Biology Practical 1

Identify these blood types.

0 A B AB

Measure the length of this object. Give your answer as follows: Millimeters = Centimeters = Meters= Kilometers=

141 mm 14. 1cm 0.141 m 0.000141 km

Identify the following parts of the microscope: 22.-32.

22. Ocular lenses or eyepieces 23. Arm 24. Objective lenses 25. Nosepiece 26. Stage 27. Course Adjustment Knob 28. Fine Adjustment Knob 29. Base 30. Light source 31. Condenser 32. Stage control knobs

Look at this graph showing the absorption spectrum of Chlorophyll b. 51. Which wavelengths of light are most captured by chlorophyll b? 52. Which wavelengths of light are least captured by chlorophyll b?

51. 460 and 660 nm 52. 700-720 nm

Identify each piece of lab equipment by name. 53-66

53. Pipette 54. Pipette pump 55. Stirring rod 56. Scoopula 57. Beaker tongs 58. Thermometer 59. Test tube 60. Test tube clamp 61. Graduated cylinder 62. Beaker 63. Erlenmeyer flask 64. Crucible tongs 65. Petri dish 66. Watch glass

Imagine that the lab technician preparing the F2 corn cobs you used in lab performed the wrong cross! He crossed a corn plant, homozygous for purple color and heterozygous for smooth shape, with another corn plant that was yellow and wrinkled. Purple color is dominant over yellow Smooth shape is dominant over wrinkled. Predict the phenotypic ratio of the offspring made through this technician's error. Show all of your work.

8 Purple smooth: 8 Purple wrinkled (or 1:1)

These tubes contain yeast, molasses, and water. Assuming that both tubes started with air bubbles of the same size, which do you think was incubated at room temperature and which was incubated at 0°C? Why? What gas is being produced? What process produces the gas bubbles in these samples?

A was room temperature and B was at 0°C because Tube A has a bigger bubble. This indicates that more gas was produced in Tube A. Tube A had a higher respiration rate because molecular motion is slowed at 0°C. CO2 Respiration

Look at the following karyotypes (A and B) and determine the sex of each individual and whether there are chromosomal abnormalities.

A. Female. Abnormal - trisomy 8 B. Male - Normal

Look at the following pictures of cells. The cell on the left (A) is in an isotonic solution. Indicate whether the cell is in a hypotonic, isotonic, or hypertonic solution in pictures B and C. Picture B = Picture C =

B is in a Hypotonic solution C is in a Hypertonic solution

Which of the solutions in the agar gel has the heaviest molecular weight? Which has the lightest molecular weight? How can you tell?

Blue Red/Purple The heavier the molecular weight, the slower it moves through the agar gel. The heaviest molecules will not move as far.

A tube contains enzyme and its substrate What effect would boiling have on the reaction rate? Why? What effect would raising the temperature slightly have on the reaction rate? Why? .What effect would reducing the temperature have on reaction rate? Why? Do all enzymes function best at the same temperatures? Explain.

Decrease the reaction rate because boiling denatures most enzymes. Increase the reaction rate because increased temperature increases molecular motion up to the optimal temperature Decrease the reaction rate because decreased temperature reduces molecular motion. No. Organisms adapted to particular environmental conditions have enzymes that work well in those conditions.

Simba has blood type O. Mufasa and Serabi are Simba's parents. Mufasa has blood type B and Serabi has blood type A. Using a Punnett Square, show how these two parents produced a child with Blood Type O. Note: In reality, cats do not have the same blood tvpes as humans.

Mufasa must be BO (|®;) and Serabi must be AO (IAj) to produce a child 00 (il).

Which part of this leaf has the most starch: the patched part or the unpatched part? Why didn't this plant produce starch evenly across the leaf?

The unpatched part has more starch This is evident because the iodine didn't react with starch where the patch was placed. The patch prevented light from reaching this portion of the leaf, blocking the photosystems. The plant cannot make starch where it doesn't photosynthesize.

What types of lab equipment would you use to measure the following: Temperature of a solution Volume of a liquid Weight of a solid object Length of your finger

Thermometer Graduated cylinder, beaker, Erlenmeyer flask, pipette (Do you know which is the most accurate? Triple beam balance, hanging pan balance, digital scale, scale . Metric ruler

A polar solvent was used as the chromatography solution to separate these plant pigments. Which pigment (color) is the most polar? Justify your answer to #19. How do you know which pigment is the most polar? Would our answer to #19 change if the strip was placed in a nonpolar solvent? If so, how?

Yellow In a polar solvent, the most polar pigment would rise the highest. Yes. In a nonpolar solvent, the most polar pigment would be the lowest.