Endospore Staining

Please select all of the statements that are true about endospores. -Bacterial endospores are highly resistant to heat, drying and radiation. Endospores are only found in the environment and are not medically relevant. -Endospores can exist in the environment for indefinite periods of time. -Endospores resist boiling and therefore steam must be used to destroy endospores present in food. Endospores can be formed by any genus of bacteria.

-Bacterial endospores are highly resistant to heat, drying and radiation. -Endospores can exist in the environment for indefinite periods of time. -Endospores resist boiling and therefore steam must be used to destroy endospores present in food.

What is an endospore? A dormant structure formed by bacteria in response to nutrient depletion A dormant structure used by bacteria in response to higher temperatures A vegetative structure with a lowered metabolic rate A reproductive structure used by bacteria for population growth

A dormant structure formed by bacteria in response to nutrient depletion

If steam is not applied during the malachite green step, what is a likely result at the end of the staining procedure? Only sporangium will be green. Both spores and sporangium may be green. Both spores and sporangium may be clear. Both spores and sporangium may be pink.

Both spores and sporangium may be pink.

Which genera represent endospore-forming bacteria? Bacillus and Neisseria Clostridium and Staphylococcus Clostridium and Bacillus Bacillus and Streptococcus

Clostridium and Bacillus

What is the role of water in this staining method? Decolorizer Mordant Secondary stain

Decolorizer

Why is endospore staining important? It helps in identifying the few medically important genera that produces endospores. It helps in identifying the antibiotic susceptibility of a species. It separates bacteria from protozoans under the microscope. It demonstrates the reproductive capacity of a genus.

It helps in identifying the few medically important genera that produces endospores.

What is the primary stain used in the Schaeffer-Fulton staining method? Methylene blue Safranin Carbolfuchsin Malachite green

Malachite green

What is safranin used for in this staining method? Staining the vegetative cells Staining the endospores Used as a mordant

Staining the vegetative cells

What is used as a mordant in this endospore staining method? Safranin Water Malachite green Steam

Steam

Endospore staining is a good example of a _______ stain technique. fluorescent simple differential negative

differential

The final result of a Schaeffer-Fulton stained slide is clear spores and pink sporangium. pink spores and green sporangium. green spores and purple sporangium. green spores and pink sporangium.

green spores and pink sporangium

Move the descriptions into the correct boxes to assess your understanding of the differential staining techniques, including Gram stain, acid-fast stain, and spore stain.

spore stain- use malachite green distinguish between metabolically active cells and dormant structures gram stain- differentiates cells based on thickness of peptidoglycan layer, result in purple and pink cells , a chemical is used as a mordant acid fast stain- does not require heat to be used in the staining process, differentiates cells with high lipid content in cell wall, important diagnostic tool in mycobacterium infections, uses basic fuchsin, acid-alcohol, and methylene blue

Move the descriptions into the correct boxes to assess your understanding of the differences between endospores and vegetative cells.

vegetative cells: stained with safranin, actively metabolizing, high water content endospores: highly resistant to heat and chemicals, stain with malachite green require autoclave for destruction formed when conditions are unfavorable heat is the mordant to facilitate staining of these