BIOS 2114 chapter 13-14 clicker
(A. The ATP must be transported across the inner mitochondrial membrane, after which it freely diffuses into the cytosol through the permeable outer membrane. Embedded in the inner membrane are dedicated ADP/ATP antiporters that serve the dual purpose of exporting ATP and bringing in new ADP, which can then be converted into ATP during oxidative phosphorylation. B. During oxidative phosphorylation, the NADH and FADH2 generated by the citric acid cycle donate their electrons to an electron-transport chain in the inner mitochondrial membrane. As the electrons move along this chain, the energy released is used to drive protons across the inner mitochondrial membrane. This movement of protons produces a proton gradient across the membrane, which then serves as a source of energy for the generation of ATP. If the inner mitochondrial membrane were made "leaky," the proton gradient would dissipate. Thus although acetyl CoA would continue to be oxidized by the citric acid cycle, and electrons donated to the electron-transport chain, these processes could no longer promote the production of ATP. C. Compartmentalization provides a basic mechanism for the regulation of independent sets of reactions, including the citric acid cycle, fatty acid oxidation, glycolysis, and gluconeogenesis. In some cases, metabolic reactions are physically compartmentalized to separate anabolic from catabolic reactions. For example, in the mitochondrial matrix, oxaloacetate is used in the citric acid cycle to help oxidize the acetyl carbons of acetyl CoA. However, oxaloacetate in the cytosol tends to be consumed by biosynthetic enzymes that use the molecule as a precursor for the production of amino acids such as aspartate. By keeping these reactions separate, the cell can control whether a molecule is used in an anabolic or catabolic reaction. A second advantage of compartmentalization is the co-localization and concentration of enzymes with their substrates, which can enhance reaction rates.)
Although the outer mitochondrial membrane is permeable to all small molecules, the inner mitochondrial membrane is essentially impermeable in the absence of specific transport proteins. Consider this information and what you have learned about the citric acid cycle to address the following questions. A. The ATP generated by oxidative respiration is used throughout the cell. The majority of ATP production occurs in the mitochondrial matrix. How do you think ATP is made accessible to enzymes in the cytosol and other organelles? B. If the inner mitochondrial membrane were rendered as permeable as the outer membrane, how would that affect oxidative phosphorylation? Which specific processes would stop and which remain? C. Present two types of benefits derived from separating the reactions of glycolysis in the cytosol from those that occur during the citric acid cycle in the mitochondrion.
(A. NADH → NAD+. B. Under anaerobic conditions, it is the only means of regenerating the NAD+ required for glycolysis, the main energy-generating pathway of an anaerobically growing yeast cell.)
Anaerobically growing yeast further metabolizes the pyruvate produced by glycolysis to CO2 and ethanol as part of a series of fermentation reactions. A. What other important reaction occurs during this fermentation step? B. Why is this reaction (that is, the answer to part A) essential for the anaerobically growing cell?
(NADH is an activated carrier molecule used as a cofactor for many enzymes that catalyze redox reactions. NADH also donates electrons to the electron-transport chain, which is essential for the production of ATP.)
Although ATP and NADH are both important activated carrier molecules, ATP hydrolysis provides the direct molecular energy for most biochemical reactions. Why do the mitochondria also need to generate high levels of NADH?
TRUE
*TRUE OR FALSE* (if a statement is false, explain why it is false) A. CO2 and H2O are generated during the oxidation of food molecules.
TRUE
*TRUE OR FALSE* (if a statement is false, explain why it is false) Amino acids can be transported into the mitochondria and converted into acetyl CoA.
TRUE
*TRUE OR FALSE* (if a statement is false, explain why it is false) Anaerobic respiration is not the same as fermentation, as only the former requires an electron-transport chain.
FALSE (Activated carriers have high-energy bonds that can drive other reactions when broken. Heat may be released during these reactions and may increase the reaction rates, but is not a form of energy that is stored in biological systems.)
*TRUE OR FALSE* (if a statement is false, explain why it is false) B. Activated carrier molecules store heat energy for the cell to use later.
FALSE (False. Catabolism comprises the metabolic reactions that are involved in breaking large molecules into smaller molecules. Anabolism encompasses the reverse types of reactions: synthesizing larger molecules from smaller molecules.)
*TRUE OR FALSE* (if a statement is false, explain why it is false) C. Catabolism is a general term that refers to the processes by which large molecules are synthesized from smaller molecules.
TRUE
*TRUE OR FALSE* (if a statement is false, explain why it is false) D. The oxidation of sugar is an energetically favorable process.
FALSE (At the end of a series of the 10 different reactions involved in glycolysis, the final products are two molecules of pyruvate. Pyruvate will later be broken down into CO2 and H2O in the citric acid cycle.)
*TRUE OR FALSE* (if a statement is false, explain why it is false) During glycolysis, glucose molecules are broken down to yield CO2 and H2O.
FALSE (Gluconeogenesis can begin with pyruvate as a building block to make glucose, but there are three reactions in glycolysis that are irreversible because of a large free-energy barrier. Alternative enzymes and reaction pathways are used to bypass this problem, and they require the input of energy in the form of ATP and GTP.)
*TRUE OR FALSE* (if a statement is false, explain why it is false) Gluconeogenesis is a linear reaction pathway that the cell employs to generate glucose from pyruvate and is exactly the reverse of the reactions in the glycolytic pathway.
