Chapter 5 Review Questions

The steps involved in the Southern Blot test should be performed in the following order 1 = x-ray film 2 = electrophoresis 3 = digestion with restriction enzyme 4 = ethidium bromide 5 = radioactive probe A) 3, 2, 4, 5, 1 B) 3, 4, 2, 5, 1 C) 3, 2, 5, 4, 1 D) 2, 4, 3, 5, 1

A) 3, 2, 4, 5, 1

For DNA probes to hybridize A) DNA strands must be homologous, having regions with similar or identical nucleotide sequences B) DNA strands must be non-homologous C) DNA must be complementary to RNA D) DNA must be fragmented by reverse transcriptase

A) DNA strands must be homologous, having regions with similar or identical nucleotide sequences

2-D gel electrophoresis may be used to isolate specific proteins for purification and additional study. A) True B) False

A) True

In situ hybridization is used to detect the physical location of a gene on a chromosome. A) True B) False

A) True

The position of the bound radioactive probe is made visible on x-ray film. A) True B) False

A) True

The second phase of 2-D gel electrophoresis separates proteins based on their_______. A) mass B) length C) isoelectric point D) density

A) mass

In hybridization A) two DNAs from the same source combine B) DNA from two separate sources combine C) DNA combines with complementary RNA D) DNA is split into two separate pieces

B) DNA from two separate sources combine

At high temperature, two complementary DNA strands will anneal. A) True B) False

B) False

FISH probes are fluorescently-labeled and designed to bind only to non-complementary DNA. A) True B) False

B) False

In the tube gel, each band represents a single type of protein. A) True B) False

B) False

Non-homologous DNA will attach to a DNA probe. A) True B) False

B) False

The DNA probe will bind to identical nucleotide sequences on the test sample. A) True B) False

B) False

The cells used in the FISH technique are first arrested during A) interphase. B) metaphase. C) telophase. D) prophase. E) anaphase.

B) metaphase.

At the end of a 2-D gel electrophoresis, proteins with a high mass would be found where? A) along the left edge of the gel B) along the right edge of the gel C) along the top edge of the gel D) along the bottom edge of the gel

C) along the top edge of the gel

The first phase of 2-D gel electrophoresis separates proteins based on their_______. A) mass B) length C) isoelectric point D) density E) none of the above

C) isoelectric point

The smaller the DNA fragment A) the closer to the origin it will appear B) the brighter color it produces with ethidium bromide C) the faster it migrates during separation by electrophoresis D) the slower it migrates during separation by electrophoresis

C) the faster it migrates during separation by electrophoresis

The purpose of the Southern Blot test is to A) look for a specific nucleotide sequence in the DNA being tested B) to determine how closely two organisms are related C) to identify the size of the fragment that contains the sequence D) a and c E) a, b, and c

D) a and c

DNA probes are used to A) locate complementary RNA sequences in a test sample B) make homologous DNA C) make homologous RNA D) locate a specific DNA nucleotide sequence in a test sample

D) locate a specific DNA nucleotide sequence in a test sample

Arrange the following in the proper sequence in which they occur during FISH. 1. Denaturation of DNA 2. Addition of DNA probes 3. Fixation of chromosomes A) 1, 2, 3 B) 2, 1, 3 C) 2, 3, 1 D) 3, 2, 1 E) 3, 1, 2

E) 3, 1, 2

The term in situ is ________ for ________ . A) Greek ; in cell B) Greek ; in place C) Greek ; in lab D) Latin ; in cell E) Latin ; in place

E) Latin ; in place