The Ames Test
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List: Steps in the ames test
1. strain of histidine-requiring salmonella typhimurium is inoculated into medium lacking histidine 2. Suspected chemical mutagen is added to a well in the medium to diffuse throughout the medium 3. If the salmonella grows, a mutation has occurred which allows salmonella to now make its own histidine meaning that the chemical is a mutagen
Answer: What is the medical significance of the ames test
90% of the agents that can cause cancer in humans can also cause mutations in bacteria. so a mutagen for bacteria might be a potential human cancer agent
Define: The ames test
test to determine if a chemical can induce a mutation in a bacteria
