ANOVA simplified

t test

"ANOVA's younger sibling."

What are the # of levels? Ex: You want to know how gait speed varies based on age and gender. Define IV, DV, and levels.

# of groups within each independent variable DV: Gait speed IV: Age and Gender 3 levels (groups) for age 2 levels (groups) for gender

What are the three assumptions of ANOVA?

1. independence of observations 2. normality 3. homogeneity of variance

What is a 2 way ANOVA?

2 independent variables

Sum of Squares Error (SSE)

A value that measures the variation in a dependent variable that is explained by variables other than an independent variable.

T-test or ANOVA A group of psychiatric patients are trying three different therapies: counseling, medication and biofeedback. You want to see if one therapy is better than the others.

ANOVA

What is an ANOVA?

ANOVA is an analysis of variance: * between groups (or levels of a factor) or * within groups (or error) you're testing groups to see if there's a difference between them

What is the F statistic or F ratio?

ANOVA value - identifies if the means between two populations are significantly different.

what type of ANOVA test? you have one group taking two tests

ANOVA without replication

What is the null hypothesis for ANOVA?

All means are equal μ1 = μ2 = μ3 = μ4

What do main effect and interaction effect have in common?

Assessing effect of the IV

You want to test for sex differences in gait velocity among 3 age groups used earlier. What is the "main effect"?

Average effect for each independent variable. Main effect for sex (combing all ages) "What is the effect of sex on gait speed? Is there a difference in gait speed if you're male versus female? Do men and women have different gaits speeds?" Main effect for age (combining both genders)? "Does gait speed depend on age?"

degrees of freedom

Degrees of freedom of an estimate is the number of independent pieces of information that went into calculating the estimate. It's not quite the same as the number of items in the sample. In order to get the df for the estimate, you have to subtract 1 from the number of items. Another way to look at degrees of freedom is that they are the number of values that are free to vary in a data set. Degrees of freedom becomes a little more complicated in ANOVA tests. Instead of a simple parameter (like finding a mean), ANOVA tests involve comparing known means in sets of data. For example, in a one-way ANOVA you are comparing two means in two cells. The grand mean (the average of the averages) would be: Mean 1 + mean 2 = grand mean. What if you chose mean 1 and you knew the grand mean? You wouldn't have a choice about Mean2, so your degrees of freedom for a two-group ANOVA is 1.

Mean Square Among Groups (MSA) formula

Divide the SSA by the number of groups minus 1.

MSW formula

Divide the SSW by the number of cases in the total sample minus the number of groups.

You want to test for sex differences in gait velocity among 3 age groups used earlier. What is the interaction asking?

Does the effect of gender (on gait speed) DEPEND on how old you are? Does the effect of age (on gait speed) DEPEND on sex? or: For gait speed, is there a different trend of increasing age for women versus men?

partial eta squared

Eta squared is the proportion of variance associated with one or more main effects, errors or interactions in ANOVA. In other words, we know there is a difference but how big a difference?

A one way ANOVA is used to compare two means from two independent (unrelated) groups using the ____.

F-distribution

A(n) ____ is computed for each hypothesis you are testing.

F-statistic

p value

F-statistic must be used in combination with the p-value when you are deciding if your overall results are significant. Why? If you have a significant result, it doesn't mean that all your variables are significant. The statistic is just comparing the joint effect of all the variables together. If the p value is less than the alpha level, go to Step 2 (otherwise your results are not significant and you cannot reject the null hypothesis). A common alpha level for tests is 0.05. Study the individual p values to find out which of the individual variables are statistically significant.

when you are investigating if there is an interaction between income and gender for anxiety level at job interviews categorical variable = ____

Gender and Income

Assumptions for Two Way ANOVA (4) · The population must be close to a normal distribution. · Samples must be independent. · Population variances must be equal. · ____

Groups must have equal sample sizes.

variability

How do things differ from the average? · variance · standard deviation · range · interquartile range

t test versus f test

Hypothesis testing starts with setting up the premises, which is followed by selecting a significance level. Next, we have to choose the test statistic, i.e. t-test or f-test. While t-test is used to compare two related samples, f-test is used to test the equality of two populations. T-test is a univariate hypothesis test, that is applied when standard deviation is not known and the sample size is small. F-test is statistical test, that determines the equality of the variances of the two normal populations.