FALSE (When glycogen phosphorylase cleaves a glucose monomer from glycogen, the product is glucose 1-phosphate. Before it can be used in glycolysis, it needs to be isomerized to glucose 6-phosphate.)
*TRUE OR FALSE* (if a statement is false, explain why it is false) Glycogen phosphorylase cleaves glucose monomers from the glycogen polymer, phosphorylating them at the same time so that they can be fed unchanged into the glycolytic pathway.
FALSE (When fructose 1,6-bisphosphate is cleaved, the products are dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Only after the subsequent isomerization of dihydroxyacetone phosphate is the second molecule of glyceraldehyde 3-phosphate produced.)
*TRUE OR FALSE* (if a statement is false, explain why it is false) The cleavage of fructose 1,6-bisphosphate yields two molecules of glyceraldehyde 3-phosphate.
FALSE (Although the proteins of the electron-transport chain collect electrons from the NADH and FADH2 cofactors, these high-energy electrons go through a series of transfers along the electron-transport chain. The energy released with each transfer moves protons across the inner mitochondrial membrane. It is this proton gradient that provides the energy to synthesize ATP.)
*TRUE OR FALSE* (if a statement is false, explain why it is false) The proteins of the electron-transport chain remove a pair of high-energy electrons from the cofactors NADH and FADH2, after which the electrons move across the inner mitochondrial membrane to maintain the voltage gradient.
TRUE
*TRUE OR FALSE* (if a statement is false, explain why it is false) The pyruvate dehydrogenase complex catalyzes three different, but linked, enzymatic reactions.
FALSE (Under anaerobic conditions, mammalian cells convert pyruvate to lactate in a fermentation process. The lactate is subsequently excreted from the cell.)
*TRUE OR FALSE* (if a statement is false, explain why it is false) When subjected to anaerobic conditions, glycolysis in mammalian cells continues and causes a buildup of pyruvate in the cytosol.
TRUE
*TRUE OR FALSE* (if a statement is false, explain why it is false) With respect to the amount of energy stored in molecules of the body, 6 g of glycogen is the equivalent of 1 g of fat.
A carbon atom in a CO2 molecule in the atmosphere eventually becomes a part of one of the enzymes that catalyzes glycolysis in one of your cells. The CO2 first enters a cell in a corn leaf, where photosynthesis fixes the carbon to make it part of a sugar molecule; this travels from the leaf to an ear of corn, where it is stored as part of a polysaccharide *starch* molecule in the corn seed. You then eat a corn chip made from the corn seed. You digest the corn seed, and the free *glucose* travels in your bloodstream, eventually being taken up by a liver cell and stored as *glycogen*. When required, this storage molecule breaks down into glucose 1-phosphate, which enters the glycolytic pathway. Glycolysis produces *pyruvate*, which is converted into acetyl CoA, which enters the *citric acid cycle*. Several intermediates in this process can provide the carbon skeleton for the production of *amino acids*, which are then incorporated into the enzymes that catalyze steps in glycolysis.
A carbon atom in a CO2 molecule in the atmosphere eventually becomes a part of one of the enzymes that catalyzes glycolysis in one of your cells. The CO2 first enters a cell in a corn leaf, where photosynthesis fixes the carbon to make it part of a sugar molecule; this travels from the leaf to an ear of corn, where it is stored as part of a polysaccharide __________________ molecule in the corn seed. You then eat a corn chip made from the corn seed. You digest the corn seed, and the free __________________ travels in your bloodstream, eventually being taken up by a liver cell and stored as __________________. When required, this storage molecule breaks down into glucose 1-phosphate, which enters the glycolytic pathway. Glycolysis produces __________________, which is converted into acetyl CoA, which enters the __________________. Several intermediates in this process can provide the carbon skeleton for the production of __________________, which are then incorporated into the enzymes that catalyze steps in glycolysis. *amino acids insulin carbon fixation lactate citric acid cycle nucleotides fatty acid oxidative phosphorylation fermentation pyruvate galactose starch glucose triacylglycerol*
(A. In oxidative phosphorylation, molecular oxygen is required to produce ATP, by means of a multistage, chemiosmotic process (see Chapter 14), while in substrate-level phosphorylation, the energy released by the enzyme-catalyzed oxidation of a substrate generates ATP directly. B. Glycolysis uses substrate-level phosphorylation and generates two ATP molecules for each glucose molecule oxidized to pyruvate molecules (four total ATPs, but two of these are hydrolyzed in the first few steps of the pathway). C. Oxidative phosphorylation takes place in mitochondria as part of a multistage oxidation process. First, the citric acid cycle generates NADH and FADH2, which donate their high-energy electrons to the electron-transport chain. These electrons ultimately reduce molecular oxygen to water, and the energy of their oxidation is used to pump protons across a membrane. The proton gradient thereby produced is in turn harnessed by the enzyme ATP synthase to drive the production of ATP from ADP and inorganic phosphate. )
A. How does the generation of ATP by oxidative phosphorylation differ from ATP generation by substrate-level phosphorylation? B. What catabolic process uses substrate-level phosphorylation, and how many ATP molecules are generated in this way in the reaction pathway? C. Where does oxidative phosphorylation take place, and what other processes are required for this to occur?
