?

8-12 hours Rationale To ensure accuracy of the blood test results, the patient must remain NPO for 8-12 hours prior to the test. Gum chewing, eating mints, and/or smoking are not allowed. Sips of water may be permitted depending on the provider NPO policy.

Which patient description is correctly matched with the appropriate site selection? A. A 53-year old female that has had a double mastectomy has a specimen collected on the palmar side of the wrist. B. A two-day old male has a specimen collected from central section of the plantar surface of the heel. C. A two-year old female has a specimen collected from the palmar surface of the distal phalanx of the fifth finger. D. A 53-year old male with a central line has a specimen collected from the dorsal side of the hand.

A 53-year old male with a central line has a specimen collected from the dorsal side of the hand. Rationale Patients with central lines would be candidates for specimen collection from dorsal side of the hand. Venous samples are not collected from the palmar side of the wrist; veins are accessible from the dorsal side.

Which of the following actions should the medical assistant take to make a vein more prominent when attempting to select a venipuncture site?

Apply a warm compress to the area for 5 minutes.

An infant is brought to the clinic for a CBC. After performing a heelstick, not enough blood is collected to perform the test. Which of the following actions should the medical assistant take?

Apply a warm compress. Rationale The heat from a warm compress will encourage capillary vasodilation resulting in increased blood flow.

Which of the following is the proper way to identify a patient prior to performing a venipuncture?

Ask the patient to state his/her full name and DOB and compare to the chart.

Which of the following actions by the medical assistant demonstrates safe use of a centrifuge?

Balance the tube load to prevent breakage. Rationale It is important to properly balance a centrifuge, equally distributing the samples. An imbalance of weight could cause the centrifuge core to break while spinning, and/or cause damage to the rotors, brushes, or other components. The shaking of an imbalanced centrifuge could also lead to tube breakage. Tubes within a centrifuge should be capped at all times (to contain all samples and aerosols). It is not necessary to stop the centrifuge to make sure samples are separating or to monitor function.

What is the laboratory abbreviation for calcium?

Ca Rationale Ca is the scientific abbreviation for calcium. The laboratory uses scientific abbreviations from the Periodic Table of Elements. CA = cancer, carcinoma. Calorie = cal. CAL can indicate many things, but is not the general abbreviation for calcium.

Prior to any type of inpatient specimen collection, a phlebotomist must correctly verify their patient's identity by using which two-step procedure?

Check ID bracelet, and ask patient to verbally confirm their identity.

The phlebotomist needs to draw routine labs. The skin preparation for this procedure would be.

Cleanse the site by using a circular motion from center to periphery Rationale Phlebotomists cleanse the selected venipuncture site before routine lab draws.

The medical assistant is collecting a sample on a 2 year old outpatient by finger stick. Following the collection, the medical assistant holds firm pressure on the site until bleeding has stopped. How should the medical assistant proceed?

Do not bandage the site. Rationale If the child has shown evidence of wanting to touch everything in sight, the phlebotomist should not add a bandage to the list of things within his reach as a 2 year old could easily decide to put it in his mouth

Patient has a DVT in left arm and the patient is also on blood thinners, where would be the best site for drawing blood?

Draw right arm and hold for 5 minutes Rationale A patient with a deep vein thrombosis (DVT) in the left arm requires special precautions during a phlebotomy procedure. Blood thinners are a common treatment for DVT.

Identify the CLSI recommended micro-collection order of draw for a BMP, CBC & Bilirubin.

EDTA, Heparin, Serum Separator Rationale The recommended Order of Draw for capillary blood collection is different from blood specimens drawn by venipuncture. CLSI recommends the following order of draw for skin puncture: Blood gases, EDTA tubes, Other additive tubes, then Serum tubes.

When performing a CBC on a six year old, the parent insists upon using an anesthetic at the venipuncture site. Which would be correct?

EMLA Rationale EMLA is the topical anesthetic of choice for pediatric venipuncture. The other anesthetic choices in this question are not typically available to or used by phlebotomists. EMLA Cream (lidocaine 2.5% and prilocaine 2.5%) is an emulsion, with a mixture of lidocaine and prilocaine.

