biology I lab final (practice questions)
match the phase (or step) of photosynthesis to the process? a. dark reactions b. light reactions
1. 1st phase/step- b 2. 2nd phase/step- a
from start to finish, place in order the laboratory steps that must be accomplished in order to identify DNA from samples/organisms. a. PCR amplification b. gel electrophoresis of PCR products c. DNA isolation d. analysis of gas results
1. 1st step- c 2. 2nd step- a 3. 3rd step- b 4. 4th step- d
match the step of Par with what is occurring in the test tube? a. double stranded DNA melts to single stranded DNA b. DNA strand elongates with DNA polymers c. DNA primer hybridize to the DNA strand
1. Annealing- c 2. Denaturation- a 3. extension- b
match the photosystem to the specialized pigment molecules? a. P700 b. P680
1. PSI- a 2. PSII- b
match the process to its substrate a. electrons b. pyretic acid/pyruvate c. glucose
1. glycolysis- c 2. krebs cycle- b 3. ETC- a
ALP catalyzes the hydrolysis of __ attached to proteins or lipids? a. phosphate groups b. substrates c. phenol groups d. methyl groups
a
CO2 is converted into a stable organic compound through a process known as? a. carbon fixation b. nitrogen fixation c. oxygen fixation d. chemiosmosis
a
DNA from Bacillus thuringiensis is incorporated into the genomes of crops to make them? a. pest resistent b. herbicide resistent c. drought resistant d. fungi resistant
a
__ is more resistant than __ to processing and can be extracted from highly processed foods, making it a Bette option for identifying GM in food a. DNA, proteins b. DNA, bacteria c. bacteria, proteins d. proteins, bacteria
a
a molecules weight ruler, aka ladder, is used as a reference to determine the charge (i.s. negative vs. positive) of your products. a. true b. false
a
being able to reproduce the results of an experiment on a separate day follows the principles of? a. similarity of causation b. objectivity c. parsimony d. uniformity e. flexibility
a
blue-colored objects absorb which wavelengths of light the most? a. red b. none of these c. purple d. blue e. UV f. green
a
can dominant alleles be masked by recessive alleles? a. no b. yes c. only by certain recessive alleles d. I didn't read the pre-lab
a
during the dark reactions, atmospheric carbon dioxide (CO2) is brought into the lead through tiny pores called __? a. stomata b. stamen c. sepals d. stigma
a
fermentation generates energy (i.e., ATP) a. true b. false
a
how much ATP is generated by the complete oxidization of pyruvate into CO2 and H2O via aerobic respiration? a. 36-38 b. 20-30 c. 15-20 d. 30
a
in prokaryotic cells, catabolism of pyruvate takes place in the __. whereas in eukaryotic cells, it occurs in the __? a. cytoplasm, mitochondria b. fermentation, cytoplasm c. cytoplasm, NADH d. mitochondria, glycolysis
a
in the United States, genetically modified foods do not have to be labeled as such. a. true b. false
a
independent variables are usually plotted on the X-axis of a graph, whereas dependent variables are usually plotted on the Y-axis? a. ture b. false
a
large complexes of proteins and pigments (light-absorbing molecules) that are optimized to harvest light are called? a. photosystems b. photographs c. photosynthesis d. photosphere
a
many species are unable to utilize oxygen as a final electron acceptor during cellular respiration due to? a. lack of ETC proteins lack of oxygen in the environment, lack of enzymes needed to inactivate free radicals b. lack of enzymes needed to inactivate free radicals, lack of carbon in the environment, lack of ATP proteins c. lack of ATP proteins lack of oxygen in the environmental lack of enzymes needed to inactivate restrictive radicals d. lack of enzymes needed to inactivate restrictive radicals, lack of ETC proteins, lack of oxygen in the environment
a
moving molecules against their concentration gradient requires energy. a. true b. false
a
name the two types of peas that we will be working with in the cellular respiration lab? a. dormant peas and germinating peas b. green peas and snow peas c. snap peas and green peas d. green peas and germinating peas
a
plant cells are like red blood cells and can potentially burst when placed in solutions of varying tonicity. a. true b. false
a
potential allergic reactions to novel protein and antibiotic resistance from selectable markers are major arguments against/concerns associated with the GMO industry. a. true b. false
a
the first offspring produced from a monohybrid cross is called the ...? a. F1 generation b. F2 generation c. M1 generation d. Mono1
a
the movement of water molecules from a region of high free water concentration to a region of low free water concentration is known as __? a. osmosis b. proton pump c. active transport d. facilitated diffusion
a
the process by which organisms chemically oxidize organic compounds as a means of generating ATP is? a. cellular respiration b. photosynthesis c. breathing d. triphosphate pathway
