what is the function of

Phenol

help in removing protein impurities from the DNA to get pure DNA

iso propanol / ethanol

to precipitate the DNA by engaging with the water molecules as not giving chance for the DNA to get dissolve in the water

Beta-mercaptoethanol

strong reducing agent which can remove tannins and other poly phenol often present in the crude plant extract

PVP

1)assist to remove contaminants 2)remove phenolic compounds from the plant DNA extracts 3)form H bonds with the phenolic compounds

Isoamyl alchol

1)its anti foaming agent that reduce foaming during isolation 2)it stabilizes the interphase between aq and organic phase which is protein

CTAB

1)use as a detergent for cell lysis 2)assist in the removal of contaminants 3)getting rid of polysaccharides

SDS

1)use as a detergent to lyse the cell 2)remove lipids ans proteins molecules 3)decrease surface tension to be easily to get rid of ribosomes 4)getting rid of di sulphide bond in quartenary or complex protein to make tertiary then secondry then primary protein which is the simplest form

EDTA

in activation of DNases ,RNases also used as a co_factor

DEPC

inactive nucleases enzymes by covalenty

Chloroform

prevent shearing of DNA during isolation

NaCl

provide Na ions that will block negative charge from phosphate on DNA negatively charged phosphates on DNA cause molecules to repel each other ,Na ion will form an ionic bond with the negatively charged phosphate on the DNA and also neutralizing the negative charges and allowing the DNA molecule to come together