(More ATP is produced from fat catabolism than from glucose catabolism. Most ATP is generated in the mitochondria, and the amount depends on the production of the NADH and FADH2 cofactors in the Krebs cycle. The Krebs cycle relies on the input of acetyl CoA. Each glucose molecule can be converted into two acetyl CoA molecules. A molecule of fat will have three fatty acid chains, with an average length of 12-16 carbons. Even if we assume very short fatty acid chains of six carbons each (the length of a glucose molecule), this would mean the production of three acetyl CoA molecules per chain, and nine total for the triacylglycerol.)
Do you expect the cell to produce more ATP from one glucose molecule or from one fatty acid molecule? Explain your answer.
(b) CH3-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-COOH (This fatty acid has 10 carbons [as opposed to 8 in (d)] and has a completely saturated hydrocarbon tail [options (a) and (c) are monounsaturated and diunsaturated, respectively). The total number of carbons determines how many acetyl CoA molecules can be derived from it; in this case it is 5. The fact that all hydrocarbon bonds are saturated means that an FADH2 molecule will be generated in the first step of the fatty acid cycle, converting a carbon-carbon single bond into a carbon-carbon double bond. If that bond is already reduced, the cycle begins at the second step. As a result, FADH2 is not generated in that cycle, lowering the total number of cofactors that can be used by the electron-transport chain to generate a proton gradient.)
Fatty acids can easily be used to generate energy for the cell. Which of the following fatty acids will yield more energy? Explain your answer. (a) CH3-CH2-CH2-CH2-CH2-CH2-CH2-CH=CH-COOH (b) CH3-CH2-CH2-CH2-CH2-CH2-CH2-CH2-CH2-COOH (c) CH3-CH=CH-CH2-CH2-CH2-CH2-CH=CH-COOH (d) CH3-CH2-CH2-CH2-CH2-CH2-CH2-COOH
b. matrix to intermembrane space
In mitochondrial electron transport, protons are pumped from the ________ a. intermembrane space to matrix b. matrix to intermembrane space c. cytosol to intermembrane space d. cytosol to matrix e. inner membrane to outer membrane
(a) simple sugars
Foods are broken down into simple molecular subunits for distribution and use throughout the body. Which type of simple subunits, listed below, is used preferentially as an energy source? (a) simple sugars (b) proteins (c) free fatty acids (d) glycerol
(c) NAD+
Glyceraldehyde 3-phosphate dehydrogenase operates by stripping a hydride ion from its substrate. Which molecule is the recipient of the proton and two electrons during this transfer? (a) oxygen (b) acetyl CoA (c) NAD+ (d) FADH
(Primarily, what is seen is that the citric acid cycle occurs more rapidly after the addition of any one of the intermediates. This means that if one intermediate is added, levels of all of them increase. In glycolysis, the intermediates downstream of the intermediate being added will be affected.)
Glycolysis and the citric acid cycle comprise two different sets of oxidation reactions. The reaction sequence for glycolysis is linear, whereas the reaction sequence for the citirc acid cycle forms a circle. How does this difference in the arrangement of reactions influence the rate of these processes when an excess amount of a single intermediate is added?
(c) 2 ATP, 2 NADH (Although 4 ATP and 2 NADH are produced, 2 ATP are converted to ADP in the early steps of glycolysis. In contrast, NADH is not converted to NAD+ in any of the reactions.)
Glycolysis generates more stored energy than it expends. What is the net number of activated carrier molecules produced in this process (number and type of molecules produced minus the number of those molecules used as input)? (a) 6 ATP, 2 NADH (b) 4 ATP, 4 NADH (c) 2 ATP, 2 NADH (d) 4 ATP, 2 NADH
(a) no oxygen is required (Glycolysis takes place in the cytosol, and although oxidation of glucose is taking place, no molecular oxygen is used.)
Glycolysis is an anaerobic process used to catabolize glucose. What does it mean for this process to be anaerobic? (a) no oxygen is required (b) no oxidation occurs (c) it takes place in the lysosome (d) glucose is broken down by the addition of electrons
(c) lactate only.
In anaerobic conditions, skeletal muscle produces _____________. (a) lactate and CO2. (b) ethanol and CO2. (c) lactate only. (d) ethanol only.
(b) it can be utilized to produce ATP under anaerobic conditions, whereas fat cannot. (Choice (b) is the answer. The breakdown of glycogen to glucose does not require oxygen; the glucose can then enter glycolysis and generate ATP by a fermentation process that produces lactic acid. In contrast, fats are broken down to acetyl CoA; this must enter the citric acid cycle, which requires oxygen to keep turning. Choice (a) is incorrect, because 1 g of glycogen (wet or dry) produces less energy than 1 g of fat. Choice (c) is incorrect, because the water bound by glycogen is not useful in keeping the body hydrated and merely contributes to making the glycogen weigh a lot. Choice (d) is incorrect, because the actual mass of glycogen required to store the same amount of energy is sixfold greater than the amount of fat.)
In humans, glycogen is a more useful food-storage molecule than fat because _____________________. (a) a gram of glycogen produces more energy than a gram of fat. (b) it can be utilized to produce ATP under anaerobic conditions, whereas fat cannot. (c) it binds water and is therefore useful in keeping the body hydrated. (d) for the same amount of energy storage, glycogen occupies less space in a cell than does fat.
