Biology Lab Exam - Microscope and Calculations

How do you calculate magnification on a dissecting microscope on low, medium and high?

(Ocular Lens) x (Magnification Knob) Low: 10x0.8 = 8x Med: 10x2 = 20x High: 10x4 = 40x

How do you calculate the magnification on a compound microscope on low, medium, and high?

(Ocular Lens) x (Objective Lens) Low: 10x4 = 40x Med: 10x10 = 100x High: 10x40 = 400x

How do you calculate the actual size of an organism *on low power*? (40x)

Actual size = field of view/# of organisms that fit across field of view Example: 5mm/2 organisms = 2.5mm (2500 micrometers)

Between the two microscopes, which has greater *magnification*?

Compound

Between the two microscopes, which has greater *resolution*?

Compound

How do you calculate drawing magnification?

DM = Size of Drawing/Actual Size Example: 50mm/2.5mm = 20x drawing magnification

Between the two microscopes, which has greater *Depth of Field*?

Dissecting

Between the two microscopes, which has greater *Size of Field of View*?

Dissecting

What is the field of view on compound microscope? Low, Med, High:

Low: 40x ~ 5mm (5000 micrometers) Med: 100x ~ 2mm (2000 micrometers) High: 400x ~ 0.5mm (500 micrometers)