Lab Tech HW 1 - Blood Sample Collection and Handling
Samples that are not tested within _______ of collection should be _________.
1 hour, refridgerated
When collecting blood with a needle and syringe, what size should they be?
22 G is the general size we use, largest is 18 G and smallest is 25 G
_______________ are required when ___________ or _____________ samples are needed
?
What type of sample should a well-hydrated animal yield?
A well hydrated animal with a 50% PCV should provide 50% plasma and 50% cellular components. A 10 mL sample should yield 5 mL of fluid
When using the Vacutainer system it is important to collect the tubes in what order and why?
Blue White/Plain red Tiger/Red+yellow Green Purple Grey So that the tubes are not contaminated
How is the blood and anticoagulant mixed?
By gentle inversion of the tube 8-10 times
How does EDTA prevent clotting?
EDTA binds permanently to Calcium which is an important mineral for coagulation to occur
How does heparin work?
Forms a temporary bond with antithrombin III which will prevent the formation of fibrin from fibrinogen.
What type of bond does sodium fluoride form?
NaF forms a weak bond with Calcium
Why is using the Vacutainer system ideal when drawing multiple tubes of blood for multiple tests?
Only one needle poke is required for the vacutainer system, it also collects the right amount of blood in each tube. Less pokes + right amount of blood.
Why is the sample better if you properly use the Vacutainer system?
The sample is better because the Vacutainer system only pulls blood through the needle one time and goes straight into the tube - which collects the right amount of blood. This means that there is less of a chance that the blood cells have been damaged by going through the needle several times and through the tops of tubes.
What does the word hematology mean?
The science involved with the study of blood cells and their formation
What is an anticoagulant?
a substance that inhibits blood clotting
Refrigerated samples must be _________ and _________ before analysis.
brought back to room temperature and remixed
EDTA should not be used for plasma samples needed for _________.
chemistries
Heparin anticoagulant should never be used for ________________ because the anticoagulant __________________.
differential blood film analysis, interferes with the staining of WBC
Sodium fluoride is used mostly for _____.
glucose testing
The production of blood cells and platelets is called _______.
hematopoiesis
__________ is a suitable anticoagulant for most samples that require ___________.
heparin, plasma
Why is EDTA the preferred sample for hematologic studies?
it does not alter cell morphology when used in the right proportion ratio for samples to be analyzed soon after collection
Name the agranulocytes.
lymphocytes and monocytes
List the granulocytes.
neutrophils, eosinophils, basophils
What does a Complete Blood Count (CBC) include?
red and white blood cell counts, hemoglobin concentration, packed cell volume, a differential white blood film, examination, and calculation of absolute values and erythrocyte indices
The cellular component of blood is made up of ______.
red blood cells, white blood cells, thrombocytes
Shaking a tube of blood causes what?
shaking will cause damage to blood cells such as hemolysis
What two salts is EDTA available in?
sodium and potassium
After swabbing the blood collection site with alcohol, what is the next step?
the alcohol must be allowed to dry before proceeding to next step
Plasma is_______.
the liquid portion of the blood
What is the preferred method of collection?
through a Vacutainer system
Why is it important to gently mix anticoagulant blood tubes before running tests?
to make sure all the blood in the sample has evenly been mixed with the anticoagulant and that no clots are present
What is almost always the preferred source of blood, and where is the preferential site for collection?
venous blood from the jugular vein
Hematology testing primarily involves the use of _________ _________ sample.
whole venous blood
How do citrate anticoagulants work?
Citrate anticoagulants form a permanent bond with Calcium but can be reversed
