Chapter 18

63. The formation and breakdown of fructose 2,6-bisphosphate a. is catalyzed by the same protein dependant upon whether it is phosphorylated or not. b. is catalyzed by the same protein dependant allosteric regulators. c. is catalyzed by different proteins. d. none of these

. is catalyzed by the same protein dependant upon whether it is phosphorylated or not.

80. Which of the following molecules does not directly regulate the activity of glycogen synthase? a. Glucose b. Glucose-6-phosphate c. AMP d. ATP e. Concentration of all of these affects glycogen synthase.

a. Glucose

40. Which enzyme is used in gluconeogenesis, but NOT in glycolysis? a. PEP carboxykinase b. enolase c. phosphohexose isomerase d. 3-phosphoglycerate dehydrogenase

a. PEP carboxykinase

. Properly used, glycogen loading by athletes is a safe process. a. True b. False

a. True

22. Control of the entry of glucose or fructose into the oxidative portions of the pentose phosphate pathway is mostly dependent on a cell's need for NADPH, rather than on the cell's need for pentoses. a. True b. False

a. True

24. Generally speaking, the same mechanisms that activate glycogen phosphorylase will turn off glycogen synthase. a. True b. False

a. True

33. In general, opposing pathways, such as glycolysis and gluconeogenesis are not exact reversals of each other. a. True b. False

a. True

43. Hydrolysis of pyrophosphate is an important energy driving force in the synthesis of glycogen. a. True b. False

a. True

6. The same enzymes are responsible for covalent modifications of both glycogen synthase and glycogen phosphorylase in regulating their activity. a. True b. False

a. True

68. Glucose-6-phosphatase activity is found associated with the endoplasmic reticulum. a. True b. False

a. True

8. It is impossible to reverse any kinase reaction under physiological conditions. a. True b. False

a. True

44. Glycogen synthase and glycogen phosphorylase a. are modified by the same enzymes b. are not subject to allosteric control c. are not subject to covalent modification d. none of these

a. are modified by the same enzymes

77. Glycogen phosphorylase and glycogen synthase a. are not activated simultaneously b. are activated simultaneously under special circumstances c. can be activated simultaneously under any circumstances d. are involved in catalysis of the same process

a. are not activated simultaneously

70. Gluconeogenesis is the synthesis of a. glucose from non-carbohydrate precursors b. glycogen from glucose c. pyruvate from glucose d. fatty acids from glucose e. glucose from glycogen

a. glucose from non-carbohydrate precursors

65. Which of the following happens when epinephrine and glucagon are released a. glycogen phosophorylase is activated and glycogen synthase is inhibited b. glycogen phosphorylase and glycogen synthase are activated c. glycogen phosphorylase and glycogen synthase are inhibited d. glycogen phosphorylase is inhibited and glycogen synthase is activated e. none of these

a. glycogen phosophorylase is activated and glycogen synthase is inhibited

39. When glycogen synthase is phosphorylated a. its activity decreases. b. its activity increases. c. its activity is unaffected. d. none of these because that enzyme doesn't get phosphorylated.

a. its activity decreases.

79. Glycogen is mainly found in a. liver and muscle. b. liver and brain. c. muscle and brain. d. liver, muscle, and brain. e. adipose tissue

a. liver and muscle.

16. In gluconeogenesis, the initial reaction converts pyruvate to a. oxaloacetate b. acetyl-CoA c. phosphoenolpyruvate d. lactate e. glucose

a. oxaloacetate

45. The oxidative reactions of the pentose phosphate pathway a. produce NADPH rather than NADH. b. require biotin. c. require coenzyme A. d. require thiamine pyrophosphate.

a. produce NADPH rather than NADH.

66. High concentrations of fructose-2,6-bisphopshate a. stimulate glycolysis and inhibit gluconeogenesis. b. inhibit glycolysis and stimulate gluconeogenesis. c. stimulate both glycolysis and gluconeogenesis. d. inhibit both glycolysis and gluconeogenesis. e. none of these

a. stimulate glycolysis and inhibit gluconeogenesis.

