BIO240 Chapter 23 Genomics I: Analysis of DNA
Arrange the steps involved in fluorescence in situ hybridization (FISH) in the correct order 1)Add fluorescently labeled avidin 2)Hybridize chromosomal DNA to single-stranded DNA probes containing biotin. 3)Treat cells with agents that cause them to swell and fix them onto a slide. 4)View with a fluorescence microscope. 5)Denature chromosomal DNA
3,5,2,1,4
How is genome sequencing likely to improve our understanding of human disease? A) It may lead to the identification of human genes involved in disease. B) It will enable us to prevent new mutations from occurring in the human genome. C) It can provide information about genes found in pathogens that infect humans.
A&C
Understanding of soil microorganisms that facilitate plant growth could benefit
Agriculture
Developing technology for the management of human genome information was one of the goals of the Human Genome Project. To meet this goal, the Human Genome project developed
Analytical tools for interpreting genome information
A cloning vector that can accommodate a large DNA insert and behave like a chromosome when it is inside a living cell is called an ____________ chromosome
Artificial
The distance between two different microsatellites can be determined by calculating the frequency of ______________ between them
Crossing over
The type of mapping in which a gene is localized to a site within a chromosomal banding pattern is ______________ mapping.
Cytogenetic
What type of chromosome mapping relies on microscopy?
Cytogenetic Mapping
In situ hybridization is a technique that is often used for
Cytogenetic mapping
A technique called chromosome painting can be used to
Determine the location of several fluorescently labeled probes simultaneously
T/F: Molecular markers exist in only one form within a given population
False
One of the technological innovations that has made high-throughput sequencing possible is the ability to automate the reading of a DNA sequencing when ______________ labeled nucleotides are used in the DNA sequencing reaction.
Fluorescent
What was the first organism to have it genome completely sequenced?
Haemophilus influenzae (a bacterium)
A cosmid is a hybrid between
Phage lamda DNA and a plasmid
In linkage mapping, the distance between sites on the same chromosome is determined by calculating the frequency of
Recombinant offspring
What is the goal of physical mapping?
To determine the order of overlapping DNA clones from a chromosome
What is the goal of cytogenetic mapping?
To localize a gene to a site within a chromosomal banding pattern
An organism's genome is its
Total genetic composition
The source DNA of YAC is
Yeast DNA
Cloning many segments of human chromosomal DNA into YACs, BACs, and cosmids was an important step in obtaining a _________________ map of the human genome
physical
A molecular marker is a ___________ found at a specific site on a chromosome that has properties that allow it to be _______________
segment of DNA; uniquely identified using molecular tools
What were the goals of the Human Genome Project?
-To obtain the DNA sequence of the entire human genome -To develop technology for the management of human genome information -To analyze the genomes of model organisms
Which of the following causes of genetic disorders can be detected with FISH analysis? 1) Translocations 2) Changes in DNA methylation patterns 3) Duplications 4) Point mutations 5) Deletions
1,3,&5
How many contigs are required for a complete physical map of the human nuclear genome?
24
A prokaryotic genome is about 4 million bp in length. About how many genes would you expect it to contain?
4,000
What is a sequence-tagged site?
A DNA sequence that can be amplified uniquely by PCR
What is an artificial chromosome
A cloning vector that can accept large DNA inserts and be passed on like a chromosome in a living cell.
What is a metagenome
A collection of genes from an environmental sample
What is a molecular marker?
A segment of DNA that can be uniquely identified using molecular tools
What is a contig?
A series of vectors that contain inserts that have overlapping regions of chromosomal DNA
What is a microsatellite?
A short DNA sequence repeated many times in a row
In techniques described as sequencing-by-synthesis, nucleotides incorporated into the DNA strand being sequenced are identified.
As they are added to the growing DNA strand
The source DNA of BAC is
Bacterial F factors
The source DNA of PAC is
Bacteriophage DNA
Identification of microorganisms that can break down pollutants could benefit
Bioremediation
Discovery of genes involved in the synthesis of helpful chemicals could benefit
Biotechnology
In a FISH experiment, what molecule is incorporated into the single-stranded DNA probes that later allows for detection of the probes?
Biotin
How can eukaryotic chromosomes be distinguished from one another in the microscope?
Chromosomes can be treated with dyes that produce unique banding patters for each chromosome
What process is used for physical mapping?
Cloning fragments of chromosomal DNA
What is subcloning?
Cloning of a small DNA fragment from a larger DNA fragment that was previously cloned
The approach that uses information from genome projects to understand genetic variation among different populations is called ______________ genomics.
Comparative
A complete physical map of the human genome requires one ____________ for each autosome, and the X chromosome, and the Y chromosome
Contig
What does the term mapping mean in the context of genetics?
Determining the locations of genes along individual chromosomes
Metagenomics is aimed at
Determining the sequence of DNA fragments in environmental samples
Comparative genomics is the study of genetic variation among
Different species
What model organisms had their genomes sequence as part of the Human Genome Project?