The physician orders a sedimentation rate for a patient recovering from an infection. This is indicated on the lab requisition as which of the following?

ESR Rationale The Erythrocyte Sedimentation Rate (ESR) is a common test to detect inflammation by measuring the rate at which red blood cells settle to the bottom of a tall, thin tube.

Since special precautions are needed for patients with Vascular Access Devices, which of the following devices are contraindicated(Anything (including a symptom or medical condition) that is a reason for a person to not receive a particular treatment or procedure because it may be harmful) for any type of blood draws?

Fistula Rationale The AV fistula requires the surgical connection of a vein and artery, typically in the forearm. It is predominately used for patients in renal failure who may need long term dialysis.

Which of the following actions should the medical assistant take to prevent hemolysis of a blood specimen in an EDTA tube?

Gently invert the tube after collecting the specimen.

Which of the following tests is drawn in an EDTA tube for the hematology department of the laboratory?

HGB Rationale HGB is an abbreviation for Hemoglobin, typically measured as part of a Complete Blood Count and collected in an evacuated tube containing Ethylenediaminetetraacetic acid (EDTA).

A patient's past history indicates episodes of syncope. Which of the following would be the best course of action the phlebotomist would perform to prevent complications of syncope?

Have the patient lie down on a hospital bed or exam table. Rationale Syncope is a temporary loss of consciousness and posture, commonly referred to as fainting, which is usually related to insufficient blood flow to the brain for any number of reasons.

Which of the following characteristics applies to veins

Have valves to prevent backflow of blood Rationale Veins have valves which maintain the unidirectional flow of blood, even against gravity. Veins are also the blood vessels which carry the blood from peripheral tissues towards heart.

After a phlebotomy draw, the phlebotomist realizes the tourniquet was on the patient's arm for an extensive length of time. Which of these is the most common complication?

Hemoconcentration Rationale Hemoconcentration is decrease in the volume of plasma in relation to the number of red blood cells. Prolonged tourniquet use impedes circulation and will cause this to happen, but will likely not cause hemolysis or a hematoma.

The medical assistant completes a venipuncture in the antecubital area without complications. Pressure is placed on the site following the collection. The patient is still bleeding after five minutes of continuous pressure by the medical assistant and there is bruising at the site. The medical assistant asks the patient if she is on anticoagulants. The patient indicates that she is, and states that this is normal for her. How should the medical assistant proceed?

Hold direct pressure until bleeding stops, apply a bandage, and notify nurse. Rationale It is the responsibility of the phlebotomist to ensure bleeding has stopped before leaving the patient.

During a venipuncture the patient has excessive bleeding and then states she forgot to mention her anticoagulant (Coumadin) therapy. Which of the following should the medical assistant do?

Hold pressure on the site for a minimum of five full minutes until bleeding stops. Rationale A patient on Coumadin therapy will have thin blood and the Medical Assistant will have to hold at least 5 minutes of continuous pressure to be sure a clot has formed at the puncture site. Coumadin is an anticoagulant, it thins the blood, so it takes longer to clot.

A phlebotomist is staffing the outpatient clinic at a remote site. There is a centrifuge at the site, but it is not functional. The courier will be arriving to pick-up all samples collected at 1300, and will deliver to the testing laboratory at 1500. A patient arrives at 1200 with orders for Complete Blood Count, Prothrombin Time, and Basic Metabolic Panel. Which tubes should the phlebotomist use for blood collection?

Lavender, Blue, Green, Gray

Which of the following Vacutainer tubes is routinely used for hematology testing?

Lavender-topped Rationale Hematology is a general term or branch of medicine that studies blood, the organs that form blood, and blood disorders and diseases. The most common hematology test is a complete blood count (a.k.a. CBC- collected in a lavender topped tube), often ordered with a cell differential.

A physician asked you to draw a INR on Mr. Jones which tube and additive would you choose

Light Blue, Sodium Citrate

The laboratory requisition indicates that you are to collect a venous blood specimen for hematology CBC testing, serum chemistry testing, and coagulation studies. What is the proper order of collection for these tubes?