(c) a high-energy thioester bond
In step 1 of the citric acid cycle, citrate is generated by the enzyme citrate synthase. The enzyme combines the two-carbon acetyl group from acetyl CoA and the four-carbon oxaloacetate. What is the source of energy that drives this reaction forward? (a) a high-energy phosphodiester bond (b) a transfer of high-energy electrons (c) a high-energy thioester bond (d) the heat of molecular collision
(d) The reaction sequence first generates one molecule of water and then consumes one molecule of water.
In step 2 of the citric acid cycle, the enzyme aconitase generates isocitrate from citrate. Which of the following statements about this reaction is true? (a) There is a substantial free-energy difference between the reactants and products of this reaction. (b) The unbonded electrons from hydroxide ions provide energy for this reaction. (c) The aconitase enzyme functions as a mutase in this reaction. (d) The reaction sequence first generates one molecule of water and then consumes one molecule of water.
(a) aldolase
In step 4 of glycolysis, a six-carbon sugar (fructose 1,6-bisphosphate) is cleaved to produce two three-carbon molecules (dihydroxyacetone phosphate and glyceraldehyde 3-phosphate). Which enzyme catalyzes this reaction? (a) aldolase (b) phosphoglucose isomerase (c) enolase (d) triose phosphate isomerase
(a) to generate a molecule of GTP
In step 4 of the citric acid cycle, the reduction of NAD+ to NADH is coupled to the generation of CO2 and the formation of a high-energy thioester bond. The energy of the thioester bond is harnessed in step 5. What is the energy used for? (a) to generate a molecule of GTP (b) to generate a molecule of ATP (c) to generate a proton gradient (d) to generate a molecule of NADH
(c) CoA
In step 4 of the citric acid cycle, the reduction of NAD+ to NADH is coupled to the generation of CO2 and the formation of a high-energy thioester bond. Which molecule provides the sulfhydryl group necessary to form the thioester bond? (a) pyruvate (b) acetyl CoA (c) CoA (d) cysteine side chain in the catalytic pocket
(a) The ratio of ATP to ADP in the cytoplasm would fall. If the inner mitochondrial membrane became permeable to protons, the electron-transport chain would continue to oxidize NADH to NAD+, transport electrons, and pump protons, so the consumption of oxygen would not fall. However, without the energy stored in a proton gradient, there is no way of driving the synthesis of ATP.
In the final stage of the oxidation of food molecules, a gradient of protons is formed across the inner mitochondrial membrane, which is normally impermeable to protons. If cells were exposed to an agent that causes the membrane to become freely permeable to protons, which of the following effects would you expect to observe? (a) The ratio of ATP to ADP in the cytoplasm would fall. (b) NADH would build up. (c) Carbon dioxide production would cease. (d) The consumption of oxygen would fall
(b) NADH
In the final step of the citric acid cycle, oxaloacetate is regenerated through the oxidation of malate and this is coupled with the production of which other molecule? (a) FADH (b) NADH (c) GTP (d) CO2
(By co-localizing three enzyme activities in a large, layered complex, the substrates are already bound and properly positioned for rapid enzyme catalysis, and the free energy released by one reaction can be readily harnessed for the next.)
In the reaction cycle involved in the oxidation of pyruvate, what are the advantages of having three enzyme activities contained in a single large complex instead of having three smaller and physically independent enzymes?
(d) excretion of more nitrogenous (ammonia-derived) wastes than with a more (Choice (d) is the correct answer. Because ammonia is given off when amino acids are metabolized to yield energy but not when sugars and fats are metabolized, you would expect more nitrogenous waste to be excreted. Choice (c) is incorrect because amino acids can be converted into pyruvate and acetyl CoA and used to generate energy. If more amino acids are consumed than are used, the body will not store them as protein in muscle tissue but will instead store them as fat, so choices (a) and (b) are incorrect.)
On a diet consisting of nothing but protein, which of the following is the most likely outcome? (a) loss of weight because amino acids cannot be used for the synthesis of fat (b) muscle gain because the amino acids will go directly into building muscle (c) tiredness because amino acids cannot be used to generate energy (d) excretion of more nitrogenous (ammonia-derived) wastes than with a more
(b) protons, down
Osmosis describes the movement of water across a biological membrane and down its concentration gradient. In chemiosmosis, useful energy is harnessed by the cell from the movement of _______________ across the inner mitochondrial membrane into the matrix _________________ a concentration gradient. (a) ATP, against (b) protons, down (c) electrons, down (d) ADP, against
(a) anaerobic, pyruvate, ethanol
Select the best option to fill in the blanks of the following statement: Fermentation is a/an _____________________ process that converts _____________ into carbon dioxide and _____________________. (a) anaerobic, pyruvate, ethanol (b) anaerobic, lactate, ethanol (c) eukaryotic, glyceraldehyde 3-phosphate, ethanol (d) prokaryotic, lactate, propanol
(c) An enzyme that catalyzes the oxidation of a molecule by removing a hydride ion.
Several different classes of enzymes are needed for the catabolism of carbohydrates. Which of the following descriptions best matches the function of a dehydrogenase? (a) An enzyme that catalyzes the rearrangement of bonds within a single molecule. (b) An enzyme that catalyzes a change in the position of a specific chemical group within a single molecule. (c) An enzyme that catalyzes the oxidation of a molecule by removing a hydride ion. (d) An enzyme that catalyzes the addition of phosphate groups to other molecules.
