manduca quizzes

what the specific gravity of the floatation media used inlad

1.25-1.30 SG

What is the calculated estimate of the Hb value for a cat with a PCV of 30%

10 g/dl

Whole blood can be stored in the refrigerator for up to___without changes in quantitative RBC parameters

24 hours

the magnification of the high dry objective is

40x

what is neutrophil hypersegmentation

5 or more lobes in the nucleus of the neutrophil

Which is consistently seen with hemangiosarcoma (HSA) of the liver in dogs

Acanthocytes & schistocytes

What does Vetscan Rapid Canine Heartworm Test detect?

Antigen of female canine heartworm

EDTA can cause platelets to clump,especially in horses. The use of a____top tube may reduce PLT clumping

Blue

What breed of dog often has giant platelets and/or low platelet counts

Cavelier king charles spaniel

what is the different between cat and dog heartworm

Dog can be tested more for antigen positive then cats (mostly test for anitbody to diagnose) The cat lungs are more affected than dogs (heart and sometimes they lungs) Cat heartworm are occult and it will have one sex adut heartworm that won't produce microfiliaria

Serum/plasma that is yellow called

Icterus

What is the signifiancance identifying spherocytes on a blood film

Immune mediated hemolytic

polychromasia is identifed on a peripheral blood smear by the presence of

Large basophilic RBC

which is erythrocyte parameter gives an indication of the size of a eythrocyte

MCV

Explain why a significant number of microcytes in circulation will result in a decreased MCV

MCV measure the volume of rbc so smaller (microcytic) rbc will cause a decrease in volume

If the automated platelet count is below the normal range, what should be done before accepting the machine low count as accurate

Make a blood smear to determine if there macroplatelet or platelet clumping and do a platelet estimates

List 2 thing that are important to know when performing SNAP tests

Make sure everything is prepare & at room temperature, most be done on a flat surface

List 3 changes in RBC seen on the blood film that may indicate regenerative anemia.

Metarubricytosis,polychromasia , increase # of Howell-Jolly bodies and basophilic stippling

red top tubes contain edta? heparin? citrate? None of the above

None of the above; serum

what is the most common cause of a fasley elevated pcv due to inadequate packing of rbc

Not spinning the blood sample long enough (5 minutes) in the microhematocrit

the term for an increase in the number of abnormally shaped RBC is

Poikilocytosis

Name a dog breed that may have a decreased MCV WITHOUT being anemic

Shiba Inu, Chow Chow (chinese breed)

a neutrophilic band is

a immature neutrophil

which type of feline reticulocyte is a polychromatophil on romanowsky stained blood film?

aggregate

what causes or enhances hypersegmentation of neutrophils

all of the above (cell aging, treatment with steriods, hyperadrencortisolism)

Activated Platelets

all of the above (may have a spider live appearance MAy form large clumps or masses of PLTS May have vacuoles, decreased granularity or less intense staining

what is the term for decrease rbc mass

anemia

Decreased erythrocyte mass is called

anemic

why must green top tubes be used to collect blood for reptile cbc?

because edta causes erythrocyte lysis in most reptiles

why can't we use the fecal floatation procedures to find the trophozoite form of Giardia

because the solution will kill it

why is lens cleaner used in sparingly?

chemicals in lens cleanser mess up adhension in objective lenses

Feline platelets commonly are

clumped and as large as RBC or larger

what the definition of lymphopenia

decrease number of lymphocyte

increases in both pcv and tp may be interpreted as

dehydration

what is hemolysis

destruction of rbc

What fecal examination method is used to identify motile forms of protozoa

direct smear

purple lavender top tubes contai

edta

true or false,immersion oil should always be used with the 40x objective

false

true or false: Sodium nitrate flotation solution is the least desirable floatation solution because it corrodes expensive laboratory equipment and distorts parasite ova morphology

false

true or false: heparin imparts the best preservation of cellular morphologic detail

false

true or false: sugar floatation solution is the least desirable floatation solution because it corrodes expensive laboratory equiment and distorts parasite ova morphology

false

true or false: when using the microscope to evaluate a blood smear using oil immersion, less light is needed that when looking at fecal sample

false; more light for oil immersion

true or false: aggregation of rbc into linear formation resembling stacked coiins is called aggluantion

false; roulex

true or false: more light is needed when evaluating is direct fecal smear than when using oil immersion

false;less light for direct smear

true or false: opaque, "milky" plasma/serum is called icteric

false;lipemia

true or false: Mammalian platelets are round to slighty oval or slight elongated and have a small central purple nucleus and fine cytoplasmic granules

fasle;avian

what cause lipemia/ this changes in

fat in the blood

the ova of which of the following parasite will not float

flukes

The storage of whole blood at temperature above temperature can cause__

hemolysis, falsely elevated RBC counts, falsely elevated hemoglobin (Hb) values

green top tubes contain

heparin

list 4 different parameters that can be evakuated by spinning whole blood in a microhematocrit tube

hydration status, protein concentration & pcv, tp,estimate of wbc

what does the term leukocytosis mean?

