Practical 2
how many NADH molecules are generated in the citric acid cycle from each glucose that enters
6
which of the following wavelength settings is best for having a spec 20 detect the absorbance of light by a substance that is blue in solution
600
when oxidized DPIP is mixed with illuminated chloroplasts what happens
DPIP gains electrons and loses its blue color
in the oxygen production lab, what compensation was made for the heat generated by the lamps
a tank of water was placed in the light path to absorb heat
why is there a leading strand and a lagging strand during DNA replication
because polynucleotide strands are antiparallel
which of the following is/are true of the solubilities of the pigments studied in lab
carotene is more soluble in ethanol than in petroleum ether
In the oxidation of water lab that utilized the blue dye DPIP which of the following represents a measured(dependent variable)
change in absorbance over time
which of the following is/are true of the absorption spectrum of chlorophyll b as studied in lab
chlorophyll b had an absorption peak at 640 nm and chlorophyll b has an absorption peak at about 460 nm
which of the following exercises that you performed in lab best shows that a chloroplasts play a crucial role in the color change of DPIP
comparing the absorbance changes in seperate tubes in which increasing amounts of chloroplasts were added
which of the following pump H+ ions as part of the e- transport chain
complex I complex iii
the main goal of the chlorophyll extraction lab is to
determine the absorption spectrum of pigments used in photosynthesis
a sequence of nucleotides that codes for a specific molecule is a
gene
which of the following is true about DNA polyermase
it can only attach new nucleotide to 3' end of an existing nulceotide
what purpose does the spec 20 blank serve
it ensures that the measured absorbance is due only to the pigments
which of the following is done to determine the absorption spectrum of a substance
measure its absorbance over a range of different wavelengths of light
which of the following is done to determine the absorption spectrum of a substrate
measure its absorbance over a range of different wavelengths of light
which respiration pathway generates the most ATP
oxidative phosphorylation and e- transport chain
what do chloroplasts and sodium bisulfite hold in common
oxidize DPIP, reduce DPIP
which of the following do chloroplasts and sodium bisulfite hold in common
sodium bisulfite and chloroplasts may cause DPIP that is initially blue in solution to become colorless
which of the following are capable of reducing DPIPox
sodium bisulfite and illuminated chloroplasts
in the oxygen production lab, how was PFR varied
the PFR was varied by adjusting the distance between the light source and the tube containing chlorella
a buffer was used in the dye reduction lab. What purpose did the buffer serve?
the buffer helped resist changes in pH in the chloroplast preparation
which of the following is true of the spectrophotometer calibrations in the chlorophyll extraction lab
the callibrations must be repeated for each change in solvent the calibrations must be repeated for each wavelength used to construct the absorption spectrum
the absorption spectrum of carotenes as determined in lab sometimes showed an absorption peak in the red region of the spectrum. However the absorption spectrum of carotenes shown in the textbook did not show such a peak. What caused this difference
the carotenes extracted in our lab were contaminated with chlorophyll; the carotenes used in the textbook were not contaminated with chlorophyll
why is harvesting energy from electrons in several steps more efficient than doing it all at once
the cells gets more of the available energy
the reduction of DPIP by chlorplasts was coupled to which reaction below
the oxidation of water
note the electron carries in the thylakoid membrane
the poison blocks the transfer of electrons from 1 to 2
which of the following best represents the dependent variable in the dye reduction lab
the rate of water oxidation
which of the following best represents the independent variable in the dye reduction lab
the volume of the chloroplasts added to the experimental tube
what was the purpose of using a buffer for the isolation and function assessment of chloroplasts
to eliminate pH as a variable in the experiment
which of the following best explains the purpose of using a buffer in the dye reduction lab
to prevent changes in pH in the chloroplast preparation
in the reaction you studied in the dye reduction laboratory, where did the electrons that changed the DPIP come from
water
