CHAPTER 14
The anticodon loop of the first tRNA that will complement this mRNA is
3' GGC 5'.
A particular triplet of bases in the template strand of DNA is 5' AGT 3'. The corresponding codon for the mRNA transcribed is
3' UCA 5'.
Which of the following does not occur in prokaryotic gene expression, but does occur in eukaryotic gene expression?
A polyA tail is added to the 3' end of an mRNA and a cap is added to the 5' end.
Why might a point mutation in DNA make a difference in the level of a protein's activity?
It might substitute a different amino acid in the active site.
During splicing, which molecular component of the spliceosome catalyzes the excision reaction?
RNA
the figure represents tRNA that recognizes and binds a particular amino acid (in this instance, phenylalanine). Which codon on the mRNA strand codes for this amino acid?
UUC
In order for a eukaryotic gene to be engineered into a bacterial colony to be expressed, what must be included in addition to the coding exons of the gene?
a bacterial promoter sequence
Accuracy in the translation of mRNA into the primary structure of a polypeptide depends on specificity in the
bonding of the anticodon to the codon and the attachment of amino acids to tRNAs
What amino acid sequence will be generated, based on the following mRNA codon sequence? 5' AUGUCUUCGUUAUCCUUG 3'
met-ser-ser-leu-ser-leu
The dipeptide that will form will be
prolinethreonine.
Transcription in eukaryotes requires which of the following in addition to RNA polymerase?
several transcription factors
Which of the following is the first event to take place in translation in eukaryotes?
the small subunit of the ribosome recognizes and attaches to the 5' cap of mRNA