Blood Banking Chapter 2 Homework
Reagent to identify the specificity of a red cell antibody antisera
Panel Cells
In the solid-phase red cell adherence test, a negative test would appear as
a button of cells on the bottom of the well
Polyspecific antihuman globulin contains
anti-IgG and anti-C3d
Solid-phase red cell adherence used for antibody detection has an advantage over tube testing because
the endpoint is more clearly defined
Following centrifugation of the gel card, red cells are observed to be evenly dispersed throughout one of the microtubes. This reaction could be graded as a
2+
The indirect antihuman globulin test is incubated at what temperature
37C
Reagent used in the identification of ABO antibodies
A1 and B cells
Reagent used to determine the ABO antigenic composition of a patient's red cells
ABO antisera
Detects IgG or complement-coated red cells
Both the direct and indirect antiglobulin test
Requires washing the cells several times before the addition of antihuman globulin reagent
Both the direct and indirect antiglobulin test
Incubation step is not necessary
Direct antiglobulin test
which red cell antigens do proteolytic enzymes destroy
Fya and Fyb in the Duffy system
What immunoglobulin class reacts best by antiglobulin testing
IgG
Tests for certain clinical conditions such as hemolytic disease of the newborn and autoimmune hemolytic anemia
Indirect antiglobulin test
Reagent derived from plants used to distinguish group A1 from group A2 red cells
Lectins
The antiglobulin test was performed using gel technology. A button of cells was observed at the bottom of the microtube following centrifugation. This result indicates a
Negative reaction
Reagent to detect the presence of red cell antibodies and B cells
Screening Cells
The evidence for reagent red cell deterioration may include
Spontaneous agglutination, significant hemolysis, loss of agglutination strength over time
Rouleaux is a false-positive reaction that would not likely be observed during which of the following tests
Weak D test
Not an advantage of using a monoclonal antibody over a polyclonal antibody
all variations of the antigen can be detected
Which test that uses IgG-sensitized red cells (check cells)
antiglobulin test
The purpose of adding antibody-sensitized red cells following the antiglobulin test is to
check that the wash procedure was sufficient to remove unbound antibodies
. Which method uses a dextran-acrylamide matrix
gel technology
The gel technology method uses a concentration of red cells that is
lower than tube techniques.
Reagent antibodies prepared from human sources are
polyclonal in specificity
What reagent contains antibodies to multiple antigenic epitopes
polyclonal-based
Which red cells are used to screen for antibodies in donor samples
pooled screen cells
Product limitations and technical considerations for each reagent can be found in
product insert
Why is incubation omitted in the direct antihuman globulin test
the antigen-antibody complex has already formed in vivo
Advantage of using a monoclonal antibody over a polyclonal antibody
there are very few variations between lots, there are no contaminating antibodies, direct agglutination is usually faster.
Which of the following statements is true regarding high-protein anti-D reagents
they have been largely replaced with low-protein monoclonal reagents, they contain approximately 20% bovine albumin, they may increase the possibility of a false-positive reaction, requiring the use of a control.
Which of the following statements is true regarding IgG-sensitized red cells
they must be used to confirm a negative antiglobulin tube test
How would you interpret the results if both the anti-D reagent and the Rh control were 2+ agglutination reactions
unable to determine without further testing
Monoclonal antibodies are prepared in
vitro