The Ubiquitous Microbes
You can not sterilize your hands
100% like you would wished for
Then, rub the swab on your hands and inoculate the plate labeled
37° by gently rubbing the swab over the surface of the agar on half of the plate.
microbes that grow in warm blooded animals prefer temperatures close to
37⁰C, and may not grow well at lower or higher temperatures
What routine methods are used to remove microbes from surfaces ? Are they effective on the surfaces you sampled ?
A good method to remove microbes is to wash hands with soap and for 20 seconds It is effective but not the best or worse method
Agar is found in
Algae
Why were the samples from the hand washing exercise incubated at 37c?
Because we used our hands for handwashing and our body temperature is 37^.c
For the organisms that grew on agar, what did they use for nutrients and water in the environment?
In the environment , the moisture and for nutrients our body like dead skin cells
We use glass or
Paper plates
Agar is used for
Solidifying the food source / culture media because it does not affect the growth of bacteria
a bacterium that grows well in your throat might find
TSA lacking in nutrients they require.
Why were the environmental samples incubated at room temperature? Would the same organisms grow in your body ? Why or why not ?
They were sampled in room temperate because we collected the bacteria in room temperature No the organisms can not grow the same because our bodies would be too hot for the ones grown in room temperature
Organisms cannot be seen with the naked eye are?
bacteria, archaea, protozoa, algae, fungi, viruses, viroids, and prions.
Place the plate upside-down in the 37° incubator. Plates are always placed upside-down in the incubator
because condensation accumulates on the lids and will drip on the cultures otherwise.
A common characteristic of microbes is that most
cannot be seen with the naked eye.
Microbes are present in most environments on Earth and are responsible for causing
disease and for carrying out many of the essential reactions in nutrient cycling, from the generation of O2 and organic nitrogen to the decomposition of biological remains.
The study of microbiology generally focuses on the
interaction between microbes and the immune system of humans in both negative and positive ways.
Microorganisms (microbes) are ubiquitous,
meaning they are found everywhere - in the air, soil, water, on plants, in mountains; even in extreme environments such as hot springs, and Antarctic ice
The other consideration is atmosphere; many bacteria require
oxygen, whereas oxygen is toxic to other bacteria. Therefore, the organisms you see growing on your TSA plates may represent only a fraction of the organisms formerly present.
Inoculate the plate labeled 25° by gently rubbing the swab over the surface of the agar on a quadrant of the plate when testing
room temperatures
When attempting to grow microbes one must consider
the type of media, the temperature, and the atmosphere which will allow optimal growth
The sampled material testing will be placed on plates containing
trypticase soy agar (TSA) a nutrient rich media that supports the growth of many diverse organisms.
Moisten a sterile cotton swab
with sterile water.
