Blood Banking Chapter 2 Homework

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Reagent to identify the specificity of a red cell antibody antisera

Panel Cells

In the solid-phase red cell adherence test, a negative test would appear as

a button of cells on the bottom of the well

Polyspecific antihuman globulin contains

anti-IgG and anti-C3d

Solid-phase red cell adherence used for antibody detection has an advantage over tube testing because

the endpoint is more clearly defined

Following centrifugation of the gel card, red cells are observed to be evenly dispersed throughout one of the microtubes. This reaction could be graded as a

2+

The indirect antihuman globulin test is incubated at what temperature

37C

Reagent used in the identification of ABO antibodies

A1 and B cells

Reagent used to determine the ABO antigenic composition of a patient's red cells

ABO antisera

Detects IgG or complement-coated red cells

Both the direct and indirect antiglobulin test

Requires washing the cells several times before the addition of antihuman globulin reagent

Both the direct and indirect antiglobulin test

Incubation step is not necessary

Direct antiglobulin test

which red cell antigens do proteolytic enzymes destroy

Fya and Fyb in the Duffy system

What immunoglobulin class reacts best by antiglobulin testing

IgG

Tests for certain clinical conditions such as hemolytic disease of the newborn and autoimmune hemolytic anemia

Indirect antiglobulin test

Reagent derived from plants used to distinguish group A1 from group A2 red cells

Lectins

The antiglobulin test was performed using gel technology. A button of cells was observed at the bottom of the microtube following centrifugation. This result indicates a

Negative reaction

Reagent to detect the presence of red cell antibodies and B cells

Screening Cells

The evidence for reagent red cell deterioration may include

Spontaneous agglutination, significant hemolysis, loss of agglutination strength over time

Rouleaux is a false-positive reaction that would not likely be observed during which of the following tests

Weak D test

Not an advantage of using a monoclonal antibody over a polyclonal antibody

all variations of the antigen can be detected

Which test that uses IgG-sensitized red cells (check cells)

antiglobulin test

The purpose of adding antibody-sensitized red cells following the antiglobulin test is to

check that the wash procedure was sufficient to remove unbound antibodies

. Which method uses a dextran-acrylamide matrix

gel technology

The gel technology method uses a concentration of red cells that is

lower than tube techniques.

Reagent antibodies prepared from human sources are

polyclonal in specificity

What reagent contains antibodies to multiple antigenic epitopes

polyclonal-based

Which red cells are used to screen for antibodies in donor samples

pooled screen cells

Product limitations and technical considerations for each reagent can be found in

product insert

Why is incubation omitted in the direct antihuman globulin test

the antigen-antibody complex has already formed in vivo

Advantage of using a monoclonal antibody over a polyclonal antibody

there are very few variations between lots, there are no contaminating antibodies, direct agglutination is usually faster.

Which of the following statements is true regarding high-protein anti-D reagents

they have been largely replaced with low-protein monoclonal reagents, they contain approximately 20% bovine albumin, they may increase the possibility of a false-positive reaction, requiring the use of a control.

Which of the following statements is true regarding IgG-sensitized red cells

they must be used to confirm a negative antiglobulin tube test

How would you interpret the results if both the anti-D reagent and the Rh control were 2+ agglutination reactions

unable to determine without further testing

Monoclonal antibodies are prepared in

vitro


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