62. Of the various forms of glycogen phosphorylase, the most active would be: a. the phosphorylated R form b. the unphosphorylated R form c. the phosphorylated T form d. the unphosphorylated T form e. all of the forms have the same activity

a. the phosphorylated R form

51. A characteristic of the reaction catalyzed by transaldolase is a. transfer of a three-carbon unit b. transfer of a two-carbon unit c. a requirement for TPP as a coenzyme d. an energy requirement for oxidative decarboxylation

a. transfer of a three-carbon unit

A characteristic of the reaction catalyzed by transaldolase is a. transfer of a three-carbon unit b. transfer of a two-carbon unit c. a requirement for TPP as a coenzyme d. an energy requirement for oxidative decarboxylation

a. transfer of a three-carbon unit

15. What is the average chain length of the branches in glycogen? a. 6 glucose residues b. 13 glucose residues c. 25 glucose residues d. 50 glucose residues e. 100 glucose residues

b. 13 glucose residues

75. Starting from glucose and UTP and ATP, how many high-energy bonds are broken/consumed to add that glucose to a glycogen molecule? a. 1 b. 2 c. 3 d. 4 e. The answer cannot be determined form the information given.

b. 2

7. The enzyme glucose-6-phosphatase is only found in cells which have this function or ability: a. Ability to utilize glucose anaerobically. b. Ability to replenish the levels of glucose in the blood. c. Glycogen storage. d. Ability to produce lactic acid as an end product of metabolism. e. Glucose-6-phosphatase activity is found in almost all types of cells.

b. Ability to replenish the levels of glucose in the blood.

81. Which of the following statements concerning branched polymers like glycogen is false? a. Branched polymers are more accessible to enzymes since they bond more water. b. All of the reducing ends of the branched polymer are available to release glucose. c. All of the non-reducing ends of the branched polymer are available to release glucose. d. A branched polymer like glycogen is more compact and stores more glucose molecules in a given volume.

b. All of the reducing ends of the branched polymer are available to release glucose.

61. All of the following sugar rearrangements are part of the pentose phosphate pathway, except. a. C5 + C5 → C7 + C3 b. C5 + C5 → C6 + C4 c. C7 + C3 → C6 + C4 d. C5 + C4 → C6 + C3 e. All of these rearrangements occur in the pentose phosphate pathway.

b. C5 + C5 → C6 + C4

5. Which of the following statements concerning biotin and gluconeogenesis is false? a. Biotin is used to add CO2 to certain intermediates in gluconeogenesis. b. CO2 is incorporated into the glucose product. c. Biotin is capable of binding covalently to CO2. d. Biotin helps synthesize an important precursor of phosphoenolpyruvate. e. ATP hydrolysis is required to attach CO2 to biotin.

b. CO2 is incorporated into the glucose product.

18. The liver contains a special enzyme, hexokinase, to act as a backup for glucokinase when the glucose levels in the body get very high. a. True b. False

b. False

47. Glycogen loading is particularly advantageous for providing energy for long distance athletic events, such as running the marathon. a. True b. False

b. False

54. There is as much energy used to add a phosphate group by means of phosphorolysis, as the energy required adding a phosphate using ATP. a. True b. False

b. False

78. In one normal cycle of the pentose phosphate pathway, the enzyme transaldolase is used twice and the enzyme transketolase is used once. a. True b. False

b. False

Branching and debranching enzymes use the exact same mechanism to add and remove the branches of the glycogen polymer. a. True b. False

b. False

23. Which nucleotide triphosphate is hydrolyzed in the conversion of oxaloacetate to phosphoenolpyruvate? a. ATP b. GTP c. CTP d. UTP e. No nucleotide triphosphate is hydrolysed.

b. GTP

34. UDP-glucose pyrophosphorylase works by this mechanism: a. It adds a phosphate group to glucose from UTP, leaving behind UDP. b. It adds a UMP molecule to glucose-1-phosphate by splitting out pyrophosphate. c. It adds a pyrophosphate group to glucose, using UTP. d. It adds a UDP molecule to glucose by splitting out phosphate. e. None of these

b. It adds a UMP molecule to glucose-1-phosphate by splitting out pyrophosphate.

67. The NADH used for the reduction reactions during gluconeogenesis usually come from this reaction: a. Gylceraldehyde-3-phosphate dehydrogenase. b. Malate dehydrogenase activity in the cytoplasm. c. Pyruvate carboxylase activity in the mitochondria. d. A variety of reactions which produce NADH in the mitochondria. e. None of these, since it is NAD+ that is used in gluconeogenesis.

b. Malate dehydrogenase activity in the cytoplasm.