Oxidative phosphorylation is a process that occurs in the *inner membrane* of mitochondria. It requires an electron-transport chain that operates on the high-energy electrons taken from the activated carrier molecules *NADH* and *FADH2* that are produced by glycolysis and the citric acid cycle. These electrons are transferred through a series of molecules, and the energy released during these transfers is used to generate a gradient of *protons*, or *H+*. Because their concentration is much *higher* outside than inside the mitochondria, the flow of *protons*, or *H+*, down the concentration gradient is energetically very favorable and can thus be coupled to the production of ATP from ADP. Thus, oxidative phosphorylation refers to the oxidation of *NADH* and *FADH2* molecules and the phosphorylation of *ADP*. Without this process, the yield of ATP from each glucose molecule would be *severely* decreased.
Oxidative phosphorylation is a process that occurs in the __________________ of mitochondria. It requires an electron-transport chain that operates on the high-energy electrons taken from the activated carrier molecules __________________ and __________________ that are produced by glycolysis and the citric acid cycle. These electrons are transferred through a series of molecules, and the energy released during these transfers is used to generate a gradient of __________________, or __________________. Because their concentration is much __________________ outside than inside the mitochondria, the flow of __________________, or __________________, down the concentration gradient is energetically very __________________ and can thus be coupled to the production of ATP from ADP. Thus, oxidative phosphorylation refers to the oxidation of __________________ and __________________ molecules and the phosphorylation of __________________. Without this process, the yield of ATP from each glucose molecule would be __________________ decreased. *ADP GTP NAD+ ATP H+ NADH cytosol higher Pi electrons inner membrane protons FADH2 lower severely favorable matrix slightly glucose moderately unfavorable*
(In the first stage of catabolism, polymers are broken down into smaller subunits in the digestive system, which stretches from the mouth to the gut. Proteins are converted to amino acids, fats to fatty acids and glycerol, and carbohydrates simple sugars, including the monosaccharide glucose. In the second stage of catabolism, these simple subunits are further broken down to generate the activated carrier acetyl CoA. Acetyl CoA is the molecular input for the third stage of metabolism. In the last stage of catabolism, the acetyl CoA is oxidized to CO2, coupled to the production of large amounts of ATP, which is used as chemical energy for the cell.)
Provide a brief description of the process by which the body derives energy from food. Include in your description the three stages of catalysis, and delineate the input and output that connect each stage.
(d) NADH (Pyruvate cannot be converted into NADH, but it can be converted into the other metabolites in one or two steps.)
Pyruvate can be converted into many other molecules by various biosynthetic and metabolic pathways, which makes it a central hub in the regulation of cellular metabolism. Which of the following molecules is not made from pyruvate? (a) oxaloacetate (b) ethanol (c) lactate (d) NADH
(c) citrate
Pyruvate is an important metabolic intermediate that can be converted into several other compounds, depending on which enzyme is catalyzing the reaction. Which of the following cannot be produced from pyruvate in a single enzyme-catalyzed reaction? (a) lactate (b) oxaloacetate (c) citrate (d) alanine
(d) 3; 60
Pyruvate must move from the cytosol into the mitochondria, where it is oxidized to form CO2 and acetyl CoA by the pyruvate dehydrogenase complex. How many different enzymes and what total number of polypeptides, respectively, are required to perform this oxidation process in the mitochondrion? (a) 1; 60 (b) 3; 3 (c) 3; 26 (d) 3; 60
(d) An enzyme that catalyzes the addition of phosphate groups to other molecules.
Several different classes of enzymes are needed for the catabolism of carbohydrates. Which of the following descriptions best matches the function of a kinase? (a) An enzyme that catalyzes the rearrangement of bonds within a single molecule. (b) An enzyme that catalyzes a change in the position of a specific chemical group within a single molecule. (c) An enzyme that catalyzes the oxidation of a molecule by removing a hydride ion. (d) An enzyme that catalyzes the addition of phosphate groups to other molecules.
(b) An enzyme that catalyzes a change in the position of a specific chemical group within a single molecule.
Several different classes of enzymes are needed for the catabolism of carbohydrates. Which of the following descriptions best matches the function of a mutase? (a) An enzyme that catalyzes the rearrangement of bonds within a single molecule. (b) An enzyme that catalyzes a change in the position of a specific chemical group within a single molecule. (c) An enzyme that catalyzes the oxidation of a molecule by removing a hydride ion. (d) An enzyme that catalyzes the addition of phosphate groups to other molecules.
(a) An enzyme that catalyzes the rearrangement of bonds within a single molecule.
Several different classes of enzymes are needed for the catabolism of carbohydrates. Which of the following descriptions best matches the function of an isomerase? (a) An enzyme that catalyzes the rearrangement of bonds within a single molecule. (b) An enzyme that catalyzes a change in the position of a specific chemical group within a single molecule. (c) An enzyme that catalyzes the oxidation of a molecule by removing a hydride ion. (d) An enzyme that catalyzes the addition of phosphate groups to other molecules.
(d) ATP (When ATP levels are high, the cell does not need to break down more glucose to generate ATP. Thus, with ATP acting as an allosteric inhibitor of a key glycolytic step, there is a rapid on-off switch for this pathway.)
