Lab 3 Micro
What BSL category is nonpathogenic E. coli?
1
Select all of the appropriate PPE required for a BSL-1 lab. 1. lab coat 2. gloves 3. eye protection 4. Biosafety cabinet 5. Respirators
1. lab coat 2. gloves 3. eye protection
2) What are the appropriate lab procedures to handle Ebola?
Gloves, mask, eye protection, positive air flow suit, cabinets, negative air flow. All PPE
In addition to BSL-2 requirements, BSL-3 lab procedures include which of the following. Select all that apply. 1. Lab personnel might receive immunizations for microbes with which they work. 2. Access to lab is restricted and controlled at all times. 3. All work is performed in a biosafety cabinet, not just work that could create aerosolized droplets. 4. Lab personnel must change clothing before entering. 5. Lab personnel must shower upon exiting.
1. Lab personnel might receive immunizations for microbes with which they work. 2. Access to lab is restricted and controlled at all times. 3. All work is performed in a biosafety cabinet, not just work that could create aerosolized droplets.
Select all of the appropriate PPE required for a BSL-2 lab. 1. lab coat 2. gloves 3. eye protection 4. Biosafety cabinet 5. Respirators
1. lab coat 2. gloves 3. eye protection 4. Biosafety cabinet
Select all of the appropriate PPE required for a BSL-3 lab. 1. lab coat 2. gloves 3. eye protection 4. Biosafety cabinet 5. Respirators
1. lab coat 2. gloves 3. eye protection 4. Biosafety cabinet 5. Respirators
How many BSL-4 facilities are there in the US?
13
What BSL category is Staphylococcus aureus?
2
What BSL category is Mycobacterium tuberculosis?
3
BSL-3 agents are most commonly transmitted via which route? 1. vehicle transmission 2. vector transmission 3. respiratory transmission 4. fecal-oral transmission sexual transmission
3. respiratory transmission
What BSL category is Ebola?
4
What BSL category is the Ebola Virus?
4
Standard microbiological procedures--the procedures we use in Microbiology lab--include all of the following EXCEPT: 1. No eating, drinking, or applying cosmetics in the lab 2. Washing hands after working with infectious materials and before leaving the lab 3. Routinely decontaminating work surfaces 4. Work must be performed in a biosafety cabinet
4. Work must be performed in a biosafety cabinet
What BSL category would you anticipate SARS-CoV2 to be considered? Why?
According to CDC guidelines, BSL-2 guidelines should be followed for the majority of lab procedures. Exceptions to this would be during virus isolation, in which BSL-3 guidelines should be followed.
What PPE is required for ebola?
All possible
BSL-2
Associated with human disease- skin injury, in lab coat, gloves, eye protection Biohazard warning signs, sharps precautions PPE: Lab coat, gloves, eye protection are worn *Any procedure that can cause infection from aerosols or splashes are performed within a biosafety cabinet. access to lab is restricted when work is being conducted. Ex: Staphylococcus aureus
What BSL category are the microbes with which we handle in Microbiology Lab? What are the required laboratory procedures to handle these microbes, and what is the required PPE?
BSL-1 and BSL-2 (all BSL-2 work can be performed on the bench because we do not have procedures that could result in splashes or aerosolization). We follow standard microbiological procedures, and we must wear gloves, lab coat, and eye protection.
What are biosafety levels?
Biosafety is the application of safety precautions that reduce a laboratorian's risk of exposure to a potentially infectious microbe and limit contamination of the work environment and, ultimately, the community.
BSL-4
Dangerous/exotic agents, high individual risk of aerosol-transmitted laboratory infection, frequently fatal, no vaccines or treatment. Unknown risk of transmission Agent similar to another used in a BSL-4 lab sealed, negative pressure; All work must be performed within an appropriate Class III BSC (A gas-tight sealed container that is designed to allow for the manipulation of objects), hazardous substances, or infectious disease agents. Class III BSCs have a HEPA filtered air supply and double HEPA filtered exhaust. They provide the ultimate protection for personnel, product, and the environment. -seperate building or isolated zone with dedicated supply of air Shower on exit, clothing change before entry, decontaminate before exiting Full body, air supplied positive pressure suit Ex: Ebola
Why are there 4 biosafety levels? How are they chosen?
Each level has specific controls for containment of microbes and biological agents. The primary risks that determine levels of containment are infectivity, severity of disease, transmissibility, and the nature of the work conducted. (a-SINT. The levels of the biosafety labs aSINT as the varying conditions are changed.) Origin of the microbe, or the agent in question, and the route of exposure are also important
What are the required laboratory procedures to handle SARS-CoV2, and what is the required PPE?
For BSL-2 procedures, standard microbiological procedures are followed, plus access to lab is restricted when work is being conducted. PPE: Lab coat, gloves, eye protection are worn *Any procedure that can cause infection from aerosols or splashes are performed within a biosafety cabinet.
What is the main difference between BSL-2 and BSL-3?
For BSL-3 procedures, BSL-2 guidelines are followed, plus all work occurs in a biosafety cabinet and lab is restricted and controlled at all times.
BSL provides guidelines for handling of a microbe in a laboratory setting. How does this differ in a clinical setting? Are these procedures and PPE more or less stringent?
For healthcare workers interacting with patients with COVID-19, the recommended PPE is an N95 respirator, gown, eye protection, and gloves. This is suitable for a BSL-2 pathogen. However, look at the guidelines to hospitals experiencing a shortage in PPE. Does it seem appropriate to re-use N95 respirators, facemasks, and gowns? What are the potential risks of re-using PPE?
BSL-3
Indigenous or exotic agents that may cause serious or potentially lethal disease Potential respiratory protection Physical separation from access corridors, sel-closing, double-door access, not recirculated air, negative airflow Ex: Mycobacterium tuberculosis
What are the differing biosafety controls
Laboratory practices Safety equipment Facility construction
For ebola, Were the healthcare providers at THR provided with the appropriate PPE? Did they follow the appropriate BSL procedures?
No No
Why are there so few BSL-4 facilities? Are there risks to the community in areas where a BSL-4 facility is located?
Requires high level of expertise from the lab personnel to handle BSL-4 pathogens Extremely expensive to build and maintain a BSL-4 facility Requires expensive biosecurity to maintain safety of facility (security staff, infrared cameras, dual access control locks, cyberlocks to record identity of each person who tries to access facility) Often meet community resistance because of perceived risk to the community if pathogen accidentally escaped/released
BSL-1
Standard microbiological practices are those practices that are common to all laboratories. These practices may include: (1) Not eating, drinking, or applying cosmetics in the lab, (2) washing hands after working with infectious materials and before leaving the lab, (3) routinely decontaminating work surfaces. Work can be performed on an open lab bench. PPE: Lab coat, gloves, eye protection Ex: Nonpathogenic E. coli
