enzyme lab answers for bio lab

what is bacillus subtilis

a common decay organism

what was the effect of cu2+ on enzyme activity? why did this occur?

copper is a non-competitive inhibitor of amylase. it is a non-competitive inhibitor because it does not look similar to the substrate. therefore, it is not competing with the substrate for the active site of the enzyme like a competitive inhibitor does. instead, it reduces ezyme activity in the copper treated test tube is slower than the control test tube containing only distilled water, buffer, enzyme, and starch

why is a human enzyme optimal temperature 37c

its 37c because of natural selection would favor those individuals with enzymes folded in the optimal three-dimensional configuration

what would you expect for amylase isolated from pugs and bacteria compared to humans

oysters, pigs, and many bacteria have different normal temperatures than humans; so, they would have different different optimal temperatures due to natural selection.

describe the enzyme activity observed at 0c. describe the enzyme activity at 100c. explain what is happening to the enzyme at both temperatures.

the 0c temperature should have had only a small amount of enzyme activity because the low temperature reduces the kinetic energy of the starch molecules. the enzyme was much lower because few of the collisions between the starch molecule and the active site of the enzyme will have sufficient activation energy to allow the reaction to pass. the 100c temp should have had no enzyme activity. at 100c the enzyme is denatured and loses its active state

what is what is the optimum temperature the amylase you tested and what kind of organism was involved. and why was it that temp

the amylase we used in lab was isolated from bacillus subtilis. since compost pile gets as warm as 70c, this organism has evolved to grow at warmer temperatures. the observer optimum temp should have been 70c

what was the optimum ph of amylase.

the experimental results of most studies indicated a ph optimum of either 6.0 or 7.0

what is the typical intracellular ph of cells

the intracellular ph of most organism is between 6 and 7. the enzyme optimum should have been either 6.0 or 7.0

what temperature would you expect to the optimum for a human salivary amylase.

the optimal temperature for most human enzymes should be the same as normal body temperature: 37 degrees celsius.

did high enzyme concentration increase the reaction rate? explain why.

yes, it takes less time for a larger number of enzyme molecules to hydrolyze a fixed number of substrate molecules.