Covariate

In general terms, covariates are characteristics (excluding the actual treatment) of the participants in an experiment. If you collect data on characteristics before you run an experiment, you could use that data to see how your treatment affects different groups or populations. Or, you could use that data to control for the influence of any covariate. Covariates may affect the outcome in a study. For example, you are running an experiment to see how corn plants tolerate drought. Level of drought is the actual "treatment", but it isn't the only factor that affects how plants perform: size is a known factor that affects tolerance, so you would run plant size as a covariate. A covariate can be an independent variable (i.e. of direct interest) or it can be an unwanted, confounding variable. Adding a covariate to a model can increase the accuracy of your results.

What is the interaction ?

Is there an interaction BETWEEN the independent variables? Analyze all subgroups for significant differences

ways to correct for family wise type I error

LSD (least significant difference), Bonferroni, Sidak

If your one-way ANOVA returns a significant f-statistic, you may run the ____ test to tell you exactly which groups had a difference in means.

Least Significant Difference

The F-test is just the ratio of the ____ and ____

MSA MSW

symbols

N = total sample size; n = subsample size; SS = sum of squares; MS = mean square; df = degree of freedom; a = number of groups (or levels for a categorical variable (or factor); used in calculation of some df; Y = the dependent variable; u (mu) = mean; i = identifying number of an individual within a group (or level); j = identifying number of a group or level

When do you look at post-hoc tests?

ONLY IF overall F value is significant

Observed Power

Observed power (or post-hoc power) is the statistical power of the test you have performed, based on the effect size estimate from your data. Statistical power is the probability of finding a statistical difference from 0 in your test (aka a 'significant effect'), if there is a true difference to be found.

You want to test for sex differences in gait velocity among 3 age groups used earlier. What is the null hypothesis for each main effect? What is the null for the interaction?

One null for each main effect: Age: μ1 = μ2 = μ3 Sex: μ males = μ females One null for the interaction: μ1 males = μ1 females = μ2 males = μ2 females = μ3 males = μ3 females

what type of ANOVA test? you want to test two groups to see if there's a difference between them.

One-way ANOVA between groups

One-way test has how many independent variables and levels?

One-way has one independent variable (with 2 levels)

Assumptions for Two Way ANOVA (4) · The population must be close to a normal distribution. · Samples must be independent. · ____ · Groups must have equal sample sizes.

Population variances must be equal.

post hoc test

Post-hoc (Latin, meaning "after this") means to analyze the results of your experimental data. They are often based on a familywise error rate; the probability of at least one Type I error in a set (family) of comparisons. The most common post-hoc tests are: Bonferroni Procedure Duncan's new multiple range test (MRT) Dunn's Multiple Comparison Test Fisher's Least Significant Difference (LSD) Holm-Bonferroni Procedure Newman-Keuls Rodger's Method Scheffé's Method Tukey's Test (see also: Studentized Range Distribution) Dunnett's correction Benjamin-Hochberg (BH) procedure

What is the purpose of a post-hoc test?

Purpose: Discover which pairs of scores are significantly different -Preserves FAMILY-WISE PROTECTION against Type I error

f value in ANOVA

SPSS calculates the F value. The F value in one way ANOVA is a tool to help you answer the question "Is the variance between the means of two populations significantly different?" The F value in the ANOVA test also determines the P value; The P value is the probability of getting a result at least as extreme as the one that was actually observed, given that the null hypothesis is true. The p value is a probability, while the f ratio is a test statistic, calculated as: F value = variance of the group means (Mean Square Between) / mean of the within group variances (Mean Squared Error)

SSW formula

SST-SSA

Assumptions for Two Way ANOVA (4) · The population must be close to a normal distribution. · ____ · Population variances must be equal. · Groups must have equal sample sizes.

Samples must be independent.

