WFU - Working with the IACUC (Wake Forest University)

Which task is NOT a function of the IACUC?

Assist with writing the basic science portions of a grant application.

Which statement applies to experimental surgery on animals?

General or regional anesthesia is required to prevent pain during surgery.

Which of the following is best used as a source of guidelines for animals housed in farm settings?

Guide for the Care and Use of Agricultural Animals in Research and Teaching

Recombinant DNA inserted into the genome of transgenic mice may NOT be covered by the NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules

True

Protection for those who report misuse of animals or noncompliance is mandated by the Animal Welfare Regulations

true

Some types of changes to an animal protocol can be approved administratively by veterinary verification and consultation.

true

When euthanizing adult mice with carbon dioxide, how long should the animals remain in the container with the gas flowing after they stop breathing?

1 minute

Prior to IACUC review of the animal use protocol application that describes procedures that are expected to cause more than momentary pain or distress, which of the following is mandated by the Animal Welfare Regulations?

A laboratory animal veterinarian must be consulted.

Which of the following statements about using a statistical analysis to justify animal numbers is TRUE?

A statistical analysis should be used whenever possible.

Which of the following institutions must follow PHS guidelines for animal research?

All institutions that accept PHS funding

The PHS Policy applies to which animal species?

All vertebrate species use in research

The PHS Policy differs from the Animal Welfare Act because it applies to which animals?

All vertebrate species used in research

What document provides the guidelines for working with infectious diseases in animal research?

Biosafety in Microbiological and Biomedical Laboratories

Which biosafety level is required for work with dangerous and exotic agents that pose a high individual risk of aerosol-transmitted laboratory infections and life-threatening diseases that are frequently fatal, agents for which there are no vaccines or treatments, or work with a related agent with unknown risk of transmissiom?

BSL-4

When must personnel receive training to perform animal procedures?

Before any procedures are performed

Using an animal for which of the following would prevent it from being placed in USDA category B?

Blood collection

In which USDA pain/distress category would a study be placed if it receives increased IACUC scrutiny and must be reported annually to the USDA?

Category E

Prior to beginning any work involving research animals, an investigator must do which of the following?

Complete an animal use protocol application describing all proposed procedures and obtain IACUC approval.

Which of the following statements concerning the use of carbon dioxide to euthanize rodents is TRUE?

Compared to adults, neonates are resistant to low oxygen levels, and less susceptible to death by carbon dioxide inhalation.

Which of the following is a function of the IACUC?

Conduct reviews of the animal care and use program on a semi-annual basis.

The Animal Welfare Regulations require the IACUC to do what two things regarding alternatives?

Ensure that the principal investigator has considered alternatives to painful or distressing procedures and review a written assurance that alternatives are not available.

What should you do if you see someone treating a laboratory animal in a way that you do not think is ethical?

Find a veterinarian, IACUC staff member, or IACUC chair and tell them what you saw.

The Animal Welfare Act and Regulations currently apply to which of the following animals?

Hamsters

Who is given institutional responsibility for deciding if an individual researcher is properly trained to perform animal procedures, as required by law?

IACUC

Which statement about database searches for animal alternatives is FALSE?

If an alternative is available, it may be disregarded if the lab has more experience with the current technique.

Which statement concerning non-survival surgery is TRUE?

If an animal undergoes non-survival surgery, the surgical site should be clipped and clean instruments used.

All of the following statements concerning survival surgery are true EXCEPT:

If the incision site on the skin is disinfected with iodine or chlorhexidine compounds, hair clipping is not necessary.

Using an animal for which of the following would require it to be placed in USDA category E?

Lethal dose experiments in which animals are allowed to die without intervention

What does the acronym IACUC stand for?

Institutional Animal Care and Use Committee

Endpoint criteria describe when it is time to do which step?

Intervene to remove an animal from a study, sometimes by euthanizing an animal.

Which of the following statements about the use of carbon dioxide for euthanasia is TRUE?

It is not a preferred method of euthanizing rabbits, dogs, cats, and primates.

USDA requires institutions to provide training in all of the following areas EXCEPT which one?

Laboratory animal facility design

Which organization is responsible for monitoring institutional compliance with PHS Policy?

Office of Laboratory Animal Welfare

All of the following statements about the postoperative period are true EXCEPT:

Postoperative analgesia is not generally needed in rodents.

The three Rs of Russell and Burch stand for what?

Replacement, reduction, and refinement

An agent that has high individual risk but low community risk would be in which Risk Group per the NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules?

Risk Group 3

Using an animal for which of the following would prevent it from being placed in USDA category D?

Studies in which animals are allowed to die without pain relief

Which reference document contains detailed guidelines for performing euthanasia on a variety of animals?

The AVMA Guidelines for the Euthanasia of Animals

What must occur if a serious noncompliance occurs?

The institution must report the noncompliance to the Office of Laboratory Animal Welfare at NIH.

Which of the following is an example of reduction in the number of animals used per the 3Rs?

The use of common control groups during simultaneous experiments to limit the number of control animals needed

Which of the following is helpful to the IACUC when reviewing an animal protocol?

The use of a flowchart to illustrate complex experimental designs

Which of the following is an example of replacement?

The use of a kit to assay hormone levels instead of a bioassay involving animals

Which of the following is NOT an example of replacement per the three Rs of Russell and Burch?

The use of a new analgesic that provides better postoperative pain relief than the existing treatment on the protocol

Which procedure is an example of refinement?

The use of a new bleeding technique that causes less pain and distress

Which of the following is an example of refinement per the three Rs of Russell and Burch?

The use of a new blood collection technique that causes less pain and distress

What is the primary goal of the three Rs concept of Russell and Burch?

Urge scientists to minimize use of animals and to minimize pain and distress caused by animal experiments.

All of the following statements about postoperative care records are true EXCEPT:

Written postoperative care records are not necessary as long as proper care is provided and treatments are administered.

Which statement identifies an appropriate approach for maintaining compliance?

You do not need to be an expert in all the rules and regulations, just make sure you read, understand, and follow your approved IACUC protocol

Which statement about the Occupational Health and Safety Program is FALSE?

You may not decline medical treatments provided as part of the Occupational Health and Safety Program.

The principal investigator is responsible for ensuring all staff are trained to:

all of the Above

Which of the following protocol changes require IACUC approval?

all of the above

Explaining the potential value of proposed animal research to human and animal health is:

important to convey using lay language so that the community member of the IACUC can understand it.