Unit 1 Test

Below you will find the steps involved in PCR, but they are shuffled. Put the number next to each step based on the order it is in the process. _____ DNA fragments are heated at high temperatures, separating the DNA into strands. _____ Process repeats, doubling the amount of DNA each time it cycles through. _____ Get cells from blood or saliva. _____ Mixture is cooled down. _____ DNA Polymerase makes the conplementary strands, creating two new DNA strands. _____ Primers attach to DNA strands. _____ Cells are loaded into PCR machine.

3, 7, 1, 6, 5, 4, 2

If a DNA molecule is 20% As, what % Cs is it?

30% C

Who would be responsible for performing an autopsy on a cadaver? A. Coroner B. Medical examiner C. EMT D. Toxicologist

B.

A person is found dead by a neighbor. What career is involved in notifying the police and calling an ambulance? A. 911 operator B. EMT C. Coroner D. Medical examiner

A.

If the sequence of a DNA strand is ATCGTTACGAAA, what would it's complimentary strand be? A. TAGCAATGCTTT B. ATCGTTACTAAA C. UAGCAAUCGUUU D. CGATGGCATCCC

A.

What characteristic separates DNA fragments during gel electrophoresis? A. Length of fragment B. Base pair sequence C. Shape of helix D. Color of stain

A.

What person is responsible for providing short-term care for people being transported to the hospital? A. EMT B. EKG technician C. CNA D. 911 Operator

A.

What are the legally defined manners of death?

Accident, suicide, homicide, natural, undetermined.

What are the three major fingerprint ridge patterns?

Arch, loop, whorl

Given the DNA strand GAATTCCTCGAG, what would be the complimentary strand to make the double stranded DNA? A. GAATTCCTCGAG B. CTTAAGGAGCTC C. AGGCCTTCTAGA D. None of the above

B.

The smaller the DNA fragment, the A. Harder it is to move down the gel B. Further it moves down the gel C. The more bands you see on the stained gel D. The fewer bands you see on the stained gel

B.

This type of blood has no antigens on its RBC: A. A B. O C. AB D. B

B.

What career involves the study of the symptoms, mechanisms, treatments and detection of poisonings, drugs, and other toxins, especially in people? A. Coroner B. Medical examiner C. EMT D. Toxicologist

B.

What system's main role is waste removal? A. Digestive B. Urinary C. Respiratory D. Endocrine

B.

When running a gel electrophoresis, DNA will migrate towards the ____________ end because DNA has a __________ charge. A. Positive, positive B. Positive, negative C. Negative, negative D. Negative, positive

B.

List three sources of DNA evidence that may be found at a crime scene.

Blood, Hail Follicle, Vomit

Gel electrophoresis is used to separate the fragments of DNA. The larger pieces of DNA will travel _________ through the gel. A. Faster than the smaller fragments B. The same speed as the smaller fragments C. Slower than the smaller fragments D. In the opposite direction of the smaller fragments

C.

How many minutiae do investigators need to find that match between 2 prints to conclude that they came from the same person? A. 4 B. 20 C. 12 D. 0

C.

The dark middle part of hair is known as ___________ and is only present in some hair types. A. Keratin B. Cuticle C. Medulla D. Cortex

C.

Using the Glaister Equation, if a victim's body temperature was measured at 89.4F at 2:00am their time of death was approximately: A. 6:00pm B. 12:00am C. 8:00pm D. 10:00pm

C.

What has a single ring structure? A. Purine B. Nucleotide C. Pyrimidine D. Phosphate group

C.

What property of DNA causes it to migrate to the positive pole of the electrophoresis apparatus? A. The positive charge of the phosphate groups B. The positive charge of the sugars C. The negative charge of the phosphate groups D. The negative charge of the sugars

C.

Which one of the following situations would not be allowed according to the HIPAA Privacy Rules? A. A patient arrives in the Emergency Room and is suspected of having tuberculosis, the physician contacts the health department with the information. B. A nurse suspects that her neighbor is leaving her 7 year old daughter alone in the evening while working and contacts Child Protective Services. C. After a tornado disaster, the medical facility notifies the media on the condition of the mayor's daughter, who was a victim. D. The hospital contacts the police department about a patient with a gunshot wound.

C.

An examination of the body after death that includes a dissection of vital organs to determine that cause of death is a (an) A. Toxicology report B. Death report C. Death certificate D. Autopsy

D.

DNA is cut at specific nucleotide sequences by A. RFLPs B. DNA Proteins C. Gel Electrophoresis D. Restriction Enzymes

D.

Gel electrophoresis separates a small segments of DNA based upon A. Charge of differential of DNA segments B. Relative ratio of purines to pyrimidines in the DNA segments C. Quantity of DNA D. Size of DNA fragments

D.

Scientists are able to produce millions of copies of a specific DNA sequence from small amount of DNA though A. DNA Profiling B. Gel Electrophoresis C. RFLPs D. Polymerase Chain Reaction

D.

This type of blood clumps when mixed with anti-B serum, but not with anti-A: A. A B. O C. AB D. B

D.

Which body system is most closely associated with the support and protection of the body organs? A. Lymphatic B. Musculatory C. Endocrine D. Skeletal

D.

Which body system is most closely associated with transport delivery? A. Nervous B. Integumentary C. Respiratory D. Cardiovascular

D.

How do the clues found at a scene of a mysterious death help investigators determine what might have occurred and help identify or exonerate potential suspects?

DNA, Fingerprints, Hair, Shoe prints

What are the five steps needed to properly process a crime scene? Please list them in order and give a brief description of what that step entails.

Examine crime scene - search systematically, label evidence. Photograph - use a ruler. Sketch - draw a diagram. Interviews - witnesses. Collect evidence - make sure to not contaminate.

What tool is used to load DNA samples in a gel for electrophoresis?

Micropippetor

How do scientists isolate DNA in order to study it?

They first have to break through the cell wall if it is a plant cell, then though the cell and nuclear membranes, then filter and pour in ice cold ethanol.

Name three things that can be determined by examining a shoe print at a crime scene.

Tread, Size, Direction