Effect Size

Statistical testing is not enough. With large samples (lots of power) very small effects can be significant...but are they important? Effect sizes help us to decide. The terms "Measure of Association" and "Effect Size" both mean the same thing: quantifying the relationship between two groups. It's more common to talk about Effect Size in the medical field, when you want to know how exposure is related to disease (i.e. What effect does exposure have on disease outcome?). On the other hand, Measure of Association is used in an informal way to mean the same thing (quantifying relationships between groups) in most other fields. The effect size is how large an effect of something is. For example, medication A is better than medication B at treating depression. But how much better is it? A traditional hypothesis test will not give you that answer. Medication B could be ten times better, or it could be slightly better. This variability (twice as much? ten times as much?) is what is called an effect size. Effect Size (Measures of Association) Definition and Use in Research Statistics Definitions > Effect Size / Measurement of Association Before reading this article, you may want to review: What is a p value?. The terms "Measure of Association" and "Effect Size" both mean the same thing: quantifying the relationship between two groups. It's more common to talk about Effect Size in the medical field, when you want to know how exposure is related to disease (i.e. What effect does exposure have on disease outcome?). On the other hand, Measure of Association is used in an informal way to mean the same thing (quantifying relationships between groups) in most other fields. Measure of association could also refer to specific tests for relationships, like: Chi square test of independence, Odds ratio, Proportionate mortality ratio Rate ratio, Risk Ratio (relative risk). Effect Size: Overview The effect size is how large an effect of something is. For example, medication A is better than medication B at treating depression. But how much better is it? A traditional hypothesis test will not give you that answer. Medication B could be ten times better, or it could be slightly better. This variability (twice as much? ten times as much?) is what is called an effect size. Most statistical research includes a p value; it can tell you which treatment, process or other investigation is statistically more sound than the alternative. But while a p value can be a strong indicator of which choice is more effective, it tells you practically nothing else. Effect size can tell you: How large the difference is between groups. The absolute effect (the difference between the average outcomes of two groups). What the standardized effect size is for an outcome. Three common measures in ANOVA are: Omega squared, Epsilon squared, Eta squared.

SSA stands for

Sum of Squares among Groups

For ANOVA three types of sums of squares are calculated ____, ____ and ____

TOTAL WITHIN BETWEEN

Estimated Marginal Means

The Estimated Marginal Means in SPSS GLM tell you the mean response for each factor, adjusted for any other variables in the model. If all factors (aka categorical predictors) were manipulated, these factors should be independent. Or at least they will be if you randomly assigned subjects to conditions well.

general linear model

The General Linear Model (GLM) comparing how several variables affect different continuous variables. Data = Model + Error test statistic (degree to which data depart from the expected, null hypothesis; based on the sums of squares Is the variability between groups greater than that expected on the basis of the within-group variability?

Assumptions for Two Way ANOVA (4) · ____ · Samples must be independent. · Population variances must be equal. · Groups must have equal sample sizes.

The population must be close to a normal distribution.

sum of squares

The sum of the squared deviations, (X-Xbar)², is also called the sum of squares or more simply SS. SS represents the sum of squared differences from the mean and is an extremely important term in statistics. In a regression analysis, the goal is to determine how well a data series can be fitted to a function which might help to explain how the data series was generated. In the context of ANOVA, this quantity is called the total sum of squares (abbreviated SST) because it relates to the total variance of the observations.

What does a SIGNIFICANT INTERACTION tell us?

This tells us that any main effects may be MISLEADING or MEANINGLESS

Two-way ANOVA when placing one observation in each cell, how many null hypotheses are tested?

Two null hypotheses

What test? when you are investigating if there is an interaction between income and gender for anxiety level at job interviews

Two-way ANOVA

What test? when you have a quantitative outcome and you have two categorical explanatory variables,

Two-way ANOVA

What test? when you have one measurement variable (i.e. a quantitative variable) and two nominal variables.

Two-way ANOVA

what type of ANOVA test? you're testing two groups of patients from different hospitals trying two different therapies.

Two-way ANOVA with replication Two groups, and the members of those groups are doing more than one thing.

what type of ANOVA test? you're testing one set of individuals before and after they take a medication to see if it works or not.

Two-way ANOVA without replication you have one group and you're double-testing that same group.