14. The first reaction of gluconeogenesis is a. an oxidative decarboxylation b. a carboxylation c. a redox reaction d. a phosphate transfer

b. a carboxylation

21. Which enzyme cleaves the α(1 → 6) bonds in glycogen? a. glycogen phosphorylase b. debranching enzyme c. phosphoglucomutase d. glycogen synthase e. None of these because there are no α(1 → 6) bonds in glycogen.

b. debranching enzyme

71. What kind of enzyme catalyzes the reaction ribulose-5-P ↔ xylulose-5-P? a. transketolase b. epimerase c. transaldolase d. isomerase

b. epimerase

59. The enzyme glycogen phosphorylase catalyzes a reaction in a. the formation of glycogen from glucose. b. glycogen breakdown. c. gluconeogenesis. d. the pentose phosphate pathway. e. glycolysis

b. glycogen breakdown.

10. The compound uridine diphosphate glucose (UDPG) plays a role in a. glycogen breakdown. b. glycogen synthesis. c. glycolysis. d. gluconeogenesis. e. none of these

b. glycogen synthesis.

19. When glycogen phosphorylase is phosphorylated a. its activity decreases. b. its activity increases. c. its activity is unaffected. d. none of these because that enzyme doesn't get phosphorylated.

b. its activity increases.

49. In which cellular compartment is pyruvate carboxylase found? a. cytosol b. mitochondria c. endoplasmic reticulum d. nucleus

b. mitochondria

50. What kind of reaction is used to release glucose units from glycogen? a. hydrolysis b. phosphorolysis c. dehydration d. oxidation e. dehydrogenation

b. phosphorolysis

46. The enzyme pyruvate carboxylase a. is not subject to allosteric control b. requires biotin for activity c. catalyzes a reaction that does not require ATP d. is inhibited by acetyl-CoA

b. requires biotin for activity

36. How many ATP equivalents are expended to convert 2 pyruvates to 1 glucose? a. 2 b. 4 c. 6 d. 8

c. 6

42. Which of the following is true? a. Gluconeogenesis from pyruvate involves simple reversal of all of the glycolysis reactions, except for the reactions that consumed ATP during catabolism. b. Anabolic reactions are usually the reversal of catabolic ones c. In gluconeogenesis, the effective reversal of the glycolytic reactions of hexokinase and phosphofructokinase involve the same sugar molecules but not the exact set of substrates and products d. all of these are true

c. In gluconeogenesis, the effective reversal of the glycolytic reactions of hexokinase and phosphofructokinase involve the same sugar molecules but not the exact set of substrates and products

26. Glycolysis that starts with glycogen instead of glucose can be considered to have a higher energy yield because: a. Phosphorolysis reactions cleave bonds with phosphate instead of water. b. Phosphorylase is a better enzyme than hexokinase c. Phosphorylase produces a glucose phosphate without spending an ATP to do it d. All of these e. None of these is true because glycolysis starting from glycogen does not have a higher energy yield

c. Phosphorylase produces a glucose phosphate without spending an ATP to do it

37. The process called substrate cycling is used to describe this process: a. Freely reversible reactions. b. Recycling of vitamins as catalysts in metabolism c. Situations where there are two different enzymes used to reverse a specific step in a metabolic pathway. d. Metabolic pathways which run in cycles and regenerate the initial molecule. e. None of these answers describes substrate cycling.

c. Situations where there are two different enzymes used to reverse a specific step in a metabolic pathway.

60. Which of the following is not a control mechanism for glycogen phosphorylase? a. Covalent modification. b. Inhibition by glucose. c. Subunit association and dissociation. d. Allosteric stimulation by AMP. e. All of these mechanisms regulate the activity of glycogen synthase.

c. Subunit association and dissociation.

82. The enzyme that catalyzes the interconversion of glucose-1-phosphate and glucose-6-phosphate is a. a hydrolase. b. a phosphorylase. c. a mutase. d. a dehydrogenase. e. an epimerase

c. a mutase.

