Lab Safety - Questions Microbiology 2420

You may eat a snack in lab as long as there are no bacterial cultures on your bench and you have throughly washed your hands.

False

Long hair should always be tied back in the laboratory

True

Which of the following steps are part of a thorough hand washing protocol? (Check all that apply)

- removal of bracelets, watches, and rings - lathering with soap, rubbing hands together for at least 20 seconds - using a brush and a wooden stick, clean well under finger nails

Beverages are allowed in the laboratory as long as the containers are covered?

False

Infectious laboratory waste is sterilized prior to disposal by

autoclaving (steam autoclave)

When should hands be washed? (Check all that apply)

- Before starting lab activities - After completion of lab activities - Before using the microscope - Immediately after putting on gloves

Which of the following items may be on the laboratory bench top while doing lab work? (Check all that apply)

- Cultures (broth tubes, agar plates, agar slants) - Equipment items specifically needed to complete the lab activity - Pencil or pen

Who and/or what is protected by lab safety protocols? (Check all that apply)

- Students doing lab activities - Lab Instructors and technical assistants. - Anyone who walks into the lab. - The physical lab space and the equipment housed within the lab.

Match each item with its most appropriate means of disposal 1. Used culture plates 2. Paper towels used to dry hands after hand washing 3. Broken glass slides 4. Glass culture tubes containing both cultures of Staphylococcus epidermidis

Biohazard bag - 1 Normal trash receptacle - 2 Sharps container - 3 Rack for autoclaving - 4

Which of the following would be most appropriate for disposal of cultures on agar plates?

Biohazard bag/bin