Univariate

Univariate analysis is the simplest form of analyzing data. "Uni" means "one", so in other words your data has only one variable. It doesn't deal with causes or relationships (unlike regression) and it's major purpose is to describe; it takes data, summarizes that data and finds patterns in the data. Some ways you can describe patterns found in univariate data include central tendency (mean, mode and median) and dispersion: range, variance, maximum, minimum, quartiles (including the interquartile range), and standard deviation. You have several options for describing data with univariate data. Click on the link to find out more about each type of graph or chart: Frequency Distribution Tables. Bar Charts. Histograms.

If your groups or levels have a hierarchical structure (each level has unique subgroups), then use ____ for the analysis.

a nested ANOVA

ordinal variable

a qualitative variable that incorporates an order position, or ranking; ordinal scale examples: class rankings, SES, Likert scale

skewness

a statistical measure indicating the symmetry of the distribution around the mean; within +- 2: normal

categorical variable

a variable that names categories examples: hair color, gender

Two-way ANOVA the interaction effect: ____.

all factors are considered at the same time

If you have a significant result, it doesn't mean that ____ are significant

all your variables

Sum of Squares ____ measures how the group means vary from the overall means

among Groups

MSW represents the ____ within the groups.

amount of variation of the scores

when you are investigating if there is an interaction between income and gender for anxiety level at job interviews outcome = ____.

anxiety level the variable that can be measured

standard deviation

average deviation from the mean

MSA represents the amount of difference ____ the groups.

between

post hoc comparisons

comparisons explored afterwards

pairwise comparisons

comparisons of each possible pair of means; each comparison has its own new null hypothesis

independent sample t test

comparisons of the means of two independent variables with two levels, Example: Two independent samples of high school seniors (60 boys; 60 girls) to see if there are gender differences on vocab test.

a priori comparisons

comparisons planned beforehand; if hypotheses is truly a priori we do not need to correct for family wise Type I error

psi equation

contrast expressed as an equation; contrast the weight and mean of a level of the IV

For ANOVA we partition the total variance into how group means ____ and how individual observations within groups differ from ____.

differ from the grand mean their group's mean

What are "Groups" or "Levels"?

different groups in the same independent variable

Two-way ANOVA the main effect: ____.

each factor's effect is considered separately

Measure how observations vary within their group: Sum of Squares ____.

error

mean square

estimates of variance across groups. sum of squares divided by its degrees of freedom.

Three basic types of quantitative research designs

experimental (random, equal groups); quasi-experimental (may use random; may have self selection); observational/phenomenological/descriptive

type II error

false negative (e.g. very pregnant woman told not pregnant)

type I error

false positive (e.g. male pregnant)

SSA measures how the ____ means vary from the ____ means

group overall

orthogonality

helps keep us honest and sane; ensures we do not violate the spirit of a priori contrasts, not redundant

kurtosis

how flat or peaked a normal distribution is; within +- 2: normal

If the group means are dispersed the SSA ____.

increases

In a two-way ANOVA categorical variables are also the ____, which are called ____

independent variables factors

Study the individual p-values to find out which of the ____ are statistically significant.

individual variables

Two-way ANOVA the ____ effect: all factors are considered at the same time.

interaction

family wise error

is the probability of making one or more false discoveries, or type I errors when performing multiple hypotheses tests.

you can compare the f-value with an f critical value in the table. If your observed value of F is ____ than the value in the F table you can reject the null hypothesis with 95 percent confidence that the variance between your two populations ____ due to random chance.

larger isn't

If the p-value is ____ the alpha level reject the null hypothesis and go to Step 2 .

less than

The factors can be split into ____. Income could be split into: low, middle and high income.

levels

The ____ effect: each factor's effect is considered separately.

main

The results from a Two Way ANOVA will calculate a(n) ____ effect and a(n) ____effect.

main interaction

If the p-value is ____ the alpha level, your results are not significant and you cannot reject the null hypothesis.

more than

The Sum of Squares Total represents the overall variability in our ____.

outcome variable

total sum of squares (SST) is the sum, ____, of the squared differences of each observation from the overall mean.

