Mycology

Endothrix

the presence of spores on the interior of the hair shaft

What is 10% KOH used for?

Direct exam of dermotaphytes

Mycosis

Disease caused by fungal imfection

DTM should be incubated for how long?

up to 30 days

Trichophyton mentagrophytes

zoophilic (animal loving)

Microsporum gypseum

zoophilic (animal loving) and geophilic (environment loving)

Blastomyces dermatitidis

Dogs and humans Clincally produces granular, weeping ulcers and abscesses in the lungs. Systematically spreads to other organs, tissues, and skin

ELIS Test

Enzyme-linked immuno assay. Commonly used to defect antibodies in the blood.

Fermentation

Enzymes which cause chemical changes to sugar under anaerobic conditions

What can cause a false positive when using a Wood's Lamp?

Epidermal scales and some soap residues

Dermatophyte Test Medium (DTM)

Is a selective and differential medium which turns red ( phenol red indicator) as a dermatophtye grows and produces alkaline metabolic products. Inhabits bacterial growth

Sabouraud Dextrose Agar

Is the standard medium for fungal isolation. This medium is selective for fungi and has no color. Has antibiotic in the medium.

Mycelium

Is the vegetative part of the fungus, consisting of branches , thread-like hyphae

When preparing a Culture Tape Prep for dermatophyte, what type of stain is used?

Lactophenol Cotton Blue Stain

Fungus

Microscopic plants that do not contain chlorophyll for photosynthesis and grow in irregular masses without roots, stem or leaves.

What genius and species dermatophyte does the Wood's Lamp fluoresce?

Microsporum canis

Yeasts basic structure/appearance

Peanut or bowling pin shape

Tina

Ringworm

Serology

is the scientific study of plasma serum and other bodily fluids. In practice, the term usually refers to the diagnostic identification of antibodies in the serum.

Candida albicans

"Thrush" found normally in mucous membranes Oval budding yeast that produces a thick-walled chlamdospore when grown in corn meal agar

Dermatophyte

A fungus that causes infection in the skin, hair and/or nails

What color does Microsporum canis fluoresce?

Brilliant yellow-green color

Penicillin

Derived from dust and soil molds

Malassezia pachydermatis

Oval, bottle-shape, or monopolar budding yeast. Dermatitis

After the ear swab collection, what is the correct transfer method on to the slide?

Rolling swab back farther across the slide vertically.

Hyphae

Strands resemble branches and roots

Mycology

The study of fungi that produce disease and the study of the diseases they produce

Aspergillus fumigatus

Usually saprophytic, associated w/ poutry Causes progressive paranasal inflection, lung infection, and toxicosis

Arthrospores

arise from the breakdown of hyphae

In what conditions should a DTM be incubated?

away far UV light at 22-25 degrees Celsius

Macro-conidia

borne on the hyphae or on short pedicels. Thin or thick walled

Saprophytic

breaks down and lives off dead organic matter

What is the light wave length on the Wood's Lamp?

roughly 3650 Angstroms

Ectothrix

the presence of spores on the exterior of the hair shaft

DTM Phenol red indicated takes how long to change the yellow agar to red?

within 14 days

Microsporum canis

zoophilic (animal loving)

Micro-conidia

Are club-like shapes to pyroform and form along the sides of the hyphae Thin walled

Rods

Bacterial Gram Negative

Cocci

Bacterial Gram Positive. As a spherical, Ovoid, or general round shape.

Wood's Lamp has what type of light?

Black light

Aleuriospores

Are a type of fungal spore typically produced by segmentation of pre-existing fungal hyphae.

Cryptococcus neoformans

Cats, dogs and sometimes horses Causes progressive paranasal inflection frequently leading to CNS infection

Mycelia

Cilia or hairs (masses of hyphae)

Trichophyton mentagrophytes (DTM)

Colonies are flat with cream to white cotton powdery surface that grows in concentric rings and rays. The reverse pigment is dark red or yellow to rose.

Microsporum canis (DTM)

Colonies are white and coarse fluffy. Reverse pigment deep yellow orange pigment.

Microsporum gypseum (DTM)

Colonies spread rapidly and have a coarse powdery surface with a cinnamon-brown center. Reverse pigment is usually dull yellow or tan to yellow-cream

When doing an Ear Cytology what type of stain is used?

Diff-Quik or Gram stain