Light blue, red, lavender Rationale Light blue (coagulation studies), red (serum chemistry), then lavender (hematology CBC) tubes follows CLSI established order of draw.

Which of the following tube tops contain silica particles to enhance clotting and a thixotropic gel to assist in the separation of serum and blood cells?

Marbled red and grey Rationale Marbled red and gray top tubes contain silica particles enhance the clot formation of whole blood. When the sample is centrifuged, the gel will form a barrier between the serum and blood cells, with the serum portion on top. Lavender, green, and blue topped tubes all have anticoagulants that inhibit the clotting process.

Which of the following tests is required in all 50 states to screen newborns for metabolic disease?

PKU Rationale The government has mandated newborn screening for the purpose of early diagnosis and treatment of Phenylketonuria (PKU). It is an inherited disease that may lead to mental retardation if not detected. The specimen for the test is usually collected in the newborn nursery via heel stick between 48-72 hours of life.

Which of the following tests monitor anticoagulation therapy?

PT and PTT Rationale PT and PTT are coagulation tests (collected in blue top tubes). They are routinely collected to monitor a patient's anticoagulant drug therapy (to make sure that the dose the patient is taking provides the proper effect, but does not dangerously over-anticoagulate and introduce a critical risk of bleeding). In short, these tests monitor a patient's blood clotting time.

Name the two most used patient identifiers.

Patient's name/date of birth Rationale Name and DOB are most often used as positive patient identifiers as they are easily documented and work for inpatients or outpatients.

A phlebotomist inserts a needle into a patient's vein and notices there is no blood flow into the tube. The needle is positioned against the wall of the vein. How should the phlebotomist proceed?

Relax the needle's angle slightly, and insert new tube Rationale If the needle is believed to be positioned against the vein wall, sometimes relaxing the needle's angle slightly will bring blood into the blood collection tube. In case the tube's vacuum was compromised, using a new tube should provide blood flow.

When performing a venipuncture, sudden swelling is observed at the insertion site. Which of the following actions should the medical assistant take?

Release the tourniquet, remove the needle, and immediately apply pressure at the site. Rationale The medical assistant should release the tourniquet, remove the needle, and immediately apply pressure to the site. The sudden swelling is an indication that the needle has traumatized or penetrated through both sides of the vein. This is also referred to as a "blown" vein.

During a blood draw, a patient begins complaining of discomfort and tingling, at which time the phlebotomist notices bruising and discoloration at the site. How should the phlebotomist proceed?

Release the tourniquet, withdraw the needle, and apply pressure. Rationale The correct response is to release the tourniquet, withdraw the needle, and apply pressure to avoid any further damage or pain at the venipuncture site. The tourniquet MUST be released before the needle is withdrawn.

Using the guidelines for any method of venipuncture, which of the following actions should the medical assistant perform directly after confirming patient identity?

Review the requirements for collecting and handling the blood specimen as ordered by the physician Rationale To ensure accuracy and quality, it is important to anticipate your needs before beginning the actual venipuncture (i.e. tube color/size, minimum acceptable blood volume, whether or not the specimen needs to be placed on ice).

Which of the listed tubes can contain ethylenediamine tetraacetic acid?

Royal blue Rationale Royal blue blood Collection tubes may be purchased with EDTA, sodium heparin, or no additive at all.

The phlebotomist is drawing plain red, lavender, and blue top tubes. Upon completion of the blood draw, the phlebotomist noticed that an order that requires a SST has been missed. What would be the next action?

Share the plain red top specimen for the SST test.

The medical assistant is collecting four tubes of blood on a patient who begins wheezing. She asks the patient if he is okay, he states that he has asthma. The medical assistant is in the process of switching to the fourth tube when the patient faints in the phlebotomy chair. Which of the following steps should the medical assistant take?

Stop the collection, apply pressure to site, call for help, and assist the patient. Rationale When a patient faints, the most important consideration is patient safety.

A medical assistant has active symptoms of the common cold. Under which of the following conditions may he perform venipuncture?

The medical assistant may perform venipuncture provided he is wearing a mask and afebrile. Rationale It is not uncommon for people to be able to work with the common cold. However, it is important for medical personnel to use good judgment and not transmit diseases to their patients.