over all observations

The Sum of Squares Total represents the ____ in our outcome variable

overall variability

F-statistic must be used in combination with the ____ when you are deciding if your overall results are significant.

p-value

omnibus test

permits analysis of several variables or variable levels at the same time; in ANOVA, can use F test for differences between groups

Two-way ANOVA Two null hypotheses are tested if you are ____. H01: All the income groups have equal mean stress. H02: All the gender groups have equal mean stress.

placing one observation in each cell

alpha level

probability required for significance; aka rejection rule; usually .05

What Does "One-Way" or "Two-Way Mean?

refers to the number of independent variables (IVs) in your Analysis of Variance test.

SS represents the sum of ____.

squared differences from the mean

variance

sum of squared deviations

The sum of the squared deviations, (X-Xbar)², is also called ____.

sum of squares (SS).

Difference between t-tests and ANOVA

t-tests compare only two sample distributions, ANOVA is capable of comparing many.

The F-test is a ratio of the variance between the group means relative to ____.

the amount of variation in the sample

a "residual" is a measure of ____.

the distance from a data point to a regression line

independent variable (IV)

the factor being manipulated by the experimenter; the thing we think affects other things; can be continuous, ordinal, or categorical also called factors or effects; each IV - greater than or equal to 2 levels

Two-way ANOVA For multiple observations in cells, how many null hypotheses are being tested?

three H03: The factors are independent or the interaction effect does not exist.

what type of ANOVA test? two groups of students from two colleges taking two tests.

two-way ANOVA with replication you have two groups and individuals within that group are doing more than one thing

Two-way test has how many independent variables and levels?

two-way has two independent variables (can have multiple levels).

paired sample t test

usually based on groups of individuals who experience both conditions of the variable of interest. For instance, one study might examine the effects of Drug A versus Drug B on a single sample of 100 diabetics. Subjects in this sample would receive Drug A one week, and Drug B the next; participants receive both drug/stimulus conditions

continuous variable

variable that takes on an infinite number of different values examples: time, weight, income, age.

What Does "Replication" Mean?

whether you are replicating your test(s) with multiple groups.

MSW represents the amount of variation of the scores ____ the groups.

within

SSE measures ____ group variation.

within

If all the group means are equal SSA is ____.

zero

family-wise error rate

· FWE or FWER · the probability of a coming to at least one false conclusion in a series of hypothesis tests. · it's the probability of making at least one Type I Error. · also called alpha inflation or cumulative Type I error.

total sum of squares

· Indicates you how much variation there is in the dependent variable. · the sum, over all observations, of the squared differences of each observation from the overall mean.

What is the mean square formula?

· Mean Square= SS divided by its DF · In ANOVA, mean squares are used to determine whether factors (treatments) are significant. · Represents the variation between the sample means.

SST formula

· Subtract each of the scores from the mean of the entire sample. · Square each of those deviations. · Add those up for each group, · Add the groups together.

Assumptions for Two Way ANOVA (4)

· The population must be close to a normal distribution. · Samples must be independent. · Population variances must be equal. · Groups must have equal sample sizes.

You need to use the p-value in conjunction with the f-statistic because f-statistic is just comparing the____ and doesn't indicate that ____.

· joint effect of all the variables together. · all the variables are significant

statistically significant

· significant result - your results likely did not happen by chance. · no statistically significant results - you can't reject the null hypothesis - throw test data out. · if your calculated F value in a test is larger than your F statistic, you can reject the null hypothesis. · should also consider the p-value. · The p-value (determined by the F-statistic) is the probability your results could have happened by chance.

How are t-tests and f-tests different?

· t-test - whether a single variable is statistically significant · f-test whether a group of variables are jointly significant.

dependent variable (DV; Y)

· the factor being measured (i.e., the result of · interest); · the thing we think is affected by other things; · always measured; · can be continuous, ordinal, or categorical

Sum of squares measures ____ between ____ and ____.

· the overall difference · your data · the values predicted by your estimation model

What is the Alternative (one way) hypothesis?

μ1≠ μ2≠ μ3≠ μ4 (There is at least ONE difference between groups)