AP Bio - Chapter 7 Mastering

reactants of glycolysis

Glucose and 2 ATP

products of glycolysis

2 pyruvate, 2 ATP, 2 NADH

The electron acceptor

oxidizing agent

Suppose that a cell's demand for ATP suddenly exceeds its supply of ATP from cellular respiration. Which statement correctly describes how this increased demand would lead to an increased rate of ATP production?

ATP levels would fall at first, decreasing the inhibition of PFK and increasing the rate of ATP production. An increased demand for ATP by a cell will cause an initial decrease in the level of cellular ATP. Lower ATP decreases the inhibition of the PFK enzyme, thus increasing the rate of glycolysis, cellular respiration, and ATP production. It is the initial decrease in ATP levels that leads to an increase in ATP production.

Pyruvate is oxidized

After glycolysis but before the citric acid cycle in the matrix

In the sequential reactions of acetyl CoA formation and the citric acid cycle, pyruvate (the output from glycolysis) is completely oxidized, and the electrons produced from this oxidation are passed on to two types of electron acceptors. Drag the labels on the left to show the net redox reaction in acetyl CoA formation and the citric acid cycle. Note that two types of electron carriers are involved.

As in glycolysis, the electrons removed from carbon-containing intermediates during acetyl CoA formation and the citric acid cycle are passed to the electron carrier NAD+, reducing it to NADH. The citric acid cycle also uses a second electron carrier, FAD (flavin adenine dinucleotide), the oxidized form, and FADH2, the reduced form.

How would anaerobic conditions (when no O2O2 is present) affect the rate of electron transport and ATPATP production during oxidative phosphorylation? (Note that you should not consider the effect on ATPATP synthesis in glycolysis or the citric acid cycle.)

Both electron transport and ATP synthesis would stop. Oxygen plays an essential role in cellular respiration because it is the final electron acceptor for the entire process. Without O2, mitochondria are unable to oxidize the NADH and FADH2 produced in the first three steps of cellular respiration, and thus cannot make any ATP via oxidative phosphorylation. In addition, without O2 the mitochondria cannot oxidize the NADH and FADH2 back to NAD+ and FAD, which are needed as inputs to the first three stages of cellular respiration.

oxidation-reduction reactions, or redox reactions

Chemical reactions that transfer electrons between reactants Some redox reactions do not transfer electrons but change the electron sharing in covalent bonds

The ATP that is generated in glycolysis is produced by substrate-level phosphorylation, a very different mechanism than the one used to produce ATP during oxidative phosphorylation. Phosphorylation reactions involve the addition of a phosphate group to another molecule. Sort the statements into the appropriate bin depending on whether or not they correctly describe some aspect of substrate-level phosphorylation in glycolysis.

Correct: -One of the substrates is a molecule derived from the breakdown of glucose -An enzyme is required in order for the reaction to occur -A bond must be broken between an organic molecule and phosphate before ATP can form.as Incorrect: -The phosphate group added to ADP to make ATP comes from free inorganic phosphate ions. -The enyzmes involved in ATP synthesis must be attached to a membrane to produce ATP. In substrate-level phosphorylation, an enzyme transfers a phosphate group from one molecule (an intermediate in the breakdown of glucose to pyruvate) to ADP to form ATP. This is very different from the mechanism of ATP synthesis that takes place in oxidative phosphorylation.

NADH and FADH2 are both electron carriers that donate their electrons to the electron transport chain. The electrons ultimately reduce O2 to water in the final step of electron transport. However, the amount of ATP made by electrons from an NADH molecule is greater than the amount made by electrons from an FADH2 molecule. Which statement best explains why more ATP is made per molecule of NADH than per molecule of FADH2?

Fewer protons are pumped across the inner mitochondrial membrane when FADH2 is the electron donor than when NADH is the electron donor. Electrons derived from the oxidation of FADH2 enter the electron transport chain at Complex II, farther down the chain than electrons from NADH (which enter at Complex I). This results in fewer H+H+ ions being pumped across the membrane for FADH2 compared to NADH, as this diagram shows. Thus, more ATP can be produced per NADH than FADH2.

Under anaerobic conditions (a lack of oxygen), glycolysis continues in most cells despite the fact that oxidative phosphorylation stops, and its production of NAD+ (which is needed as an input to glycolysis) also stops. The diagram illustrates the process of fermentation, which is used by many cells in the absence of oxygen. In fermentation, the NADH produced by glycolysis is used to reduce the pyruvate produced by glycolysis to either lactate or ethanol. Fermentation results in a net production of 2 ATP per glucose molecule. During strenuous exercise, anaerobic conditions can result if the cardiovascular system cannot supply oxygen fast enough to meet the demands of muscle cells. Assume that a muscle cell's demand for ATP under anaerobic conditions remains the same as it was under aerobic conditions. What would happen to the cell's rate of glucose utilization?

