Biol 111: Lab 10 Quiz

There are 130 offspring from a cross of AaBb x AaBb. If Mendelian ratios are followed, how many offspring would you expect to have recessive phenotypes for both traits?

8

If you allow a heterozygous tall plant that is heterozygous for purple flowers to self-pollinate, how many tall and purple plants would you expect in the F2 generation if you sample 16 individuals? Both tall and purple flowers are dominant.

9

Because the Arabidopsis are transgenic (they have had genetic material added/modified), what is the proper disposal procedure?

Biohazard disposal

Based on the phenotypes of the parental plants and F1 plants from the Heredity I lab, which phenotypes are dominant?

GFP positive Trichomes

To examine the [a] plants for the [b] of interest, a dissecting scope will be used to look for presence of [c] and a [d] will be used to look for GFP.

[a] --> F2 [b] --> phenotypes [c] --> trichomes [d] --> blue light

When heterozygous tall plants with purple flowers self pollinate to give rise to tall and dwarf plants with purple flowers, it demonstrates the following:

segregation of alleles

This week's procedures will include:

~ Examining plants under the dissecting scope to look for trichomes. ~ Examining plants under blue light to look for GFP.

Which of the following are true statements about Arabidopsis?

~ It is a model organism. ~ Arabidopsis is easy to grow in a lab. ~ It is possible to create plants that carry genes not normally found in Arabidopsis.

Which statements about genetics and heredity are TRUE?

~ The genotypic and phenotypic frequencies in a cross between AaBb x AaBb are not the same. ~ Genotype refers to the alleles an individual possesses.

Based on the phenotypes of the P generation and F1 generation, what are possible phenotypes in the F2 generation?

~ Trichomes, no GFP ~ Trichomes, GFP positive ~ Glabrous, no GFP ~ Glabrous, GFP positive