Assays
Alkaline phosphatase
Antibody-coupled enzyme; p-nitrophenyl-phosphate (colorless) to p-nitrophenol (bright yellow) + phosphate
Bradford
Comassie Brilliant BLUE dye binds to protein.
BCA
Copper ion based protein assays. the protein solution is mixed with an alkaline solution of copper salt. Cuprous ions detected with BCA. PURPLE
Lowry Method
Copper ion based protein assays. the protein solution is mixed with an alkaline solution of copper salt. Cuprous ions detected with Folin Reagent
ELISA
Standard Enzyme-linked immunosorbant assay; case: HIV coat proteins in human serum.
PI-SceI endonuclease
colorimetric assay; examine UV absorbance
B-galactosidase
colorimetric assay; hydrolyzes B-galactosides into monosaccharides. Galactose--> Blue color
Ability of biuret to chelate Cu2+ suggested that...
peptide bonds could chelate too
Thymidylate synthase
radiochemical assay; [5-3H] dUMP to dTMP + dihydrofolate. filter through activated charcoal
Hexokinase
radiochemical assay; phosphorylates a 6-C sugar; disk of DEAE cellulose filter paper
Lactate dehydrogenase
spectrophotometric assay; pyruvate to L-Lactate; UB absorbance