Working with IACUC

Non-USDA regulated species are never required to be placed in a pain/distress category.

False

Surgery that penetrates and exposes a body cavity or produces substantial physical impairment is described as what kind of surgery?

Major

Protection for those who report misuse of animals or noncompliance is mandated by the Animal Welfare Regulations

True

Some types of changes to an animal protocol can be approved administratively by veterinary verification and consultation.

True

The USDA requires documentation of all postoperative care and retention of records for one year after the death of the animal.

True

Thermal support is an important aspect of postoperative support

True

Which of the following is a role of AAALAC International?

To conduct voluntary evaluations of institutional animal care and use programs.

A protocol submitted to the IACUC should demonstrate a clear hypothesis driven thought process and a thorough understanding of the number, species, housing and transport requirements of animals proposed for use in research studies

True

All significant changes to an animal use protocol need to be approved by the IACUC

True

Environmental enrichment should be applied consistently to all animals that are part of a study

True

Which types of animals must have access to environmental enrichment, as mandated by the Animal Welfare Regulations?

dogs and nonhuman primates

Enrollment in the institutional Occupational Health and Safety program is mandatory before working with animals

false

Restraint for brief periods of time during experimental procedures do not need to be mentioned in the animal use protocol

false

Adjunctive methods of euthanasia, such as pithing frogs, should only be performed on animals that are already unconscious.

true

Methods of euthanasia deemed "acceptable with conditions" may require scientific justification before IACUC approval.

true

Recombinant DNA inserted into the genome of transgenic mice may NOT be covered by the NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules

true

Which of the following protocol changes require IACUC approval?

-Changing the procedure in a manner that would increase the pain category -Adding additional time points to an experiment -Changing drug dosage

Functions of IACUC

-Conduct reviews of the animal care and use program on a semi-annual basis. -Review and approve animal use protocols submitted by investigators. -Inspect the animal facilities on a semi-annual basis.

Reasons to provide environmental enrichment?

-To increase the ability of animals to cope with the stress of experimental manipulation -To encourage positive behaviors -Health of the animals

The principal investigator is responsible for ensuring all staff are trained to:

-recognize the hazards involved in working with animals such as zoonoses and allergy. -correctly wear personal protective equipment. -understand the health care services offered by the Occupational Health and Safety program.

What are some reasons for single housing social animals?

-scientific reasons -health problems -fighting or aggressive behaviors

A simple rule to prevent excess blood collection in an animal is to collect no more than what percentage of body weight as blood at one time?

1%

When does the postoperative period end?

10-14 days

Which of the following statements about using a statistical analysis to justify animal numbers is TRUE?

A statistical analysis should be used whenever possible.

Which of the following institutions must follow PHS guidelines for animal research?

All institutions that accept PHS funding Return to Lesson

Which of statement is true about the euthanasia of neonatal mice?

An anesthetic overdose is an acceptable method.

What document provides the guidelines for working with infectious diseases in animal research?

Biosafety in Microbiological and Biomedical Laboratories

Which of the following activities would EXCLUDE an animal from USDA category B?

Blood collection

Which of the following are NOT allowed by USDA?

Changes in animal ownership and movement of animals between facilities during the postoperative period

The Animal Welfare Regulations require the IACUC to do what two things regarding alternatives?

Ensure that the principal investigator has considered alternatives to painful or distressing procedures, and review a written assurance that alternatives are not available.

Which statement concerning survival experimental surgery is FALSE?

If the incision site on the skin is disinfected with iodine or chlorhexidine compounds, hair clipping is not necessary.

What does IACUC stand for?

Institutional Animal Care and Use Committee

Endpoint criteria describe when it is time to do what?

Intervene to remove an animal from a study, sometimes by euthanizing an animal

Which of the following activities WOULD place an animal in category E?

Lethal dose experiments in which animals are allowed to die without intervention

Which of the statements concerning blood collection in mice and rats is TRUE?

Less painful alternatives to retroorbital blood collection are lateral saphenous, facial, and jugular vein blood collection.

Which parameter would NOT likely be part of an endpoint policy?

Number of animals approved by the IACUC for a particular protocol

Which organization is responsible for monitoring institutional compliance with PHS Policy?

Office of Laboratory Animal Welfare

The Johns Hopkins Center for Alternatives to Animal Testing (CAAT), the Institute for In Vitro Sciences, and the European Center for Validation of Alternative Methods (ECVAM) are primarily interested in what area?

Performing or sponsoring research into alternatives to animal use for research and testing.

Which of the following statements about the postoperative period is FALSE?

Postoperative analgesia is generally unnecessary in rodents.

The three Rs of Russell and Burch stand for what?

Replacement, reduction, and refinement

Which of the following statements regarding the use of restraint on animals in a research study is FALSE?

Restraint can be used as a normal component of animal housing

Which of the following activities would EXCLUDE an animal from USDA category D?

Studies in which animals are allowed to die without pain relief

Methods for substance administration in rats and mice are outlined in which document?

The Guide

Who is generally given institutional responsibility for deciding if an individual researcher is properly trained to perform animal procedures, as required by law?

The IACUC

Which of the following is TRUE regarding the Animal Welfare Regulations and duplication of experiments?

Unnecessary duplication of animal experiments is not allowed.

Which of the following is an example of refinement per the three Rs of Russell and Burch?

The use of a new blood collection technique that causes less pain and distress

Which of the following is helpful to the IACUC when reviewing an animal protocol?

The use of a flowchart to illustrate complex experimental designs

Which of the following is NOT an example of reduction per the three Rs of Russell and Burch?

The use of a new anesthetic that provides better induction and a shorter recovery period

What is a primary mission of the Animal Welfare Information Center (AWIC), a part of USDA?

To provide assistance with alternatives and database searches.

What is the basis for the pain/distress classification system used by most institutions?

USDA pain/distress categories

Which observation might indicate pain and thus the need for postoperative analgesia?

Unusual Posture

Which activity would place a study in pain category B?

Use as a breeder

Which of the following statements concerning selection of species for research is FALSE?

Vertebrate species should be used instead of invertebrates whenever possible.

Which of the following would NOT be accepted by an IACUC as the sole justification for performing multiple major survival surgeries?

cost savings

An agent that has high individual risk but low community risk would be in which Risk Group per the NIH Guidelines for Research Involving Recombinant or Synthetic Nucleic Acid Molecules?

risk group 3

Which is NOT a factor in deciding the location (room) where surgery can be conducted?

the experience of the surgeon