Chapter 12 learning curve
true
A transgenic organism is synonymous with a genetically modified organism, as both can contain recombinant DNA. true false
false
DNA polymerase is the enzyme that separates the two strands of DNA during DNA replication. true false
true
Denaturation of DNA is a necessary step in Southern blotting procedure because it separates double stranded DNA into single stranded DNA. true false
false
If a restriction site of AatII is 5′-GACGTC -3′ then 3′-GACGTC-5′ is also an AatII restriction site. true false
false
Massive parallel sequencing techniques can reliably determine the sequence of hundreds of base pairs but require more time than Sanger sequencing. true false
the lagging
Okazaki fragments are found on _____ strand of DNA. both the leading and lagging the leading the lagging the parental
false
Okazaki fragments are ligated into a continuous DNA molecule after replication is completed. true false
DNA replication, transcription, and translation
RNA is involved in which of the following cellular processes? DNA replication, transcription, and translation transcription and translation transcription DNA replication and transcription translation and DNA replication
false
Stem cells located in the gastrointestinal tract would possess more inactive telomerase compared to the amount of telomerase activity in mature neurons of the brain. true false
false
The first step of PCR is called annealing. true false
origin of replication
The point at which DNA synthesis is initiated is called the: origin of replication. replication fork. start codon. primase. primer.
false
Transformation is the process where DNA is extracted from a plasmid. true false
helicase
What is the name of the enzyme that separates the two strands of DNA during replication? topoisomerase DNA ligase DNA polymerase primase helicase
d
What would happen if telomerase stopped working in a cell in which it normally functions at high levels? a. The cells would be able to divide indefinitely. b. Cancer would be the result. c. The cell would die immediately. d. The cell would eventually die as the DNA continued to shorten.
DNA ligase
Which of the following enzymes is necessary to produce recombinant DNA? Taq polymerase topoisomerase DNA ligase helicase primase
e
Which of the following is an example of a transgenic organism? a. a patient who has received a heart valve from a pig b. a bacteria population in which a mutation conferring antibiotic resistance spontaneously arose c. a patient who has received a kidney transplant from a close relative d. a human infected with the virus Hepatitis-C who expresses viral proteins e. fish that "glow in the dark" by expressing fluorescent jellyfish proteins
b
Which of the following statements is true regarding telomeres? a. Telomeres are repaired to the same extent in germ cells and somatic cells. b. Telomeres can contain repeated base sequences. c. Telomeres contain non-essential genes that are not necessary for cell survival. d. Telomeres are enzymes that repair telomerases (which cap the end of linear chromosomes). e. Telomeres are typically only a few base pairs in size.
b
Why are primers needed for DNA replication? a. They help direct the placement of the telomeres. b. DNA polymerase can only add nucleotides to an existing chain. c. They help with the joining of Okazaki fragments. d. The primers help with the proofreading function of DNA polymerase. e. A tiny amount of RNA is needed to tell the cell where genes are located.