a
what are autosomes? a. chromosomes 1-22 b. sex chromosome s c. pairs of chromosomes d. self-replicating chromosomes
a
what color will the product of todays experiment (pNP) turn? a. yellow b. blue c. red d. violet
a
what does homozygous mean? a. when the same pair of alleles composes a trait b. when two different alleles compare a trait c. when genes replicate d. when an allele is missing
a
what happened it either the enzyme or substrate is limited? a. the enzymes reaction rate will remain constant b. the enzymes reaction rate will increase c. the enzymes reaction rate will decrease d. the enzymes reaction rate will cease
a
what is the P generation? a. the first set of parents crossed b. the first set of offspring c. the phenotype of the generation d. the party generation
a
what is the appearance of a particular trait called? a. phenotype b. genotype c. appearance factor d. dominant allele
a
what is the chemical equation for photosynthesis? a. 6CO2 + 6H2O + light --> C6H12O6 + 6)2 b. C6H12O6 + 6O2 -->6H2O +6CO2 +ATP c. both a and b d. neither a or b
a
what is the end product of photosynthesis (for the plant)? a. glucose b. carbon dioxide c. light d. heat
a
what is the genetic composition of an organism called? a. genotype b. phenotype c. heterozygous d. homozygous
a
what is the in vitro substrate alkaline phosphate (ALP) that we will be using in lab? a. pNNP b. pNP c. Q10 d. none of these are correct
a
what is used to compare observed ratios for a given cross compare with predicted ratios? a. Chi-square analysis (x2) b. punnet square c. standard deviation d. margin of error
a
what number do chromosomes of somatic cells contain? a. diploid number (2n) b. haploid number (n) c. triploid number (3n) d. they do not contain any
a
what type of buffer is used to run gel electrophoresis? a. TAE b. Mg2+ c. TEA d. phosphate buffer
a
what voltage is gel electrophoresis run at? a. 100V b. 200V c. 100mV d. 1500MHz
a
what will happen in the experiment when light hits the chloroplasts? a. all of the above b. DPIP will be reduced c. DPIP will change from blue to colorless d. there will be an increase in light transmittance
a
what will happen when a red blood cell is place in a hypertonic solution? a. the cell becomes shriveled b. the cells becomes turgid c. the cell becomes lysed d. the cell remains normal
a
when an enzymes reaction rate doubles for every 20 degree celsius increase in temperature its called the Q10 effect. a. true b. false
a
when lead pigments absorb light, electrons are boosted to a higher energy level, providing energy to? a. all of the above b. produce ATP c. reduce NADP+ d. none of the above
a
which has a smaller wavelength? a. electrons b. visible light
a
which of the following molecules can diffuse through a cell membrane? mark all that apply. a. small molecules b. charged molecules c. large molecules
a
who is known as the 'founder' of genetics? a. gregor mendel b. Charles Darwin c. jimmy neutron d. Thomas hunt Morgan
a
you must heat up the agarose to dissolve it. a. true b. false
a
your heart rate, or pulse, is the number of times your heart __? a. beats per minute b. beats for love c. beats per second d. beats by Dr. Dre
a
at which stages of aerobic cellular respiration are ATP produced? check all that apply a. glycolysis b. kerb's cycyle c. ETC d. chemoiosmosis
a, b, d
what factors determine how fast water moves across a membrane? select all that apply a. solute concentration difference b. the size of the cell c. hydrostatic pressure
a, c
if you were to attend a lab in person, what is the proper attire you must wear before entering the lab? (select all the apply) a. lab coat b. shorts c. long pants d. ripped jeans e. mask f. flip flops g. closed toe shoes
a, c, e, f
what determines resolution? check all that apply a. visual acuity b. stage size c. number of lenses d. lense color e. magnification f. lense diameter g. lens quality h. number of oculars
a, e, f, g
DNA polymerase is derived from a bacterium that lived in? a. methane pockets b. high-temperature steam vents c. hypersaline retention pods d. hydrothermal vents
b
How long is gel electrophoresis typically run for? a. 3 hours b. 30 minutes c. 30 seconds
b
a denatured enzyme can catalyze a chemical reaction. a. true b. false
b
a scanning electron microscopes (SEM) does what of the following? a. produce an image when a beam of electrons are passes through a sample b. produce an image that is upside down c. produce 3-D images of the surfaces of objects d. produce an image that is smaller than the original
b
aerobic respiration yield more ATP than anaerobic respiration. a. true b. false
b
dissecting microscopes allow higher magnification than compound microscopes. a. true b. false
b
during the process of gel electrophoresis, combs are used to? a. preserve the structure of DNA b. form the wells that samples will be placed in c. immobilize the gel for the duration of electrophoresis d. conduct an electrical current through the gel
