Enzymes II

A null hypothesis states that there will be a difference between the experimental treatments.

False

If a solid object is blue, then blue wavelengths are being absorbed.

False

If you follow the all the laboratory techniques and experiment instructions perfectly, one replicate for each treatment is enough for a reliable data.

False

Kyle wants to find the optimum concentration of urea fertilizer needed to obtain the maximum height of rice plants (Oryza sativa L.). How should he conduct his experiment?

Grow rice in increasing urea concentrations (0, 1, 5, 10, 20, 50 and 100 M) at a constant temperature and a constant light condition.

In the Enzyme Kinetics I and II labs, spectrometers are used to measure the absorbance of __ over time, the __ of the reaction.

benzoquinone | product

Measurements taken with a spectrometer require a __ to eliminate background readings from components other than what is being measured in the sample.

blank

For the Enzyme Kinetics I and II labs, standard curves are used to convert __ (of the product) to __ (of the product).

absorbance | concentration

When using the equation of the line for a standard curve, the __ value should be plugged in for __. Solving for __ gives the __ value.

absorbance | y | x | concentration

The volume of water required to prepare a Treatment A experimental cuvette for the Enzyme Kinetics I and II labs is 1.5 mL. To obtain this volume, the bottom of the meniscus should be brought up to the __ mark on the pipette. (Disregard the values in parentheses for the answer choices if they are confusing.)

large 8.5 mL (small 1.5 mL)

The volume of water required to prepare a blank cuvette for the Enzyme Kinetics I and II labs is 4 mL. To dispense this volume, a __ pipette should be used with a __ pi-pump.

10 mL serological | green

The volume of catecholase required to prepare a blank cuvette for the Enzyme Kinetics I and II labs is 500 µL. To dispense this volume, a __ micropipette should be used with a volume readout of __ from top to bottom.

P1000 | 0 5 0

Variables like temperature and pH can affect the rate of a reaction

True

Given the standard curve equation y = 6.0125x + 0.08 and a value of 0.235 ODU, solving for the appropriate variable gives you __ mM.

0.0258

Which of the following things do you need to do after completing the lab?

All the options are correct.

Kay wants to determine if the length of the string affects the number of times a pendulum will swing back and forth in ten seconds. What is the independent variable (IV)? Dependent variable (DV)?

IV: length of the string | DV: number of times the pendulum swings

You're designing an experiment to test the effects of blue light on flowering time in common morning glories (Ipomoea purpurea), which produce flowers that open each morning and close at night. You want to measure how long the flowers stay open on plants grown under natural light conditions versus those grown under blue light. Which of the following is the best scale of measurement for this experiment?

Record whether flowers are open every hour during the 12-hour grow light cycle

__ increase the reliability of data and assist in determining the variability of results within one experimental group

Replicates

The independent variable for the Enzyme Kinetics I and II labs is __ since it is more biologically relevant than __.

concentration of benzoquinone | absorbance of benzoquinone

The potato extract made by the instructor for the Enzyme Kinetics I and II labs is the source of __ for the reaction.

enzyme

The bottle of catechol for the Enzyme Kinetics I and II labs is the source of __ for the reaction.

substrate