Drosophila melanogaster (fruit fly) Escherichia coli (a bacterium Arabidopsis thaliana Mus musculus (mouse)
The first individual in which a disease-causing allele occurred is called a
Founder
How the components of a genome interact to produce the traits of an organism is studied in _____________ genomics
Functional
The study of the interactions of many genes and the ways that the components of a genome interact to produce an organism's traits is called _______________ genomics
Functional
A genome-sequencing project helps basic research scientists by providing information about which ________ a given species had
Genes
A diagram that shows the relative locations of genes or other DNA segments along a chromosome is called a _______________ map
Genetic
The location of a fluorescently-labeled probe relative to the banding pattern of a chromosome is determined by comparing the results of a FISH experiment to a sample of chromosomes that have been stained with ___________
Giemsa
What is studied in functional genomics?
How the components of a genome interact to produce the traits of an organism
Characterization of microorganisms that reside in the human body could benefit
Human medicine
In positional cloning, a gene is cloned based on
Its location along a chromosome
A microsatellite can be amplified by PCR using primers that are located
On both sides of the microsatellite
In pyrosequencing, the incorporation of a nucleotide into a growing DNA strand in monitored by measuring the release of
PPi
The locations of several probes labeled with different fluorescent molecule scan be determined simultaneously in a type of FISH experiment called chromosome
Painting
Which mapping strategy involves cloning many pieces of DNA and characterizing their sizes and relative locations along a chromosome?
Physical mapping
The strategy in which a gene is cloned based on its mapped location along a chromosome is called _____________ cloning
Positional
Chromosomal walking is a method of __________ in which researcher begins at a specific site on a chromosome and analyzes __________ until the gene of interest is reached.
Positional cloning, a series of subclones
To analyze a microsatelliete sequence, a scientist must first amplify it by PCR. This requires the use of PCR _____________ that flank the repeated sequence in the microsatellite.
Primers
What is an example of a molecular marker?
RFLP, Microsatellite, Single-nucleotide polymorphism
The sequencing method that monitors DNA synthesis in real time is called
Sequencing by synthesis
Why have agriculturally important species been selected for genome sequencing?
Understanding their genomes may aid in the development of new strains with improved traits.
The Global Ocean project sampled ocean water in numberous locations in the Pacific Ocean and sequenced DNA obtained from these samples. When the researchers analyzed the DNA sequences, they found that most of the DNA sequences
Were unique
A vector that can carry a large fragment of chromosomal DNA is a
YAC, BAC, or PAC
The technique of fluorescence in situ hybridization involves the use of ________ that hybridizes to a __________
fluorescent probe; specific site on an intact chromosome
A research endeavor with the goal of determining the sequence of DNA bases in an organism's entire genome is called a _____________ - ___________ project
genome-sequencing
Identifying overlapping cloned DNA fragments in a DNA library and determining the relative order of those fragments is a goal of _________ mapping
physical
To map the distance between molecular markers via testcrosses, the markers must be
polymorphic
During in situ hybridization, a DNA probe made from a cloned gene binds to
Its complementary sequence on a particular chromosome
A collection of recombinant vectors that each contain a particular fragment of chromosomal DNA is called a DNA ____________
Library
Identifying and localizing millions of genetic markers was an important procedure for the Human Genome Project's goal of obtaining a genetic _____________ map of the human genome.
Linkage
The mapping technique that relies on the frequency of recombinant offspring for determination of the distance between genetic loci is _____________ mapping.
Linkage
The site on a genetic map where a specific gene or other DNA sequence is found is called its _____________
Loci
Molecular markers can be used to identify the location of a disease-causing gene because they
May be located close to a disease-causing gene.
A collection of genes from an environmental sample is called a
Metagenome
The study of a complex mixture of genetic material obtained from an environmental sample is called __________
Metagenomics
In addition to the nucleus, where is genomic information stored in a human cell?
Mitochondria
A segment of DNA that is located at a specific site along a chromosome and that has properties that allow it to be uniquely identified using molecular tools is called a ______________ marker
Molecular
The methods for DNA sequencing that have largely replaced the Sanger dideoxy method are collectively referred to as ___________- generation sequencing technologies
Next
One of the methods that is used for genome-sequencing projects involves the sequencing of randomly generated DNA fragments followed by identification of overlapping regions in the DNA sequence. This method is called ______________ sequencing.
Shotgun
The term locus refers to the
Site on a genetic map where a specific gene is found.
Shotgun sequencing is a method of DNA sequencing in which
The DNA fragments to be sequenced are randomly generated from larger DNA fragments.
The cloning of a small piece of DNA that is part of the DNA insert of a larger clone is called
Subcloning
What is high-throughput sequencing?
The ability to rapidly sequence large amounts of DNA
In the field of study proteomics, researchers study
The roles of all cellular proteins in producing the traits of cell types and organisms
What is metagenomics?
The study of a complex mixture of genetic material from an environmental sample
Genomics is the molecular analysis of
The entire genetic composition of a species.
The Global Ocean Project discovered that closely related species variants called ________________ coexist in the same environment
Subtypes
A short repetitive DNA sequence, such as a dinucleotide sequence repeated many times in a row, is called a
Microsatellite
Many microorganisms that live in soil, water, and the human intestinal tract are difficult to study because: 1) Their genomes are too large to sequence using traditional methods. 2) Researchers may not understand their growth requirements. 3) They may require the presence of a complex microbial community. 4) They have very high mutation rates, which makes it difficult to keep them alive.
2&3
A DNA library is a collection of
Recombinant vectors containing various fragments of chromosomal DNA
A site that can be uniquely amplified by PCR is called a _____________ -_____________ site, or STS
Sequence-tagged