When performing a skin puncture on an infant what is the preferred site?

The plantar surface of the heel Rationale The heel of an infant is the recommended skin puncture site. Avoid puncturing the bone by targeting the plantar surface.

After centrifuging a blood specimen, the medical assistant notes the serum has a reddish appearance. Which of the following has occurred?

The specimen is hemolyzed. Rationale Normal serum should be amber-colored. If it's reddish in appearance, the specimen is hemolyzed. The reddish color is caused by broken red blood cells, not neutrophils (which are white blood cells). The buffy coat is the layer of white blood cells and platelets that forms in whole blood between the plasma and the red blood cells. This question refers to serum, which is the liquid layer of clotted blood (the white blood cells/platelets would be contained within the clot).

Which of the following statements best describes the reason antecubital veins are most desirable to use when performing venipuncture?

The tissue surrounding these veins is less sensitive, making the procedure less painful. Rationale Of the three veins in the antecubital area acceptable for venipuncture, the median cubital vein (in the middle) is the vein of choice for four reasons: 1) it's more stationary; 2) puncturing it is less painful to the patient; 3) it's usually closer to the surface of the skin; and 4) it isn't nestled among nerves or arteries.

When a complete metabolic profile (CMP) and complete blood count (CBC) are ordered at the same time, why should the CMP be drawn first?

Tubes without additives should be drawn before tubes with additives. Rationale A blood specimen will naturally clot (yielding a serum component) when collected in a tube without an additive to anticoagulate it and keep it whole blood (which has a plasma component).

A phlebotomist is asked to collect a CBC, PT and Chem 7 on a patient that requires a hand stick with a butterfly needle into evacuated tubes. The correct order of draw for this collection would be

Two Light Blue Top Tubes, Green Top Tube, Lavender Top Tube Rationale The correct order of draw would be: two light blue top tubes (one waste and one for PT which is a coagulation test), green top tube (for the Chem 7), and the lavender top tube (for the CBC).

When inserting a needle during venipuncture, the bevel should be in which of the following positions in relation to the vein?

Up

After several minutes of searching the medical assistant can only palpate a small vein. The patient is prepared and the venipuncture is performed. The blood is filling the tube, but slows and then stops even though the patient and medical assistant remained still. Which of the following actions should the medical assistant take?

Use a smaller tube because the vein has collapsed. Rationale Since blood was once filling the tube, the needle was once in the vein. Since the phlebotomist knew this was a small vein at palpation, the fragility of the vein may have caused the vein to collapse if the vacuum of a regular size collection tube was too strong. Putting a smaller tube on the multi-sample needle would decrease the force of the vacuum pulling blood from the vein. If blood flowed easily again, problem is solved!

After a patient's skin is punctured to obtain a blood glucose level, what should be the phlebotomist's next step?

Use a sterile gauze pad to remove the first drop. Rationale It's important to wipe off the first drop of blood in any capillary collection, as this drop may include sufficient tissue fluid to influence test results

Filling evacuated tubes to the indicated level results in the correct ratio of

additive to blood

When performing a venipuncture on an infant, the amount of blood taken from the infant should be monitored to avoid

anemia Rationale The removal of just 10 mL of blood from an infant can result in a 10% loss of total blood volume and lead to iatrogenic anemia caused by blood collection. This is most serious in the first week of life.

Which of the following types of additives is present in an EDTA tube?

anticoagulant Rationale An EDTA tube contains the anticoagulant sodium ethylenediamine tetra-acetic acid, which prevents clotting.

EDTA is an

anticoagulant. Rationale Ethylenediaminetetraacetic acid (EDTA) is a chelating agent in lavender top blood collection tubes that binds calcium, an essential component for human blood coagulation.

To help prevent a hematoma at the site after drawing blood, which of the following actions does the medical assistant take?

apply direct pressure with cotton gauze Rationale To promote clotting and help prevent the leakage of blood outside of the vein (hematoma or bruise), the medical assistant should apply direct pressure with cotton gauze.

