bio 1001: lab practical 2
plasma membrane
(cell lab) the dialysis tubing in the experimental procedure stimulates a....
starch did not diffuse bc the molecules are too large to diffuse from the tubing
(cell lab) which solute did not diffuse across the dialysis membrane from the bag to the beaker? how do you know?
more bubbles
According to test results what happened when you increase substrates
Gluclose
Based on results of Benedict's test, what solute diffused across the dialysis membrane from the bag to the beaker?
water and oxygen
Catalayse takes H2O2 and turns it into....
It maintains the lens parameters and strength of the solution
Contact lens solution is described as sterile, buffered, isotonic aqueos solution. Explain the importance of the adjectives buffered and isotonic to the person buying the solution
osmosis
Diffusion of water through a selectively permeable membrane
diffusion
Movement of molecules from an area of higher concentration to an area of lower concentration.
halose
New enzyme breaks down tree halase
There is an an abundance of Smooth ER
Ovarian follicle cells produce estrogen, which is a steroid. What organelle will be present in abundance so that the follicle cells might perform this funciton?
Degradation Enzymatic Reaction
Since enzymes are specific for certain substrates and often have similar names, what is the name of the enzyme used in tube 3 of the catalase activity experiment that facilitates the breakdown of sucrose?
Activation energy
The enzymes lowers this energy. what does it lower- ___________________ (barrier)
Nucleoid
What are the organelles that are specific to a bacterial cell?
affects hydrogen bonding and structure of the enzyme
What do changes in pH (too high or too low) do to an enzyme that reduces its activity
warms up should have increased activity
What happened when you put it in the incubator
it goes and finds another one
What happens to the enzyme when it catalyzes reactant
iodine
What indication was used to check for starch diffusing out
over .9
What percent of solute would be hypertonic to red blood cells
The water would create a steep gradient which would cause the blood vessels to pop
What would happen if you were given pure water in an IV?
it increases the motion of molecules and therefore the number of times the enzyme and substrate interact
Why does a warm temperature promote enzyme activity?
the active site must have a shape complementary to the substrate for the reaction to occur
Why does enzyme specificity promote enzyme activity?
It increases the number of active sites available
Why does increasing enzyme concentration promote enzyme activity?
optimum pH is required to maintain the active site of an enzyme
Why does optimum pH promote enzyme activity?
water
_________ was my negative control for my macromolecules lab experiments
Homeostasis
________________ is the maintenance of internal balance in the body for conditions such as temperature and pH
reactants
are the substrates in an enzymatic reaction reactants or products
nucleus
as opposed to the eukaryotic cell, the prokaryotic cell has no....
prokaryotic bc it is more simplistic
based on their structure, is it likely that the prokaryotic or eukaryotic cell evolved first? why
Inhibitors (competitive)
block substrates by blocking the active site
denature
boiling causes enzymes to...
catalase
breaks down hydrogen peroxide into water and oxygen
into
does water move into or out of cells that are placed in a hypotonic solution
optimum
enzymes work best at their _________pH
it denatures
how does pH affect the structure of an enzyme
increase
if enzyme concentration increases, will products from the enzyme reaction increase, decrease, or stay the same
tube 1
in the experimental procedure on the effect of the pH on enzyme activity, which tube contained water with an acidic pH
Monosaccharide test
in the macromolecules lab, Benedicts reagent is used to test for sugars. In the presence of simple sugars benedicts will turn orange, red or yellow. what test is this
carb-starch test
in the macromolecules lab, In the presence of starches (polysaccharides) iodine turns blackish-purple. what test is this
protein test
in the macromolecules lab, the biuret solution is blue and turns pink or purple in the presence of proteins. what test was this
lipid test
in the macromolecules lab, you looked for a grease spot on a brown piece of paper. what test was this
Substrate
reactant of an enzyme-catalyzed reaction
change colors
the starch stayed in tubing because the iodine did not...
plasma membrane, cytoplasm, dna, ribosomes
what cellular components are common to prokaryotes and eukaryotes
peroxisome
what cellular organelle contains catalase and is present in all plant and animal organs
enzyme activity
what did measuring the bubble column heights in all the experimental procedures indicate
catalase
what did the control tube lack when the effect of enzyme concentration was studied
mitochondria
what energy (ATP) prodding organelles is found in animal and plants cells?
activity increases
what happens to enzyme activity if the temperature increases a few degrees
active site
what is the name of the location on an enzyme where its substrate or substrates bind
hypotonic
what kind of solution cause chloroplasts to be pushed outward against the cell wall? (hint: expanding and bursting)
hypertonic
what kind of solution causes crenation to happen to red blood cells
cellulose
what molecule is associated with plant cell walls
Chloroplast
what plant cell organelle carries out photosynthesis?
negative control
what purpose did the tube containing water and hydrogen peroxide serve in the catalase activity experimental procedure
plasma membrane
what regulates the movement of molecules into and out of the cytoplasm?
ribosomes
what structures associated with prokaryotic and eukaryotic cells carry out proteins synthesis?
dna, rna
what two things are found in the nucleus of a eukaryotic cell
denature
what word describes the loss of an enzyme's original shape and ability of its active site to function
arrow
which symbol in a chemical reaction represents the change from reactants to products