CHAPTER 14

The anticodon loop of the first tRNA that will complement this mRNA is

3' GGC 5'.

A particular triplet of bases in the template strand of DNA is 5' AGT 3'. The corresponding codon for the mRNA transcribed is

3' UCA 5'.

Which of the following does not occur in prokaryotic gene expression, but does occur in eukaryotic gene expression?

A polyA tail is added to the 3' end of an mRNA and a cap is added to the 5' end.

Why might a point mutation in DNA make a difference in the level of a protein's activity?

It might substitute a different amino acid in the active site.

During splicing, which molecular component of the spliceosome catalyzes the excision reaction?

RNA

the figure represents tRNA that recognizes and binds a particular amino acid (in this instance, phenylalanine). Which codon on the mRNA strand codes for this amino acid?

UUC

In order for a eukaryotic gene to be engineered into a bacterial colony to be expressed, what must be included in addition to the coding exons of the gene?

a bacterial promoter sequence

Accuracy in the translation of mRNA into the primary structure of a polypeptide depends on specificity in the

bonding of the anticodon to the codon and the attachment of amino acids to tRNAs

What amino acid sequence will be generated, based on the following mRNA codon sequence? 5' AUGUCUUCGUUAUCCUUG 3'

met-ser-ser-leu-ser-leu

The dipeptide that will form will be

prolinethreonine.

Transcription in eukaryotes requires which of the following in addition to RNA polymerase?

several transcription factors

Which of the following is the first event to take place in translation in eukaryotes?

the small subunit of the ribosome recognizes and attaches to the 5' cap of mRNA