Capillary blood specimens are typically collected on patients that

are extremely obese. Rationale Capillary blood samples are advisable on patients when veins are not palpable, which includes patients who are morbidly obese, among others. A phlebotomist should never just "hope for the best" if a vein cannot be located.

An entry level medical assistant goes to collect blood from a 70-year-old woman with diabetes who had a mastectomy of the left breast. The medical assistant notices a PICC line in the patient's right arm. For this specimen collection, the medical assistant should

ask a nurse to collect the specimen. Rationale This scenario is a scope of practice example. The medical assistant should ask a nurse to draw the sample.

At what time should a test tube be labeled with the patient's identification?

before leaving the patient's room, after drawing the blood (1) positively identifying the patient (2) collecting the patient's blood into tubes (3) labeling the tubes immediately afterward. Any change in this sequence or any significant interruption between steps has been linked to significantly increased chances for error.

Which of the following specimens is most susceptible to cross contamination and should always be drawn first?

blood culture Rationale Since blood cultures test for sepsis (infection in the blood stream), the skin should be specially prepared to avoid contaminating the specimen during the collection process with normal skin bacteria.

The medical assistant has an order to collect a CBC with diff, PT, a basic metabolic panel (BMP), and blood cultures. Place the collection tubes in the correct order of draw.

blood culture bottles, light blue, red, lavender

Which of the following is an appropriate alternative to using a rubber tourniquet when attempting a venipuncture on a known patient who is difficult to obtain a sample from?

blood pressure cuff Rationale The only acceptable choice among the listed options is the blood pressure cuff. Occlusion pressure may be adjusted as needed as an alternative to ensure circulation.

The physician has ordered a prothrombin time (PT) and complete blood count (CBC) for an adult patient with a diagnosis of thrombophlebitis. The medical assistant should collect the evacuated tubes in which of the following orders?

blue, lavender

While performing a venipuncture in the antecubital space, the patient verbalizes an extreme amount of pain and the blood in the collection tube is noted to be bright red. Which of the following sites is most likely to have been inadvertently punctured?

brachial artery Rationale The brachial artery is most likely punctured, because it is a large artery and the bright red bleeding and fast, pulsating movement indicates arterial blood flow and not venous.

A 2 week old infant has been brought to the lab for a repeat PKU/neonatal screening. Which of the following collection procedures is appropriate to collect the specimens?

capillary using heel puncture lancet

Which of the following forms is the medical assistant responsible for having the patient sign when a specimen is being collected for substance abuse screening for employment?

chain-of-custody Rationale A Chain-of-custody form is used to maintain a legal record of individuals who have had physical possession of the specimen. It prevents the possiblity of tampering

When performing a fingerstick on a patient, the phlebotomist must pay close attention to the extremity that has been compromised by

circulation, swelling, and infection. Rationale When performing fingersticks, dry skin and bug bites would not cause problems in collection or recovery from collection.

During processing, the centrifuge rotor lid should be kept

closed to maintain an airtight seal. Rationale To ensure safety, the lid of a centrifuge should remain closed, locked and sealed until the rotor reaches a complete stop.

The medical assistant collects blood for a test that requires a blood smear. What is the terminology that would indicate that the sample clumped (therefore requiring a redraw)?

coagulation Rationale Coagulation is a term that describes how blood clots (changes from a liquid to a clump or solid). Sometimes if the person performing a phlebotomy procedure fails to adequately mix the blood within a tube, the anticoagulant does not fully work and the sample will have clumps/clots.

While performing a venipuncture using an evacuated tube, a small amount of blood enters the tube and then stops. Which of the following conditions is likely to lead to this?

collapsing vein Rationale When blood ceases to flow during the venipuncture process, the likely culprit is a collapsing vein. The walls of the vein lose tension, stopping blood flow.

A medical assistant needs to draw a bilirubin on a jaundiced infant. The medical assistant should

collect the specimen in an amber tube to protect from light. Rationale Bilirubin is a photo-sensitive analyte (can degrade by as much as 50% with an hour of light exposure)

After a phlebotomist has successfully followed all the steps for blood collection, the patient informs the phlebotomist that he tends to bleed longer than the average person. What would be the next course of action for the phlebotomist?

confirm hemostasis and apply paper tape Rationale A proper phlebotomy procedure includes confirmation of hemostasis, regardless of whether or not a patient bleeds longer than normal. This definitely applies to this case when a patient admits a tendency to bleed longer than the average person.

