Review

Specific Criteria for Euthanasia (4)

-- -- -- --

Which tank systems uses the greatest amount of water?

Flow- through

Type of aquatic life support system has a biological filter

Recirculation system

Acceptable with conditions

Techniques that may require certain criteria to be met to consistently produce humane death.

True or False: All animals should be housed in between 68F and 79F dry bulb temperatures.

False

True or False: All species eat what is good for them in appropriate amounts, so scheduled feeding is not necessary for any species.

False

True or False: Floor drains are necessary in ALL animal rooms.

False

True or False: Environmental factors that may affect breeding programs include light cycle, temperature, humidity, and noise.

True

List decontamination procedure greatest to least effect.

Sterilization, disinfection, cleaning

Concept of Reduction

Strategies that result in the same scientific outcomes with using fewer animals per experiment

True or False: Restraint can negatively contribute to an animal's health.

True

Acceptable

techniques that consistently produce a humane death when used as a sole means of euthanasia.

True or False: If a department within an institution maintains its own animal facility and operating budget, it is considered a centralized management system.

False

True or False: It is acceptable to give euthanasia within the muscle (IM) or under the skin (SQ).

False

Individual Ventilation Cages control...

Air quality

Name the type of floor plan with a single clean corridor that serves the entire facility and is the most expensive due to the duplication of corridors.

Three Corridor floor plan

Fixed Cost

consistent throughout the budget period

Autoclave

equipment sterilizes surgical equipment, housing, feed, water, and bedding.

Factors predisposing animals to disease

genetic, environmental, metabolic, experimental, dietary variables

An occupational health and safety program is designed to protect

Animal Care Personnel

Indirect Cost

incurred by animal facility not specified to identification of particular animal species or project

A room that require bioexclusion needs what type of ventilation?

Positive pressure ventilation

Which of the following is not important information to have on a feed storage container?

The animal protocol number

True or False: The Guide for the Care and Use of Laboratory Animals is an essential source of information on animal care and use program management and is considered the "gold standard" guidelines for laboratory animal facilities.

True

True or False: The IACUC must notify the Office of Laboratory Animal Welfare if any serious deviation from the Guide endangers animal care and welfare.

True

True or False: The humane end point is the earliest scientifically justified point at which an experimental animal's pain and/or distress can be prevented, terminated, or relieved.

True

Concept of Replacement

Using invertebrate animals instead of vertebrate animals in research Using non animals methods of obtaining scientific data

NOT a recommendation for how to store feed

In a room with high temperature

Best storage conditions for non opened bags of animal bedding

In a separate room away from the animal rooms, stacked on pallets, 6 inches away from the wall.

Concept of Refinement

strategies resulting in procedures that enhance animal well-being and minimize pain and distress.

What type of equipment has HEPA filtered air into and out and is used for high-risk biological agents?

Class III biological safety cabinet

Four different ways a lab technician can be exposed to infectious agents during animal procedures.

Airborne Needle to Stick -- --

Animal room doors within the facility

Allow easy passage of racks and equipment Self-closing Construction of materials resistant to corrosion and moisture View-windows for safety

Unacceptable

Methods deemed inhumane under any conditions, may have a substantial risk to the person applying the technique.

Animal Welfare Act

Minimum of 3 members on the IACUC Conducts inspections annually

Which of the following is false about security breaches and threats of violence?

Most threats are not serious so there is no need to take them seriously

Animal quarantine rooms and rooms containing animals experimentally infected with pathogens are normally kept under what type of pressure?

Negative

In a biocontainment facility, primary biocontainment barriers are designed to do what?

Negative pressure ventilation contains agents in the facility or area

Shipment of 100g rats to your facility. Dimensions of cage: 8in (L) x 10in (W) x 5in (H) How many rats can you place in each cage?

None of the above.

Difference between subjective and objective factors that may influence the proper management of laboratory animal facilities. Use specific examples.

Objective -- Subjective --

Not a potential environmental cause of disease

Odor

______ rates refer to the cost charged to maintain one unit, typically either one animal cage or cage of animals, for one day.

Per diem

Good Laboratory Practices (GLP) are most likely to be mandated for what type of study?

Pharmaceutical studies

Variable Cost

based upon demand and include most expendable supplies and labor costs for husbandry and technical staff.

Describes feeding by free choice

Ad libitum

Recommended number of air changes in an animal room

10-15x

Average daily census: 220 hamsters Cost of feed, bedding, caging, salaries/ year: 100,000 What would their per diem rate be per hamster?