increase in normal values of wbc

what is monocytosis

increase number of circulating monocytes

WHAT DOES THE TERM LEUKOCYTOSIS MEAN

increase wbc

the defintion of basophilia

increased number of basophils

what does the term eosinophilia means?

increased number of eosinophils

what is the term for a high number of neutrophilic band in the peripheral blood

left shift

what is the term for an increased number of neutrophilic bands

left shift

what morphologic characteristics are used to differentiate Dirofilaria immitis microfilariae from those of other filarid nematode?

length, width,head shape

the microscope objective lenses should be cleaned with

lens paper

serum/plasma that is opaque, "milky" plasma/serum is called

lipemia

The presence of increased numbers of acanthocytes often is indicative of

liver diease

List one general cause of yellow plasma/serum

liver disease

what is the best index of decreased rbc mass

low pcv valve

What does an increase MCV in an anemic animal suggest?

macrocytic

when we measure the packed red cell column on a spun microhematocrit what are we assessing in the animal?

mass of rbc in circulating blood

List 2 test detecting microfilariae in canine blood

modified knott test, filter test, direct smear,blood film, microhematocrit tube

young platelet are

often large as RBC or larger fully functional called megaplatelet,macroplatelet, shift platelet

reticulocyte count are performed using which objective power

oil immersion(100x)

what cause neutrophil hypersegmentation

old age

How is anemia noted clinically

pale gums/ pale mucous membranes

which of the choices below is the best index of rbc mass

pcv

hb formula

pcv/3

what the different between plasma and serum?

plasma contain fibrinogen serum does not

List 2 reason why the hematology machine might give low PLT result

platelet clumping and the presence of macroplatelet

Name a breed of that dog that may increase MCV without being anemic

poodle and greyhound

when a drop of plasma or serum is placed on the refractometer prism, most often it is ___ of the sample that is being evaluated

protein concentration

where in the body adult canine heartworm live

pulmonary arteries on the ride side of the heart

Which reticulocyte is most prevelant normally in domestic cats

punctate

What type of blood tube is used to produce serum

red top tube or tiger top tube

list variables, stated in the article on fecal examination, which can directly affect the result of a fecal examination

sample size(2g centrifuged); 1g standing, timing,,timing, make sure the sample is fresh

Small, irregularly shaped RBC fragment caused by mechanical trauma while in circulation within the vascultature

schistocyte

the fecal flotation media used in our lab and in most small animal pratice is

sodium nitrate

what cause monocytosis

stress

2 general cause of eosinophilia

stress and hypersensitivity/allergic

the condenser and diaphragm are used to

take full advantage of the microscope resolving capacity and adjust the amount of light

how can one tell that hemolysis has occured?

the plasma appear pink/red

Which of the following does not describe a spherocyte?

they can be identified in most domestic species

What can directly affect the result of a fecal examination

timing and history of the patients if the fecal sample not fresh If you don't use the correct sample size

What is the purpose of the microscope diaphragm aperture>

to control the amount of light

why should blood films be prepared immediately after blood collection?

to reduce artifacts in cell morphology caused by anticoagulants

true or false: Sodium chloride flotation solution is the least desirable floatation solution because it corrodes expensive laboratory equiment and distorts parasite ova morphology

true

true or false: edta imparts the best preservation of cellular morphologic detail

true

refractile artifact in rbc on a blood film stained with diff-quik stain is usually due to

water/moisture in the fixative

what does the buffy coat contain

wbc and platelet

list the type of cells found in whole blood

wbc, rbc, platelets

give an example of when ppe always must be used in the veterinary labatory

when handling human/primate speciemens, speciemens, and any suspected of having zoonitic and pathogenic

Which flotation solution is the best one to use for floating Giardia cysts?

zinc sulfate