Lab 3 - Analytical Techniques

100μL is equivalent to:

.1ml

The linear relationship between the concentration of a colored solution and its light absorbance is known as the

Beer-Lambert Law

What color-producing chemical will be used in today's lab to determine the concentration of glucose solutions through absorbance readings?

Chromogen

The color of a solution and the wavelength of light it absorbs have a(n) __________ relationship.

Inverse

What micropipette(s) could be used to measure out 175μL of liquid?

P200 or P1000

What is the name of the machine we will use in today's lab to measure the amount of light that is absorbed by a solution?

Spectrophotometer

how to determine if someone is a diabetic

The diagnosis for diabetes mellitus includes two fasting plasma glucose levels of 126 mg/dL or higher or two random glucose readings of 200 mg/dL or higher

If the results of a patient's two "random" glucose readings are 196 mg/dL and 200mg/dL, this person meets the criteria of diabetes mellitus.

True

Precision

measurements are reproducible. This means that if you repeat the measurement several times the actual volume will be the same each time

Blue-colored objects absorb which wavelength of light the most?

red

Accuracy

refers to the closeness of a measured value to a known value

standard curve

the linear relationship between the glucose concentration and the intensity of color produced by the chemical reaction.

Transmittance

the measure of how much light gets through a sample

Absorbance

the quantity of light absorbed by a solution