Enzymes Lab Quiz

In a catalase-controlled experiment, the negative control can be shown by catalase only. a mixture of water and catalase. a mixture of hydrogen peroxide and water. a mixture of hydrogen peroxide and catalase. hydrogen peroxide only.

a mixture of hydrogen peroxide and water.

In an enzyme-controlled reaction, a substrate is the same as reactant product enzyme an inhibitor None of the answer choices are correct.

reactant

The primary function of an enzyme or any biological catalyst is to change the direction of a reaction. reduce the energy of activation and increase the rate of a reaction. reduce the energy of activation of a reaction. increase the rate of a reaction and change the direction of a reaction. increase the rate of a reaction.

reduce the energy of activation and increase the rate of a reaction.

Based on your procedure from the pH enzyme lab, how can you accurately measure how pH alone affects an enzymes ability to function? same amounts of enzymes and different pH values same amounts of enzyme and same pH values different amounts of enzyme and different pH values

same amounts of enzymes and different pH values

What happens to the amount of enzyme during a chemical reaction? decreases increases stays the same

stays the same

View the animation below, then complete the quiz to test your knowledge of the concept. Which of the following binds to the active site of an enzyme? substrate any other enzyme None of the answer choices are correct. water product

substrate

What substance decreases during a reaction and can be used to measure the activity of an enzyme? water enzyme product substrate

substrate

Increasing the concentration of enzyme at the beginning of an experiment will result in _____ substrate interacting with the enzyme. the same amount of less more

more

When testing the temperature of catalase in the enzyme lab, why did the higher temperature affect the catalase activity? Some of the catalase enzymes were denatured. Enzymatic reactions will never occur above 37 degrees Celsius. Some of the hydrogen peroxide enzymes were denatured. All of the hydrogen peroxide substrates were denatured.

Some of the catalase enzymes were denatured.

How can you determine the optimum pH of an enzyme? Test the enzyme at different pH levels. Use a pH meter. Test the enzyme in vinegar, orange juice and saliva only. Test the enzyme at the pH of 1 and pH of 14. Test the enzyme in distilled water only.

Test the enzyme at different pH levels.

An enzyme reduces the energy necessary to start a chemical reaction. This means the rate of reaction will increase. stop. remain the same. decrease.

increase.

A given enzyme works on one of two possible substrates. one type of substrate. inhibitors. any number of different substrates. no substrate.

one type of substrate

Enzymes in human cells tend to perform best at 22°C. when the person has a fever. at 98°C. at 37°C. at 60°C.

37°C

You are working in a real lab setting, and when you test the catalase and water together you see a reaction. What does this tell you? (Select all that apply.) Catalase is not specific to hydrogen peroxide. Possible contaminant in the water causing a reaction. Catalase is the negative control. You must complete the experiment again to verify your results.

Catalase is not specific to hydrogen peroxide. Possible contaminant in the water causing a reaction. Catalase is the negative control. You must complete the experiment again to verify your results.

Changing the pH will have the following effects on a catalase-controlled reaction: Increasing or decreasing the pH above or below the optimum level will decrease the activity. Decreasing the pH will always increase the enzyme activity. Increasing the pH will always increase the enzyme activity. Changing the pH will have no effect on enzyme activity Decreasing the pH will always decrease the enzyme activity.

Increasing or decreasing the pH above or below the optimum level will decrease the activity.

How does an enzyme work to catalyze a reaction? It lowers the energy of activation of a reaction. It supplies the energy to speed up a reaction. It lowers the energy barrier needed for reactants to achieve the transition state. It supplies the energy to speed up a reaction and lowers the energy barrier needed for reactants to achieve the transition state. It lowers the energy barrier needed for reactants to achieve the transition state or lowers the energy of activation of a reaction.

It lowers the energy barrier needed for reactants to achieve the transition state or lowers the energy of activation of a reaction.

How does mixing hydrogen peroxide and water test the reactivity of catalase? It shows that water and hydrogen peroxide cause a reaction. It shows that catalase causes a reaction with water. It shows that hydrogen peroxide is not the chemical causing the reaction. It shows that water is a negative control.

It shows that hydrogen peroxide is not the chemical causing the reaction.

View the animation below, then complete the quiz to test your knowledge of the concept. Enzymes are...? steroids. lipids. proteins. nucleic acids. carbohydrates.

Protein

How is enzyme activity influenced by pH? The pH changes the enzyme concentration. The pH alters the shape of the substrate. The pH can alter how the substrate fits in the active site. The pH influences temperature and therefore reaction rate.

The pH can alter how the substrate fits in the active site.

When testing the temperature of catalase, what would happen if you changed the temperature using the same tube with the same catalase and hydrogen oxide mixture? The substrate and enzyme would bind and react before all temperatures could be tested. The substrate would stop working after a few minutes before all temperatures could be tested. The enzyme would stop working after a few minutes before all temperatures could be tested.

The substrate and enzyme would bind and react before all temperatures could be tested.

Different enzymes may function better at different pH and temperature. True or False

True

Enzyme function is optimum at extremes of pH. after boiling. at very high temperatures. after cooling. at specific pH and temperature ranges.

at specific pH and temperature ranges.

Based on your results from the enzyme concentration lab, when would increasing the enzyme concentration have no effect on the reaction rate? if the majority of the enzyme has been consumed in the reaction if the majority of the substrate has been consumed in the reaction if the substrate concentration is too high if the enzyme concentration is too high

if the majority of the substrate has been consumed in the reaction

Catalase activity can be determined by measuring the amount of catalase remaining in the chemical reaction. measuring the formation of oxygen gas. measuring the formation of hydrogen peroxide. observing when the amount of hydrogen peroxide equals the amount of oxygen. measuring the ratio of catalase to oxygen.

measuring the formation of oxygen gas.

Altering the three-dimensional structure of an enzyme might prevent the substrate from binding the enzyme's active site. change the amount of energy needed for a reaction. allow the reaction to occur at a faster rate. change the type of substrate that binds the enzyme's active site. change the type of product produced in the reaction.

prevent the substrate from binding the enzyme's active site.

The two data curves on the figure illustrate that temperature ranges are not the same for all enzyme activity. temperature is not related to enzyme efficiency. pH is not related to enzyme efficiency. All of the answer choices are correct. optimal temperature ranges are not the same for all enzymes.

temperature ranges are not the same for all enzyme activity.