Glucose utilization would increase a lot. ATP made during fermentation comes from glycolysis, which produces a net of only 2 ATP per glucose molecule. In contrast, aerobic cellular respiration produces about 30 ATP per glucose molecule. To meet the same ATP demand under anaerobic conditions as under aerobic conditions, a cell's rate of glycolysis and glucose utilization must increase about 15-fold.

Under anaerobic conditions (a lack of oxygen), the conversion of pyruvate to acetyl CoA stops. Which of these statements is the correct explanation for this observation?

In the absence of oxygen, electron transport stops. NADH is no longer converted to NAD+, which is needed for the first three stages of cellular respiration. NAD+ couples oxidative phosphorylation to acetyl CoA formation. The NAD+ needed to oxidize pyruvate to acetyl CoA is produced during electron transport. Without O2, electron transport stops, and the oxidation of pyruvate to acetyl CoA also stops because of the lack of NAD+.

In glycolysis, as in all the stages of cellular respiration, the transfer of electrons from electron donors to electron acceptors plays a critical role in the overall conversion of the energy in foods to energy in ATP. These reactions involving electron transfers are known as oxidation-reduction, or redox, reactions. Drag the words on the left to the appropriate blanks on the right to complete the sentences.

In the net reaction for glycolysis, glucose (the electron donor) is oxidized to pyruvate. The electrons removed from glucose are transferred to the electron acceptor, NAD+, creating NADH.

In the oxidation of pyruvate to acetyl CoA, one carbon atom is released as CO2. However, the oxidation of the remaining two carbon atoms—in acetate—to CO2 requires a complex, eight-step pathway—the citric acid cycle. Consider four possible explanations for why the last two carbons in acetate are converted to CO2 in a complex cyclic pathway rather than through a simple, linear reaction. Use your knowledge of the first three stages of cellular respiration to determine which explanation is correct.

It is easier to remove electrons and produce CO2 from compounds with three or more carbon atoms than from a two-carbon compound such as acetyl CoA. Although it is possible to oxidize the two-carbon acetyl group of acetyl CoA to two molecules of CO2, it is much more difficult than adding the acetyl group to a four-carbon acid to form a six-carbon acid (citrate). Citrate can then be oxidized sequentially to release two molecules of CO2.

reducing agent

The electron donor

glycolysis -> oxidative phosphorylation -> Acetyl CoA formation

The main coupling among the stages of cellular respiration is accomplished by NAD+ and NADH. In the first three stages, NAD+ accepts electrons from the oxidation of glucose, pyruvate, and acetyl CoA. The NADH produced in these redox reactions then gets oxidized during oxidative phosphorylation, regenerating the NAD+ needed for the earlier stages.

reduction

a substance gains electrons, or is reduced (the amount of positive charge is reduced)

oxidation

a substance loses electrons, or is oxidized

Krebs Cycle (Citric Acid Cycle)

finishes breakdown of pyruvate to CO2

Among the products of glycolysis, which compounds contain energy that can be used by other biological reactions?

pyruvate, ATP, and NADH

When the protein gramicidin is integrated into a membrane, an H+H+ channel forms and the membrane becomes very permeable to protons (H+H+ ions). If gramicidin is added to an actively respiring muscle cell, how would it affect the various processes involved in cellular respiration and oxidative phosphorylation? (Assume that gramicidin does not affect the production of NADHNADH and FADH2FADH2 during the early stages of cellular respiration.) Sort the labels into the correct bin according to the effect that gramicidin would have on each process.

remains the same: rate of oxygen uptake, electron transport rate decreases (or goes to zero): size of the proton gradient, rate of ATP synthesis Gramicidin causes membranes to become very leaky to protons, so that a proton gradient cannot be maintained and ATPATP synthesis stops. However, the leakiness of the membrane has no effect on the ability of electrons to move along the electron transport chain. Thus, the rates of electron transport and oxygen uptake remain unchanged.

In mitochondrial electron transport, what is the direct role of O2?

to function as the final electron acceptor in the electron transport chain. The only place that O2 participates in cellular respiration is at the end of the electron transport chain, as the final electron acceptor. Oxygen's high affinity for electrons ensures its success in this role. Its contributions to driving electron transport, forming a proton gradient, and synthesizing ATPATP are all indirect effects of its role as the terminal electron acceptor.