Assays

Alkaline phosphatase

Antibody-coupled enzyme; p-nitrophenyl-phosphate (colorless) to p-nitrophenol (bright yellow) + phosphate

Bradford

Comassie Brilliant BLUE dye binds to protein.

BCA

Copper ion based protein assays. the protein solution is mixed with an alkaline solution of copper salt. Cuprous ions detected with BCA. PURPLE

Lowry Method

Copper ion based protein assays. the protein solution is mixed with an alkaline solution of copper salt. Cuprous ions detected with Folin Reagent

ELISA

Standard Enzyme-linked immunosorbant assay; case: HIV coat proteins in human serum.

PI-SceI endonuclease

colorimetric assay; examine UV absorbance

B-galactosidase

colorimetric assay; hydrolyzes B-galactosides into monosaccharides. Galactose--> Blue color

Ability of biuret to chelate Cu2+ suggested that...

peptide bonds could chelate too

Thymidylate synthase

radiochemical assay; [5-3H] dUMP to dTMP + dihydrofolate. filter through activated charcoal

Hexokinase

radiochemical assay; phosphorylates a 6-C sugar; disk of DEAE cellulose filter paper

Lactate dehydrogenase

spectrophotometric assay; pyruvate to L-Lactate; UB absorbance