48. The activity of glycogen phosphorylase depends on a. allosteric control b. covalent modification c. both of these d. neither of these

c. both of these

9. Glycogen phosphorylase a. exists in two forms, phosphorylase a and phosphorylase b b. responds differently to allosteric effectors in its phosphorylated and dephosphorylated forms c. both of these d. neither of these

c. both of these

57. Where is the enzyme glucose-6-phosphatase located? a. cytosol b. mitochondria c. endoplasmic reticulum d. nucleus e. none of these

c. endoplasmic reticulum

69. Which of the following hormone's main function is to raise the level of blood glucose? a. epinephrine b. insulin c. glucagon d. none of these raises blood glucose

c. glucagon

74. The regulatory enzymes in gluconeogenesis are a. hexokinase, phosphofructokinase, and pyruvate kinase b. glucose-6-kinase, aldolase, and enolase c. glucose-6-phosphatase, fructose-1,6-bisphosphatase, pyruvate carboxylase, and phosphoenolpyruvate carboxykinase d. pyruvate carboxylase, aldolase, and phosphofructokinase

c. glucose-6-phosphatase, fructose-1,6-bisphosphatase, pyruvate carboxylase, and phosphoenolpyruvate carboxykinase

38. Which of the following molecules is not a product of the pentose phosphate pathway? a. NADPH b. ribose-5-phosphate c. glycerate-3-phosphate d. xylulose-5-phosphate

c. glycerate-3-phosphate

53. The activity of the enzyme fructose 1,6-bisphosphatase is a. inhibited by ATP. b. stimulated by AMP. c. inhibited by fructose 2,6-bisphosphate. d. not subject to allosteric control.

c. inhibited by fructose 2,6-bisphosphate.

20. If you're running away from a bear, a. both your liver cells and my leg muscle cells will be running glycolysis. b. both your liver cells and my leg muscle cells will be running gluconeogenesis. c. your liver cells will be running gluconeogenesis and your leg muscle cells will be running glycolysis. d. your liver cells will be running glycolysis and your leg muscle cells will be running gluconeogenesis.

c. your liver cells will be running gluconeogenesis and your leg muscle cells will be running glycolysis

17. How are the branches in glycogen produced? a. A branching enzyme catalyses the addition of a single glucose unit via an α(1,6) bond to glycogen from a glucose-1-phosphate, releasing Pi. b. A branching enzyme catalyses the addition of a single glucose unit via an α(1,6) bond to glycogen from a glucose-6-phosphate, releasing Pi. c. A branching enzyme catalyses the addition of a single glucose unit via an α(1,6) bond to glycogen from a glucose-UDP, releasing UDP. d. A branching enzyme moves a short chain of several glucose units from a linear α(1,4) section of glycogen to make a new α(1,6) branch point.

d. A branching enzyme moves a short chain of several glucose units from a linear α(1,4) section of glycogen to make a new α(1,6) branch point.

76. Which of the following reactions occur when a single glucose residue is transferred from UDP-glucose to a growing glycogen molecule? a. UDP is released. b. The glucose can be attached to a #4 carbon atom in the glycogen molecule. c. UDP is released and the glucose is attached to a #4 carbon. d. All of these events can occur when a single glucose residue is added.

d. All of these events can occur when a single glucose residue is added.

2. The Cori cycle involves the following: a. Conversion of lactate produced in the liver by regeneration of glucose in the muscle. b. Conversion of pyruvate produced in the muscle by regeneration of glucose in the liver. c. Conversion of lactate produced in the muscle by regeneration of glucose in the liver. d. Conversion of NADH produced in the muscle by regeneration of glucose in the liver. e. The Cori cycle involves a different pathway from any of these.

d. Conversion of NADH produced in the muscle by regeneration of glucose in the liver.

3. Which of the following is not an advantage that glycogen provides to muscle cells in which it is stored? a. It is available for quick energy spurts. b. It requires no energy to mobilize the glucose residues for metabolism. c. It gives anaerobic metabolism a boost. d. It draws more water into the cells than glucose would. e. All of these are advantages that glycogen provides to muscle cells.

d. It draws more water into the cells than glucose would.

64. Which of the following best describes how liver supplies other tissues with the glucose it releases from its stored glycogen? a. It releases short chains of glucose residues into the blood. b. It releases glucose-1-phosphate into the blood. c. It releases glucose-6-phosphate into the blood. d. It releases glucose into the blood. e. It releases glucose in the form of lipopolysaccharides

d. It releases glucose into the blood.

12. Which of the following best describes the function of debranching enzyme? a. It simply cleaves α(1 → 6) bonds in glycogen via phosphorolysis. b. It transfers a set of three glucose residues from a limit branch and then cleaves the α(1 → 6) bond via phosphorylsis. c. It simply cleaves α(1 → 6) bonds in glycogen via hydrolysis. d. It transfers a set of three glucose residues from a limit branch and then cleaves the α(1 → 6) bond via hydrolysis. e. none of these describes the enzyme's function

d. It transfers a set of three glucose residues from a limit branch and then cleaves the α(1 → 6) bond via hydrolysis.

