enzymes (lab practical)
Which of the enzymes being tested is found in peroxisomes?
catalase
how do enzymes speed up reactions without being consumed in the process?
they lower the activation energy of a reaction
SDS can inhibit the amylase reaction by
Altering hydrophobic conditions.
what is amylase?
a digestive enzyme that breaks starch down into maltose and glucosed -released from salivary glands and pancreas
what is pepsin?
a digestive protease that breaks proteins down into peptides and amino acids
If an enzyme in solution is saturated with substrate, the most effective way to obtain a faster yield of products is to
add more of the enzyme
why does a cell only need a small amount of an enzyme?
because they can be used over and over
less amylase activity color?
dark blue
Which enzyme reaction will cause a cloudy solution to clear?
lysozyme
without enzymes...
metabolism would be wayyyy to slow!
many enzymes havve optimum activity at what pH and what body temp?
neutral & normal body temp
Alkaline phosphatase + p-nitrophenol-phosphate —> ______________
p-nitophenolate
what are enzymes?
proteins that act as catalysts for biological reactions -speed up reactions
more amylase activity color?
red orange
what does t mean when an enzyme can be denatured?
that it destroys it's activity and slows the reaction
the more amylase activity there is.....
the less starch there will be
enzymes are generally ...
very specific by acting on one substrate or one class of related substrate molecules
how can you test for amylase?
with the iodine test