Exp 11: Cholesterol Lab
The image represnts which of the following chemical structure?
squalene
Which of the following is derived from a sterol?
Bile salts
Which of the following molecule transports lipids from intestine to the liver?
Chylomicrons
Formula to calculate total cholesterol or serum cholesterol (mg/dL)
(Aserum-Ablank)/(Acalibrator-Ablank) *calibrator
Total blood cholesterol
A total blood cholesterol level less than 200 mg/dl is desirable. This level of total cholesterol is associated with the least risk of heart disease.
Which compound is the source of all Carbon in cholesterol?
Acetyl CoA
Cholesterol is a 4 ringed sterol containg 27 carbons. The structure consisting of a hydroxyl group at ___ and 8-10 C side chain on ___.
C-3 C-17
LDL cholesterol
LDL cholesterol can slowly accumulated on the walls of arteries that feed blood to the heart and brain. LDL cholesterol is also known as "bad" cholesterol because a high level indicates an increased risk of heart disease. LDL cholesterol levels less than 130 mg/dl is desirable.
In ___________ cells dietary cholesterol is esterified and packaged into _____________ for transport to the ______________.
Mucosal, Chylomicrons, liver
Chylomicrons
Transport exogenous, i.e., dietary triglycerols and cholesterol from intestine to other tissues.
Lipoproteins (Classification)
according to density-chylomicrons, very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and high-density lipoprotein (HDL)
Which of the following cholesterol derivative is a polar steroid derivative?
bile salts
Convert from mg/dL to moles/L
by multiplying x 0.0259
Triglycerides
high triglyceride levels often appear with higher levels of total cholesterol and LDL cholesterol, and low levels of HDL cholesterol. Ideal triglyceride levels are considered to be less than 200 mg/dl.
What is the major component of HDL?
protein
How many Acetyl CoA are consumed in stage 1 of cholesterol biosynresis?
three
Blood test is used to measure
total blood cholesterol: 1. high density lipoprotein (HDL), 2. low density lipoprotein (LDL) cholesterol levels and triglycerides.
VLDL and LDL
transport endogenous lipids for example: synthesized in the body, triglyceol and cholesterol from liver to other tissues
HDL cholesterol
transports cholesterol back to the liver from other tissues. HDL cholesterol is also known as "good" cholesterol because a high levels seems to protect us against heart attacks by transporting cholesterol away from your arteries. On an average, men should have HDL levels ranging from 40 to 50 mg/dl, and women should have levels ranging 50 to 60 mg/dl. HDL cholesterol levels less than 35 mg/dl is considered low.