41. Which of the following statements concerning the initial phase of the pentose phosphate pathway is false? a. A CO2 molecule is released from glucose. b. Two molecules of NADPH are produced per glucose. c. Ribulose-5-phosphate is produced by an oxidative decarboxylation of 6-phosphogluconate. d. The process reduces glucose-6-phosphate.

d. The process reduces glucose-6-phosphate.

35. Gluconeogenesis differs from glycolysis because a. the irreversible steps of glycolysis are bypassed b. different enzymes are involved c. biotin is required for gluconeogenesis and not for glycolysis d. all of these e. none of these

d. all of these

56. Hemolytic anemia is associated with the pentose phosphate pathway because: a. a deficiency of this pathway leads to a lack of NADPH in red blood cells b. NADPH is required to reduce glutathione c. Red blood cells have minimal resources for maintaining an oxidative balance d. all of these

d. all of these

29. In the process of substrate cycling a. different organs are responsible for the synthesis and breakdown of the same substance. b. there is no net consumption of ATP. c. allosteric control is not involved. d. different enzymes, subject to independent control, are responsible for the synthesis and breakdown of the same substance.

d. different enzymes, subject to independent control, are responsible for the synthesis and breakdown of the same substance.

72. The molecule fructose 2,6-bisphosphate: a. is an intermediate in glycolysis b. is an intermediate of gluconeogenesis but not glycolysis c. does not exist d. is an important regulatory molecule in the control of carbohydrate metabolism e. none of these

d. is an important regulatory molecule in the control of carbohydrate metabolism

73. What kind of enzyme catalyzes the following reaction? ribose-5-P ↔ ribulose-5-P a. transketolase b. epimerase c. transaldolase d. isomerase

d. isomerase

4. The enzyme phosphopentose isomerase is characterized by all the following except a. it catalyzes the interconversion of ribose-5-phosphate and ribulose-5-phosphate b. there is no requirement for ATP c. it converts a ketose to an aldose d. it catalyzes an inversion of configuration at carbon-3

d. it catalyzes an inversion of configuration at carbon-3

31. Which of the following is NOT a hormone involved in the control of carbohydrate metabolism? a. epinephrine b. glucagon c. insulin d. phosphofructokinase

d. phosphofructokinase

28. If an individual lacked the debranching enzyme, the effect would be: a. the individual could not make glycogen b. the individual could make glycogen but not store it c. the individual would not be able to utilize any glucose from glycogen d. the individual would not be able to completely break down a glycogen molecule e. none of these

d. the individual would not be able to completely break down a glycogen molecule

27. Which of the following mechanisms can be used to regulate metabolic pathways? a. Allosteric activators and inhibitors. b. Covalent modifications of enzymes. c. Use of separate enzymes at a given point in the forward and backward pathways. d. Regulation of the genes for the enzymes used in the pathway. e. All of these are used to regulate metabolism.

e. All of these are used to regulate metabolism.

55. In addition to pentoses, the pentose phosphate pathway involves sugars of all these sizes except: a. 3 carbons b. 4 carbons c. 6 carbons d. 7 carbons e. All of these sizes are used in this pathway.

e. All of these sizes are used in this pathway.

58. During the pentose phosphate pathway (glucose-6-P → ribose-5-P), is there a net oxidation of the substrate carbon atoms? a. Yes. b. No. c. It depends on whether the process is under aerobic or anaerobic conditions. d. It depends on the species doing the process. e. It depends on whether the glucose goes through the oxidative part of the pathway or not.

e. It depends on whether the glucose goes through the oxidative part of the pathway or not.

30. The vitamin thiamine is important in transferring all of these types of groups, except: a. 2-carbon sugar fragments b. 3-carbon sugar fragments c. 4-carbon sugar fragments d. Sugar fragments which contain a carbonyl group (C=O). e. Thiamine can transfer all of these types of groups.

e. Thiamine can transfer all of these types of groups.

Which of the following occurs when insulin is released? a. insulin binds to receptors on cell surfaces b. a protein kinase cascade is begun that leads to glycogen synthesis c. insulin stimulates the GLUT4 transport protein system in muscle cells d. glucose transporters move glucose out of the blood and into the cell e. all of these

e. all of these