After performing a venipuncture on a patient the medical assistant notices the patient is bending his elbow to hold the gauze in place. This action is contraindicated because bending the elbow is likely to

create a hematoma. Rationale If adequate pressure is not applied following a venipuncture, a hematoma can form via the transfer of blood from the vein into the surrounding tissue.

When collecting specimens for alanine aminotransferase (ALT) or aspartate aminotransferase (AST), avoid gray-topped sodium fluoride tubes because the anticoagulant

destroys many enzymes. Rationale The sodium fluoride in gray-topped tubes can destroy many enzymes. Since ALT and AST are enzyme tests, sodium fluoride should be avoided

Venipuncture on the foot is performed in which of the following locations?

dorsal pedis Rationale Basic anatomy, rationale not applicable.

Which of the following arteries provides direct blood flow to the foot?

dorsalis pedis Rationale The dorsalis pedis artery is located on the upper surface (dorsal region) of the foot, and provides direct blood flow to the foot. The popliteal artery is located behind the knee, the brachial artery is in the arm, and the femoral artery is in the thigh.

The medical assistant is asked to draw blood from a patient with an I.V. whose opposite, left arm is inaccessible. Drawing blood from which of the following sites would be appropriate without a physician's approval?

draw from the right arm distal to the I.V. site Rationale Collecting blood distal to the I.V. site prevents contamination of the sample by the intravenous solution

Which of the following additives protects serum from interacting with red blood cells after a specimen has been centrifuged?

gel separators Rationale Gel separators create a barrier between the serum and formed elements of the blood.

A test tube with which of the following colored stoppers has a glycolytic inhibitor?

gray Rationale A gray top blood collection tube contains powdered sodium fluoride and/or potassium oxalate, which will inhibit glycolysis.

Which of the following evacuated tubes should the medical assistant select for collection of electrolytes?

green-top Rationale A green top tube contains heparin. Green tops are used for collection of heparinized plasma or whole blood for special tests. Note: After the tube has been filled with blood, the tube is inverted several times to prevent coagulation. Note: Green top tubes can contain ammonium, lithium, and sodium in addition to the heparin. In this instance, lithium heparin would be the green-top of choice (and sodium heparin would NOT be used for an electrolyte test). Electrolytes can also be collected in a red-top tube.

The physician orders a hemoglobin level to be checked on a 2-month-old patient. Which of the following sites should the medical assistant use to obtain the specimen?

heel of the foot Rationale The heel of the foot is the recommended site to obtain a capillary sample on infants less than one year.

The medical assistant is instructed to perform a capillary stick for newborn screening. Which of the following collection devices should the medical assistant use?

heel stick lancet Rationale The heel stick lancet (e.g., Tenderfoot) is required for infants. If a heelstick in an infant is being performed, the phlebotomist should apply a warming device for approximately 3-5 minutes to the heel to increase blood flow to the area, which will facilitate the collection of the capillary specimen.

While performing a venipuncture, the medical assistant removes the filled tubes, but forgets to remove the tourniquet prior to needle withdrawal. Which of the following is the most likely result?

hematoma Rationale A hematoma is a large painful bruise at the puncture site, caused or created by blood seeping into the surrounding tissues, one of the causes is the delay in tourniquet release and pulling the vacuum tube out

Forceful shaking of blood in an evacuated collection tube will cause which of the following complications?

hemolysis Rationale Hemolysis is the breakdown/damage of blood cells and could end in an unusable specimen and costly re-draw for the patient.

A phlebotomist has received a requisition for a PT/aPTT to evaluate the patient for Von Willebrand's disease. Because of the patient's potential diagnosis, the phlebotomist should be sure to

hold pressure on the draw site until bleeding has stopped. Rationale Von Willebrand disease is an inherited condition that can cause extended or excessive bleeding. Those affected have deficient amounts or impaired von Willebrand factor, a protein important to the clotting process.