b
enzyme activity typically occurs within a very broad pH range. a. true b. false
b
gel electrophoresis separates DNA on the basis of what? a. single-stranded vs. double-stranded DNA b. size of DNA c. charge of DNA d. none of these options are correct
b
if the results of a patients two "random" glucose readings are 196 mg/dL and 200 mg/dL, this person meets the criteria of diabetes mellitus. a. true b. false
b
in order to separate PCR product bands produced by plant and GMO primers, a __ agarose gel is required. a. 1% b. 3% c. 6% d. 12%
b
only glucose can be broken down to generate ATP. a. true b. false
b
photosynthesis is a catabolic process. a. true b. false
b
simple diffusion requires energy input. a. true b. false
b
the variable that is manipulated or varied by the researcher in an experiment is the? a. dependent variable b. independent variable c. codependent variable d. non-dependent variable
b
to view a specimen under an electron microscope, it has to be living. a. true b. false
b
what color-producing chemical will be used in todays lab to determine the concentration of glucose solution through absorbance readings? a. copper (I) oxide b. chromatin c. carbonate d. chlorophyll a
b
what is the enzyme we will be using in lab today? a. PAL b. ALP c. pNPP d. pNP
b
what is the name of the machine we will use in todays lab to measure the amount of light that is absorbed by a solution? a. absorbometer b. spectrophotometer c. micropipettes d. photometer
b
what volume of liquid a P200 micropipette at the setting (155) measure out? a. 155uL b. 15.5uL c. 155mL d. 310uL e. 310mL
b
what will happen when a red blood cell is place in distilled water? a. creation (shrinking of the cell) will occur b. Lysis will occur c. plasmolysis will occur d. the cell will become turgid
b
the act of placing genes for selected traits directly into crop plants is a form of genetic modification known as___? a. genetic inbreeding b. genetic transformation c. cross manipulation d. magic
c
the cause behind the results of an experiment will stay the same if we repeat the experiment tomorrow follows the principle of? a. similarity of causation b. parsimony c. uniformity d. objectivity e. flexibility
c
what is added to a gel that allows us to visualize DNA? a. buffer b. methylene blue dye c. sybersafe dye d. agarose
c
what macromolecule is an enzyme classified as? a. carbohydrate b. lipid c. protein d. nucleic acid
c
what micropipette(s) could be used to measure out 175uL of liquid? a. P1000 b. P20 c. P200 d. P100
c
100uL is the equivalent to? a. 100mL b. 1000mL c. 0.001mL d. 0.1mL
d
if you were given a sample of bacteria to analyze, which microscopes would be best for quickly identifying the specimen? a. slide viewing b. electron c. dissecting d. compound
d
in an experiment designed to determine the rate of reaction bases on the content of each beaker, the hypothesis that states there will be no differences in the outcome of the experiment is? a. zero hypothesis b. alternative hypothesis c. differential hypothesis d. null hypothesis
d
primers serve as __ for amplifying the target region of the DNA to be copied. a. catalysts b. midpoints c. inhibitors d. start and stop points
d
the liner relationship between the concentration of a colored solution and its light absorbance is known as the __? a. Kirchhoff's law b. planck radiation law c. Wien's displacement law d. beer-lambert law
d
there is a(n) __ relationship between the color of a solution and the wavelength of light it best absorbs. a. synergistic b. inverse c. proportional d. linear
d
tonicity is the measure of what? a. air pressure b. solute concentration c. ion concentration d. osmotic pressure
d
what is the correct order for the steps of PCR amplification? a. extension, denaturation, annealing b. annealing, denaturation, extension c. annealing, extension, denaturation d. denaturation, annealing, extension
d
what is the optimal temperature for most enzymes? a. 60+ degrees Fahrenheit b. 40 to 60 degrees Fahrenheit c. 20 to 40 degrees Fahrenheit. d. 20 to 40 degrees celsius e. 40 to 60 degrees celsius f. 60+ degrees celsius
d
what is the test that canoe used to detect proteins that are produced in GM crops? a. protonation analysis b. InstaGene c.DNA sequencing d. ELISA
d
put the scientific method steps in the correct order a. analyze results b. design an experiment c. repeat d. making observations e. developing hypothesis
d, e, b, a, c
ocular lenses found in most compound light microscopes a magnification of..? a. 100x b. 1000x c. 1x d. 0.5x e. 10x
e
your resting heart rate is __? a. your heart rate after exercise b. is when your heart is pumping the least amount of blood c. when your heart rate is at its slowest d. when your heart rate is pumping the most amount of blood e. B and C f. A and D
e
