Chapter 8-Recombinant DNA Technology
Which of the following is NOT a goal of recombinant DNA technology?
making it possible to clone humans
Mutagens are useful in biotechnology research for
producing organisms with altered phenotypes.
Why would a recombinant DNA molecule be inserted into a host cell?
It can be copied, transcribed, and translated into a desired protein.
How do restriction enzymes cut DNA sequences?
They cut DNA at sites, called recognition sites, that have specific nucleotide sequences.
Put the following events in the construction of a recombinant DNA molecule in the correct order: a. ligation of desired gene to plasmid DNA b. introduction of the plasmid into bacteria c. restriction enzymes cut gene of interest and plasmid DNA d. growth of cells on an antibiotic-containing medium
c, a, b, d
Which of the following attaches the target gene to a desired location?
DNA ligase
In general, how might recombinant DNA technology be used to prevent a genetic disorder caused by a mutation in a single gene?
To insert a desirable gene, remove an undesirable gene, or replace a defective gene with a functioning gene
Which of the following enzymes can make specific cuts in DNA molecules?
restriction enzymes