Which of the following steps is completed first in performing venipuncture?

identify the patient Rationale To prevent error and maintain a culture of safety, at least 2 identifiers (ex. full name and date of birth) must be verified prior to performing a procedure.

Which of the following should be avoided as a form of patient identification?

insurance number Rationale An insurance number is not a common means of positive patient identification (entire families can share the same insurance policy number). Patients must be positively identified for medical services

Which of the following tubes should the medical assistant select in collecting a blood sample for a Complete Blood Count (CBC)?

lavender top, ethylenediaminetetraacetic acid additive

The physician orders a prothrombin time. Which of the following tubes should be used to collect the specimen?

light blue Rationale A light blue top tube contains sodium citrate to prevent blood from clotting. This tube contains sodium citrate as an anticoagulant and is used when blood is drawn for coagulation studies. Note: It is imperative that the tube be completely filled. The ratio of blood to anticoagulant is critical for valid prothrombin time results. Immediately after the draw, the tube needs to be inverted 6 to 10 times to activate the anticoagulant.

A patient is at the lab for their weekly PT/INR. Which of the following tubes should the medical assistant collect for this test?

light blue top

Which of the following evacuated tubes should the medical assistant draw first?

light blue top Rationale CLSI lists the order of draw as follows: 1. Blood cultures 2. Coagulation tube (e.g. blue closure) 3. Serum tube with or without clot activator, with or without gel (e.g. red closure, gold shield,etc.) 4. Heparin tube with or without gel plasma separator (e.g. green closure) 5. EDTA tube with or without gel separator (e.g. lavender closure, pearl closure) 6. Glycolytic inhibitor (e.g. gray closure) Other tube additives affect coagulation tests. Collecting the light blue top tube (for coagulation testing) in this scenario prevents contamination of the specimen with tissue thromboplastin (which will adversely affect the test).

The patient was sent to the lab to have CBC (lavender), PT/PTT (light blue), CMP (green), and glucose (gray).

light blue, green, lavender, gray

The phlebotomist should send a specimen collected in a yellow stopper tube with SPS additive to which of the following laboratory departments?

microbiology Rationale Blood cultures are collected in yellow-top tubes with SPS (sodium polyanethole sulfonate), a constituent in blood culture media used to grow bacteria from patients suspected of bacteremia, i.e. bacteria in the blood. SPS prevents the patient's blood from killing any bacteria in the sample through the blood's own immune response to infection, allowing the bacteria to multiply so they can be identified. Additional antimicrobial susceptibility testing can help the physician in prescribing antibiotics.

A patient with an order for a blood draw presents with burns covering both arms. Which of the following is the medical assistant's best option for collecting the largest amount of blood for this patient?

microtainer tube Rationale A microtainer tube would be the best choice in this case. If a patient has burns covering both arms, a routine venipuncture from a brachial vein is not an option. The person collecting the blood will have the best chance at getting the highest volume of blood from a smaller vein using a microtainer tube (has less vacuum pressure than a regular sized tube). There are many ways to collect blood, and using a evacuated tube is generally the best method. But in case of a burn, the best procedure would be to do a capillary finger stick and then use a microtainer tube which works on the same principle. A PKU is collected on a Guthrie blot card and is only for newborn screening.

Which of the following items is more commonly found on a phlebotomy tray?

multi-sample needle

For which of the following patients is the use of an adhesive bandage contraindicated?

newborn with hyperbilirubinemia(jaundice) Rationale The person collecting a blood sample from a newborn with hyperbilirubinemia would not use an adhesive bandage post-stick. Bleeding would be stopped by direct pressure and no bandage would be utilized. It is not recommended to use a bandage on children under two years of age because it could introduce a choking hazard if the child works the bandage loose. Bandaging a newborn's foot is a controversial issue because of skin sensitivity and potential bandage aspiration.

The phlebotomist is drawing blood from a patient that is taking blood thinners. Which of the following is a necessary piece of additional equipment to have at the drawing station?

non-adhering bandage Rationale Non-adhering bandage material would be helpful for patients on anticoagulant therapy who may bleed more extensively than typical patients after venipuncture. It is necessary to contain the bleed with a bandage, but adhesive might bruise once removed from a patient who has anticoagulated blood.