Step 3 in glycolysis requires the activity of phosphofructokinase to convert fructose 6-phosphate into fructose 1,6-bisphosphate. Which of the following molecules is an allosteric inhibitor of this enzyme? (a) Pi (b) AMP (c) ADP (d) ATP
(b) fumarate, FADH2
Step 6 of the citric acid cycle is catalyzed by succinate dehydrogenase. Keeping in mind that dehydrogenases catalyze redox reactions, which are the products of the reaction in which succinate is oxidized? (a) fumarate, NADH (b) fumarate, FADH2 (c) fumarate, FADH2 (d) succinyl CoA, NADH
(b) The energy derived from substrate oxidation is coupled to the conversion of ADP to ATP.
Steps 7 and 10 of glycolysis result in substrate-level phosphorylation. Which of the following best describes this process? (a) ATP is being hydrolyzed to phosphorylate the substrate. (b) The energy derived from substrate oxidation is coupled to the conversion of ADP to ATP. (c) Two successive phosphates are transferred, first to AMP, then to ADP, finally forming ATP. (d) The substrate is hydrolyzed using ATP as an energy source.
e. matrix
TCA cycle enzymes are located in the mitochondrial _____________ a. outer membrane b. inter-membrane space c. inner membrane d. cristae e. matrix
(c) energy can be extracted in usable amounts. (The amount of free energy released by glucose oxidation is the same as combustion, the amount of CO2 released is the same, and some of the energy released is still lost as heat, whatever the route.)
The advantage to the cell of the gradual oxidation of glucose during cellular respiration compared with its combustion to CO2 and H2O in a single step is that ________________. (a) more free energy is released for a given amount of glucose oxidized. (b) no energy is lost as heat. (c) energy can be extracted in usable amounts. (d) more CO2 is produced for a given amount of glucose oxidized.
(b) Acetate; CO2, NADH, and FADH2
The citric acid cycle consists of the oxidation of ____________ to produce _____________. (a) Pyruvate; CO2, NADH, and FADH2 (b) Acetate; CO2, NADH, and FADH2 (c) Pyruvate; CO2 (d) Acetate; CO2 (e) Pyruvate; NADH and FADH2
(a) Amino acids can be converted into acetyl CoA.
The citric acid cycle is a critical sequence of reactions for energy production, which take place in the matrix of the mitochondria. The reaction cycle requires materials from the cytosol to be converted into acetyl CoA, which represents the starting point of a new cycle. Which of the following statements about acetyl CoA is true? (a) Amino acids can be converted into acetyl CoA. (b) Pyruvate is converted into acetyl CoA in the cytosol. (c) Triacylglycerol molecules are transported into the mitochondrial matrix and cleaved by lipases to produce acetyl CoA. (d) Oxaloacetate is converted directly into acetyl CoA to feed the citric acid cycle.
(d) 4 (The carbons from acetyl CoA are carried through the entire first reaction cycle. In the second cycle, one of these carbons is oxidized to CO2. Two more cycles later, the second carbon molecule from that same acetyl CoA is oxidized to CO2.)
The citric acid cycle is a series of oxidation reactions that removes carbon atoms from substrates in the form of CO2. Once a molecule of acetyl CoA enters the citric acid cycle, how many complete cycles are required for both of the carbon atoms in its acetyl groupto be oxidized to CO2? (a) 1 (b) 2 (c) 3 (d) 4
(a) water
The citric acid cycle is a series of oxidation reactions that removes carbon atoms from substrates in the form of CO2. Where do the oxygen atoms in the carbon dioxide molecules come from? (a) water (b) phosphates (c) molecular oxygen (d) acetyl CoA
(b) inhibition of ATP synthesis
The concentration of H+ ions inside the mitochondrial matrix is lower than it is in the cytosol or the mitochondrial intermembrane space. What would be the immediate effect of a membrane-permeable compound that carries and releases protons into the mitochondrial matrix? (a) inhibition of the electron-transport chain (b) inhibition of ATP synthesis (c) increased import of ADP into the matrix (d) inhibition of the citric acid cycle
(d) ATP
The conversion of fructose 1,6-bisphosphate to fructose 6-phosphate is catalyzed by a fructose 1,6-bisphosphatase and is one of the final steps in gluconeogenesis. Which of the following molecules is an allosteric activator of this enzyme? (a) Pi (b) AMP (c) ADP (d) ATP
(a) It is hydrolyzed to drive the formation of ATP.
The conversion of glyceraldehyde 3-phosphate to 1,3 bisphosphoglycerate in step 6 of glycolysis generates a "high energy" phosphoanhydride bond. Which of the following best describes what happens to that bond in step 7? (a) It is hydrolyzed to drive the formation of ATP. (b) It is hydrolyzed to drive the formation of NADH. (c) It is hydrolyzed to generate pyruvate. (d) It is oxidized to CO2.
(b) pyruvate.
The final metabolite produced by glycolysis is ___________. (a) acetyl CoA. (b) pyruvate. (c) 3-phosphoglycerate. (d) glyceraldehyde 3-phosphate.
