Pre lab quizzes
100μL is equivalent to:
0.1mL
What voltage is gel electrophoresis run at?
100V
Ocular lenses found in most compound light microscopes a magnification of..?
10x
What is the optimal temperature for most enzymes?
20-40 degrees Celsius
How long is gel electrophoresis typically run for?
30 minutes
You are using a compound microscope and you have the objective lens set to 40x. What is the total magnification you are using to view the specimen?
400x
What is used to compare observed ratios for a given cross compare with predicted ratios?
Chi-square analysis (χ2)
What color-producing chemical will be used in today's lab to determine the concentration of glucose solutions through absorbance readings?
Chromogen
__________ is more resistant than ___________ to processing and can be extracted from highly processed foods, making it a better option for identifying GM in food.
DNA and proteins
What happens if either the enzyme or substrate is limited?
Enzyme reaction will remain constant
If the results of a patient's two "random" glucose readings are 196 mg/dL and 200mg/dL, this person meets the criteria of diabetes mellitus.
False
What will happen when a red blood cell is placed in distilled water?
Lysis will occur
What determines resolution?
Magnification, Lens Quality, Lens diameter and visual acuity
Can dominant alleles be masked by recessive alleles?
No
When leaf pigments absorb light, electrons are boosted to a higher energy level, providing energy to:
Reduce NADP+, and produce ATP
Gel electrophoresis separates DNA on the basis of what?
Size of DNA
What is added to a gel that allows us to visualize DNA?
SyberSafe dye
What type of buffer is used to run gel electrophoresis?
TAE
What will happen in the experiment when light hits the chloroplasts?
The DPIP is reduced, changes from blue to colorless, and there will be an increases in light transmittance
Independent variables are usually plotted on the X-axis of a graph, whereas dependent variables are usually plotted on the Y-axis
True
Potential allergic reactions to novel proteins and Antibiotic resistance from selectable markers are major arguments against/concerns associated with the GMO industry
True
When an enzyme's reaction rate doubles for every 10 degree Celsius increase in temperature it's called the Q10 effect.
True
If you were given a sample of bacteria to analyze, which microscope would be best for quickly identifying the specimen?
compound
During the process of gel electrophoresis, combs are used to
form the wells that samples will be placed in
At which stages of aerobic cellular respiration are ATP produced?
glycolysis, krebs cycle, chemiosmosis
There is a(n) __________ relationship between the color of a solution and the wavelength of light it best absorbs.
inverse
What is the in vitro substrate for alkaline phosphatase (ALP) that we will be using in lab?
pNPP
DNA from Bacillus thuringiensis is incorporated into the genomes of crops to make them
pest resistant
ALP catalyzes the hydrolysis of _______________ attached to proteins or lipids.
phosphate groups
Large complexes of proteins and pigments (light-absorbing molecules) that are optimized to harvest light are called:
photosystems
Which of the following molecules can diffuse through a cell membrane?
small molecules
What factors determine how fast water moves across a membrane?
solute concentration difference & hydrostatic pressure
What color will the product of today's experiment (pNP) turn?
yellow
