Electrophoresis Quiz (lab)

If you loaded this gel picture below with positive dye samples and ran the gel in an electrophoresis chamber, where would the dye end up?

It would run towards the negative end.

If you loaded this gel picture below with negative dye samples and ran the gel in an electrophoresis chamber, where would the dye end up?

It would run towards the positive end.

Why are samples to be loaded onto an agarose gel mixed with either glycerol or sucrose?

Makes the samples more dense

What is the name of the tool used to load the samples into the wells of the agarose gel?

Micropipette

What type of buffer do we use to make our agarose gels?

TAE

What is the name of the material made from algae that we use in our lab to create the gels for electrophoresis?

agarose

The positive electrode on the electrophoresis chamber is called what?

anode

The negative electrode on the electrophoresis chamber is called what?

cathode

Agarose gel electrophoresis separates molecules based on 3 main factors. Which of the following is NOT one of the ways molecules are separated using this technique?

color

What is used to create the wells in our agarose gels?

comb

What is the role of the buffer in the electrophoresis technique?

controls ph

Which type of water is used in the preparation of an agarose gel?

deionized

In lab, what will we be applying to our agarose gels to watch their rate and direction of migration?

dye samples

If you increase the amount of voltage used to run an agarose gel the sample should run ______.

faster

Gel electrophoresis is useful for separating biomolecules. Which of the following is NOT typically separated in this manner?

lipids

How do we heat the agarose sample to dissolve it in the buffer?

microwave

The microscopic pores in the gel are called a molecular ____________.

sieve

Which type of molecule listed below will migrate the farthest (or move most quickly) within an agarose gel?

smallest