bio 1001: lab practical 2

plasma membrane

(cell lab) the dialysis tubing in the experimental procedure stimulates a....

starch did not diffuse bc the molecules are too large to diffuse from the tubing

(cell lab) which solute did not diffuse across the dialysis membrane from the bag to the beaker? how do you know?

more bubbles

According to test results what happened when you increase substrates

Gluclose

Based on results of Benedict's test, what solute diffused across the dialysis membrane from the bag to the beaker?

water and oxygen

Catalayse takes H2O2 and turns it into....

It maintains the lens parameters and strength of the solution

Contact lens solution is described as sterile, buffered, isotonic aqueos solution. Explain the importance of the adjectives buffered and isotonic to the person buying the solution

osmosis

Diffusion of water through a selectively permeable membrane

diffusion

Movement of molecules from an area of higher concentration to an area of lower concentration.

halose

New enzyme breaks down tree halase

There is an an abundance of Smooth ER

Ovarian follicle cells produce estrogen, which is a steroid. What organelle will be present in abundance so that the follicle cells might perform this funciton?

Degradation Enzymatic Reaction

Since enzymes are specific for certain substrates and often have similar names, what is the name of the enzyme used in tube 3 of the catalase activity experiment that facilitates the breakdown of sucrose?

Activation energy

The enzymes lowers this energy. what does it lower- ___________________ (barrier)

Nucleoid

What are the organelles that are specific to a bacterial cell?

affects hydrogen bonding and structure of the enzyme

What do changes in pH (too high or too low) do to an enzyme that reduces its activity

warms up should have increased activity

What happened when you put it in the incubator

it goes and finds another one

What happens to the enzyme when it catalyzes reactant

iodine

What indication was used to check for starch diffusing out

over .9

What percent of solute would be hypertonic to red blood cells

The water would create a steep gradient which would cause the blood vessels to pop

What would happen if you were given pure water in an IV?

it increases the motion of molecules and therefore the number of times the enzyme and substrate interact

Why does a warm temperature promote enzyme activity?

the active site must have a shape complementary to the substrate for the reaction to occur

Why does enzyme specificity promote enzyme activity?

It increases the number of active sites available

Why does increasing enzyme concentration promote enzyme activity?

optimum pH is required to maintain the active site of an enzyme

Why does optimum pH promote enzyme activity?

water

_________ was my negative control for my macromolecules lab experiments

Homeostasis

________________ is the maintenance of internal balance in the body for conditions such as temperature and pH

reactants

are the substrates in an enzymatic reaction reactants or products

nucleus

as opposed to the eukaryotic cell, the prokaryotic cell has no....

prokaryotic bc it is more simplistic

based on their structure, is it likely that the prokaryotic or eukaryotic cell evolved first? why

Inhibitors (competitive)

block substrates by blocking the active site

denature

boiling causes enzymes to...

catalase

breaks down hydrogen peroxide into water and oxygen

into

does water move into or out of cells that are placed in a hypotonic solution

optimum

enzymes work best at their _________pH

it denatures

how does pH affect the structure of an enzyme

increase

if enzyme concentration increases, will products from the enzyme reaction increase, decrease, or stay the same

tube 1

in the experimental procedure on the effect of the pH on enzyme activity, which tube contained water with an acidic pH

Monosaccharide test

in the macromolecules lab, Benedicts reagent is used to test for sugars. In the presence of simple sugars benedicts will turn orange, red or yellow. what test is this

carb-starch test

in the macromolecules lab, In the presence of starches (polysaccharides) iodine turns blackish-purple. what test is this

protein test

in the macromolecules lab, the biuret solution is blue and turns pink or purple in the presence of proteins. what test was this

lipid test

in the macromolecules lab, you looked for a grease spot on a brown piece of paper. what test was this

Substrate

reactant of an enzyme-catalyzed reaction

change colors

the starch stayed in tubing because the iodine did not...

plasma membrane, cytoplasm, dna, ribosomes

what cellular components are common to prokaryotes and eukaryotes

peroxisome

what cellular organelle contains catalase and is present in all plant and animal organs

enzyme activity

what did measuring the bubble column heights in all the experimental procedures indicate

catalase

what did the control tube lack when the effect of enzyme concentration was studied

mitochondria

what energy (ATP) prodding organelles is found in animal and plants cells?

activity increases

what happens to enzyme activity if the temperature increases a few degrees

active site

what is the name of the location on an enzyme where its substrate or substrates bind

hypotonic

what kind of solution cause chloroplasts to be pushed outward against the cell wall? (hint: expanding and bursting)

hypertonic

what kind of solution causes crenation to happen to red blood cells

cellulose

what molecule is associated with plant cell walls

Chloroplast

what plant cell organelle carries out photosynthesis?

negative control

what purpose did the tube containing water and hydrogen peroxide serve in the catalase activity experimental procedure

plasma membrane

what regulates the movement of molecules into and out of the cytoplasm?

ribosomes

what structures associated with prokaryotic and eukaryotic cells carry out proteins synthesis?

dna, rna

what two things are found in the nucleus of a eukaryotic cell

denature

what word describes the loss of an enzyme's original shape and ability of its active site to function

arrow

which symbol in a chemical reaction represents the change from reactants to products