Capillary blood specimen collections are contraindicated in patients with

peripheral edema. Rationale Patients with peripheral edema have a great deal of fluid in their tissue. Collection of capillary blood would provide a sample that is likely to be highly diluted with tissue fluid, affecting laboratory test results. Capillary testing would be a choice for the other patient conditions.

A phlebotomist has received a requisition to collect a blood culture. Using a butterfly, three milliliters of blood is collected. Into which of the following bottles is the proper dispensation of the specimen?

place all three mLs in the aerobic bottle Rationale Since the phlebotomist was only able to obtain three milliliters of blood, place all three mLs in the aerobic bottle. The aerobic bottle would be the first bottle to innoculate if there is limited sample. It is not advisable to split the innoculation of 3 total mLs into both bottles since it would not adequately innoculate either bottle. The fact that a butterfly was used in this instance indicates that the patient was probably a hard stick

Which of the following sites should the medical assistant use for newborn screening blood collection?

plantar surface of the heel Rationale A heel stick should be performed on the lateral or medial portions of the plantar surface of the heel-skin surface (left and right). Never stick the center area of heel, the arch, or the back of the heel.

A phlebotomist has received a requisition to collect a blood specimen on patient in isolation. When entering the patient's room, which should the phlebotomist do first?

put on the gown Rationale The phlebotomist should put on the gown, followed by the mask and gloves.

While performing a venipuncture, immediately before removing the needle, the medical assistant should

release the tourniquet. Rationale Right before the medical assistant removes the needle, he/she should release the tourniquet. If the tourniquet is left on for too long, blood flow to the extremity could be impaired.

The patient reports feeling faint, and slumps forward during a venipuncture. After calling for assistance, which of the following actions should the medical assistant take next?

remove the tourniquet Rationale The procedure should be discontinued immediately, with the initial step of releasing the tourniquet. The needle would also need to be removed, but the tourniquet should be removed first because it is causing pressure to the vein.

When performing venipuncture on a patient with a left mastectomy, which site should the medical assistant use?

right antecubital fossa Rationale Due to the mastectomy, avoid any left side sites. The right antecubital fossa is the best collection site. Blood samples should not be collected from the arm on any side a patient has had breast cancer surgery (a mastectomy or a lumpectomy with lymph node removal).

A centrifuge functions in which of the following ways?

rotates to separate components of a patient's blood

Which of the following is a likely complication from repeated phlebotomy procedures in the same area?

sclerosed veins Repeated punctures in a vein would cause hardening of the vessel tissue, known as sclerosis. Thrombosis refers to clot formation. Petechiae are tiny red or purple spots on the skin caused by broken capillaries. Varicose veins are caused by weakened valves and veins in the legs. One-way vein valves typically keep blood flowing from the legs up toward the heart, but when they don't work properly, blood collects in the legs and pressure builds. The veins become weak, large, and twisted.

Which of the following is the additive in the light blue top tube?

sodium citrate Rationale Lavender top tube = EDTA, Light blue top tube = sodium citrate, Green (dark) top tube = heparin, Gray top tube = potassium oxalate

A syringe is preferred over a vacuum collection device whe

the patient's veins are fragile. Rationale A needle and syringe would be chosen over a vacuum collection device when a patient has fragile veins. The vacuum pressure of evacuated tubes can collapse small or fragile veins such as those on the back of the hand. When many tests requiring multiple tubes are collected, vacuum devices are preferred. A capillary puncture would be indicated for a newborn screening PKU test since it is collected on filter paper.

88. Blood cultures are always drawn first because?

to avoid contamination of the cultures. Rationale When sepsis is suspected, the physician orders blood cultures. Specimen collection for blood cultures is more detailed than for routine venipuncture, thus adding collection time.

Which of the following actions is appropriate for the medical assistant to take if an evacuated tube does not even begin to fill during a specimen draw?

try another evacuated tube prior to withdrawing from the vein Rationale In this instance, it is important to maintain a closed system and avoid re-sticking the patient if possible.

In which of the following time frames should a tourniquet be routinely released on a patient while performing a blood draw?

within one minute