(b) The ΔG° for the oxidation of the aldehyde group on glyceraldehyde 3-phosphate to form a carboxylic acid is more negative than the ΔG° for ATP hydrolysis. (Choice (b) is the correct answer. This is another way of stating that the energetically favorable oxidation of glyceraldehyde 3-phosphate provides sufficient energy to ultimately drive the energy-requiring step of ATP synthesis from ADP. Choice (a) is untrue: NADH is an end product of the reaction glyceraldehyde 3-phosphate to 1,3-bisphosphoglycerate and therefore high (not low) levels of it would inhibit the reaction. Choice (c) is untrue: the reactions do not involve the 3-phosphate group on glyceraldehyde 3-phosphate at all. Choice (d) is untrue, because the cysteine on the enzyme is important in making a covalent intermediate with the substrate and is not oxidized by NAD+.)
The first energy-generating steps in glycolysis begin when glyceraldehyde 3-phosphate undergoes an energetically favorable reaction in which it is simultaneously oxidized and phosphorylated by the enzyme glyceraldehyde 3-phosphate dehydrogenase to form 1,3-bisphosphoglycerate, with the accompanying conversion of NAD+ to NADH. In a second energetically favorable reaction catalyzed by a second enzyme, the 1,3-bisphosphoglycerate is then converted to 3-phosphoglycerate, with the accompanying conversion of ADP to ATP. Which of the following statements is true about this reaction? (a) The reaction glyceraldehyde 3-phosphate → 1,3-bisphosphoglycerate should be inhibited when levels of NADH fall. (b) The ΔG° for the oxidation of the aldehyde group on glyceraldehyde 3-phosphate to form a carboxylic acid is more negative than the ΔG° for ATP hydrolysis. (c) The energy stored in the phosphate bond of glyceraldehyde 3-phosphate contributes to driving the reaction forward. (d) The cysteine side chain on the enzyme is oxidized by NAD+.
(c) fatty acids (In oxidative metabolism, fatty acids can only be converted to acetyl CoA, which is completely oxidized to carbon dioxide through the citric acid cycle. In addition, bacteria can use some of this acetyl CoA as a source of carbon atoms to replenish the components of the citric acid cycle, whereas animals cannot.)
The intermediates of the citric acid cycle are constantly being depleted because they are used to produce many of the amino acids needed to make proteins. The enzyme pyruvate carboxylase converts pyruvate to oxaloacetate to replenish these intermediates. Bacteria, but not animal cells, have additional enzymes that can carry out the reaction acetyl CoA + isocitrate → oxaloacetate + succinate. Which of the following compounds will not support the growth of animal cells when used as the major source of carbon in food, but will support the growth of nonphotosynthetic bacteria? (a) pyruvate (b) glucose (c) fatty acids (d) fructose
(a) malonate
The oxygen-dependent reactions required for cellular respiration were originally thought to occur in a linear pathway. By using a competitive inhibitor for one enzyme in the pathway, investigators discovered that these reactions occur in a cycle. What compound served as the inhibitor? (a) malonate (b) malate (c) fumarate (d) succinate
(d) succinate dehydrogenase
The oxygen-dependent reactions required for cellular respiration were originally thought to occur in a linear pathway. By using a competitive inhibitor for one enzyme in the pathway, investigators discovered that these reactions occur in a cycle. Which enzyme was inhibited? (a) aconitase (b) isocitrate dehydrogenase (c) malate dehydrogenase (d) succinate dehydrogenase
(b) succinate
The oxygen-dependent reactions required for cellular respiration were originally thought to occur in a linear pathway. By using a competitive inhibitor for one enzyme in the pathway, investigators discovered that these reactions occur in a cycle. Which product in the reaction pathway builds up when the inhibitor is added? (a) citrate (b) succinate (c) fumarate (d) malate
(d) NADH
The primary role of the TCA cycle in energy generation is to produce (a) Glucose (b) Pyruvate (c) ATP (d) NADH (e) Protons
(c) oxaloacetic acid cycle
The reaction cycle that uses acetyl CoA to generate electron carrier molecules needed in the electron-transport chain is important for powering the cell. Which of the names below is not one of those commonly used to describe this reaction cycle? (a) tricarboxylic acid cycle (b) Krebs cycle (c) oxaloacetic acid cycle (d) citric acid cycle
(a) It helps drive the uptake of glucose from outside the cell. (Choice (a) is correct. Choice (b) is incorrect because the phosphate transferred to the glucose is not held by a high-energy covalent bond. Choice (c) is incorrect because the reaction converts ATP to ADP, which is not useful as an energy source for most cellular reactions, even though it still has one high-energy bond. Choice (d) is incorrect because the next enzyme in the pathway is phosphoglucose isomerase, not phosphofructokinase.)
What purpose does the phosphorylation of glucose to glucose 6-phosphate by the enzyme hexokinase serve as the first step in glycolysis? (a) It helps drive the uptake of glucose from outside the cell. (b) It generates a high-energy phosphate bond. (c) It converts ATP to a more useful form. (d) It enables the glucose 6-phosphate to be recognized by phosphofructokinase, the next enzyme in the glycolytic pathway.
(a) liver
When glucose is being used up and not replaced from food intake, the blood sugar level can be maintained by synthesizing glucose from smaller molecules such as pyruvate or lactate. This process is called gluconeogenesis. Which organ is principally responsible for supplying glucose to the rest of the body when glucose reserves are low? (a) liver (b) pancreas (c) spleen (d) gall bladder
(c) mitochondrial matrix
Where are the enzymes that mediate the citric acid cycle localized? (a) cytosol (b) mitochondrial inter-membrane space (c) mitochondrial matrix (d) embedded in the inner mitochondrial membrane (e) embedded in the outer mitochondrial membrane
A
Which is the reduced form?