$1.25

Conversions: 1g = ? mg

1000mg

Which of the following needle gauges is the biggest?

16G

Which suture size is the largest in diameter? 5-0, 3-0, 6-0, 2-0

2-0

Sodium Thiopental has a concentration of 2.5%, convert this to mg/ml.

25mg/ml

Duration of protocol once IACUC approves

3 years

Light intensity is set by the Guide. It states that light intensity of ______ lumens is adequate for routine animal care activities.

323 lumens

Corridors should have a minimum clear width of __________

6ft

You are the head laboratory animal technician in a major research lab at the CDC. You just received 4 shipments of mice (20 in total). The average weight of the mouse is 25g. The cage dimension is 8in in width, 11.5in in length, and 7in in height. Using the chart below calculate how many mice can be housed in one cage.

7 mice

NOT an emergency situation within a laboratory animal facility

A nonhuman primate that has destroyed its cage card

Ventilation

Adequate oxygen supply. Removes thermal loads caused by animals, personnel, lights, and equipment. Dilutes gaseous and particulate containments, like allergens and airborne pathogens. Adjusts moisture content and temperature of room air. Creates air pressure differentials between adjoining spaces.

Who is ultimately responsible for all animal care within an institution (academic or private)?

Attending Veterinarian

Five members required on IACUC committee

Attending Veterinarian Non-affiliated, practicing scientist (research with animals), nonscientific, faculty member of University (research experience)

This is used for treating material to be sterilized with pressurized, saturated steam for 15 min at no less than 250*F

Autoclave

IACUC protocol considers

Availability of alternative procedures or models Justification for the number of animals requested Qualifications of personnel

Public Health Service Policy

Enforced by OLAW Inspections are only for cause Covers all vertebrates

How often should you check on your sentinels?

Everyday

The two general types of adverse effects are

Extensive Contained

In colony management the most common error is

Failure to notice

True or False: Surgical areas are more efficient when incorporated into a decentralized facility.

False

True or False: The Guide is a regulatory body that all research facilities must comply with.

False

True or False: The goal of imaging is to used as much radiation as possible.

False

True or False: Ventilation of the microenvironment ensures adequate ventilation of the microenvironment.

False

species requires vitamin C as a portion of their diet

Guinea pig Primates Homo sapiens

Oldest and most familiar agent in killing microorganisms

Heat

Cages allows for active movement of air into and out of the cage

Individually ventilated caging

IACUC

Institutional Animal Care and Use Committee

Who is ultimately responsible for ensuring federal regulation compliance regarding the use and care of laboratory animals within an institution?

Institutional Official

Barrier Facilites

Items leaving the facility must be autoclaved.

Signs of disease in Aquatic (Fish)

Lethargy, Darting, Fin Erosion, Piping, Change in pigmentation

possible hazard in lab animal facilities

Protocol-related hazards, including chemicals of unknown hazards and infectious agents. Physical hazards, including sharps, flammable materials, lighting, noise, radiation, and pressure vessels. Zoonoses

The word _____ is used in the Guide to describe aspects of a program that are strong recommendations to achieve a goal.

Should

Name the type of floor plan with no separation of traffic by clean and dirty status.

Single Corridor floor plan

Which aquatic microenvironment tank system would require frequent water changes?

Static

BSL-4

Work with dangerous and exotic agents that pose a high individual risk of aerosol-transmitted laboratory infection and life-threatening disease.

BSL-3

agents may cause serious or lethal disease by the inhalation route.

Decentralization of a laboratory animal facility pertaining to the functional areas and specialty laboratories is described as

an animal facility where animal house and use occur in a space not solely dedicated to animal care and support.

BSL-2

associated with human disease, which rarely serious and for which preventative or therapeutic interventions are available.

Direct Cost

attributions specified to a research project (EX; cost of animals)

Rat grimace scale includes all of the following observations except

cheek bulge

ASBL-3 and higher

contaminated bedding, cages, carcasses, and equipment should be decontaminated before removal from animal housing in animal biosafety levels.

BSL-1

little or no known potential hazard to animal handling personnel and the environment

Minimum number of air changes in an animal room

once

By mouth

per os (PO)

Lunch/Breakroom should be positioned within a laboratory animal facility where

rooms should be used for employees to eat and rest, they should have a separate airflow from the rest of the facility.