(c) molecules that control gene expression by base-pairing with specific mRNAs
Which of the below is the best description of microRNAs? (a) coordinately transcribed clusters of genes in bacteria (b) regulatory sequence to which transcriptional regulators bind, which can be thousands of base pairs away from the transcriptional start site (c) molecules that control gene expression by base-pairing with specific mRNAs (d) regulatory sequence that mediates binding of RNA polymerase and initiation of transcription (e) process mediated by changes in gene expression by which precursor cells become specialized cell types
(d) FADH2 and NADH
Which of the following are the reduced forms? (a) FAD and NADH (b) FADH2 and NAD+ (c) FAD and NAD+ (d) FADH2 and NADH (e) none of the above
c. CO2 is released and NADH is produced
Which of the following best describes the results of the conversion of pyruvate to acetyl-CoA prior to TCA cycle entry? a. CO2 is released only b. NADH is produced only c. CO2 is released and NADH is produced d. oxygen is consumed e. oxygen is consumed and CO2 is released
(c) Glycolysis
Which of the following breaks down glucose and produces pyruvate. (a) Citric Acid Cycle (b) Oxidative Phosphorylation (c) Glycolysis (d) Electron Transport Chain (e) Pyruvate Dehydrogenase Complex
a. acetyl co-A
Which of the following carbon compounds enters the TCA cycle directly? a. acetyl co-A b. pyruvate c. glucose d. glycerol e. ATP
(a) anaerobically growing yeast (All the other cells can perform oxidative phosphorylation to generate additional ATP.)
Which of the following cells rely exclusively on glycolysis to supply them with ATP? (a) anaerobically growing yeast (b) aerobic bacteria (c) skeletal muscle cells (d) plant cells
(b) pyruvate
Which of the following enters the mitochondrion from the cytosol for further conversion into compounds that enter the TCA cycle? (a) glucose (b) pyruvate (c) acetyl-coA (d) glyceraldehyde-3-phosphate
(d) ATP molecules are produced in the cytosol as glucose is converted into pyruvate.
Which of the following is not part of the process known as oxidative phosphorylation? (a) Molecular oxygen serves as a final electron acceptor. (b) FADH2 and NADH become oxidized as they transfer a pair of electrons to the electron-transport chain. (c) The electron carriers in the electron-transport chain toggle between reduced and oxidized states as electrons are passed along. (d) ATP molecules are produced in the cytosol as glucose is converted into pyruvate.
(b) glycogen
Which of the following polymers of glucose is used as a vehicle to store energy reserves in animal cells? (a) glucagon (b) glycogen (c) starch (d) glycerol
(d) glycogen breakdown
Which of the following processes do not take place in the mitochondria? (a) citric acid cycle (b) conversion of pyruvate to activated acetyl groups (c) oxidation of fatty acids to acetyl CoA (d) glycogen breakdown
(a) Citric Acid Cycle
Which of the following produces the most CO2 during the cellular oxidation of glucose. (a) Citric Acid Cycle (b) Oxidative Phosphorylation (c) Glycolysis (d) Electron Transport Chain (e) Pyruvate Dehydrogenase Complex
(b) Oxidative Phosphorylation
Which of the following produces the most molecules of ATP per glucose molecule. (a) Citric Acid Cycle (b) Oxidative Phosphorylation (c) Glycolysis (d) Electron Transport Chain (e) Pyruvate Dehydrogenase Complex
(d) oxidative phosphorylation (Choice (d) is the correct answer. Oxidative phosphorylation produces about 28 ATP molecules. Choice (a) produces no ATP; choice (b) nets 2 ATP; choice (c) produces 1 GTP.)
Which of the following stages in the breakdown of the piece of toast you had for breakfast generates the most ATP? (a) the digestion of starch to glucose (b) glycolysis (c) the citric acid cycle (d) oxidative phosphorylation
(c) conversion of pyruvate to acetyl CoA (Only choice (c) is correct. One carbon is oxidized and released as carbon dioxide when pyruvate is converted to acetyl CoA.)
Which of the following steps or processes in aerobic respiration include the production of carbon dioxide? (a) breakdown of glycogen (b) glycolysis (c) conversion of pyruvate to acetyl CoA (d) oxidative phosphorylation
(c) glucose 6-phosphate → fructose 6-phosphate (Choice (c) is the correct answer. The isomerase part of the enzyme name indicates that it catalyzes an isomerization reaction, and the phosphoglucose part of the name indicates the type of substrate used. The enzymes that catalyzed reactions (a), (b), and (d) would be called kinases, because they transfer phosphate groups from one molecule to another.)
Which reaction does the enzyme phosphoglucose isomerase catalyze? (a) glucose → glucose 6-phosphate (b) fructose 6-phosphate → fructose 1,6-bisphosphate (c) glucose 6-phosphate → fructose 6-phosphate (d) glucose → glucose 1-phosphate
A
Which step requires the input of energy?
(D) 10X higher in the intermembrane space
pH in matrix ~8, pH in the intermembrane space ~7 What is the difference in proton concentration? (A) 2X higher inside the matrix (B) 2X higher in the intermembrane space (C) 10X higher inside the matrix (D) 10X higher in the intermembrane space (E) 100X higher in the intermembrane space
