Lab Practical
seromicroscope
(dissecting scope or binocular scope) has two oculars.
one nano meter is equal to
1 billion of a meter
one micro liter is equal to
1 million of a lieter
at medium power, the diameter of the field view is
1.5 mm
under conditions of standard temperature and pressure, 1 liter of water would have a mass of
1000 g
if the objective lens is 10x, what is the total magnification of the microscope?
100x
the magnifying ocular lens is
10x
at low power the diameter of the field view is
3.5 mm
How is a "prepared slide" different from a "wet mount'?
A prepared slide is made in advance and lasts longer if taken care of and a wet mount is made during the experiment and is only temporary
CHEMICAL ASPECTS OF LIFE
CHEMICAL ASPECTS OF LIFE
standard unit of volume is
L
MICROSCOPE
MICROSCOPE
what is a wet mount?
a temporary slide preparation that uses water as mounting medium
control variables
all other variables that must remain constant during the test
hypotheisis
an educated guess to a scientific question
base
bears the weight of microscope
coarse adjustment
changes the distance between the slide and the objective to focus an image
field of view
circular field you see when you look through the ocular
objectives
contains lenses of various magnification
an accepted hypothesis
could later be show to be false
c.s
cross section
Does the light intensity increase or decrease as the magnification increases?
decreases
Does the working distance increase or decrease as the magnification increases?
decreases
which microscope(s) do (does) not use a beam of light?
electronic microscope
ORGANISM B -not submicroscopic -organism is not green -organism is unicellular (single cell). -flagella are present. -the falgella are long and whip-like.
flagellate
pointer
found in ocular, can be moved by turning the black eyepiece
Advantage over light microscope: scanning electron microscope
greater depth of focus, plus a higher magnification
ORGANISM A -not submicroscopic (you can see it using microscope) -has green color -color is not blue-green -pigment (color) is a leaf-green. -organism is not moving and no flagella can be seen -cells are arranged in a chain
green algae q
at what magnification is the field of view the smallest?
high
Advantage over light microscope: transmission electron microscope
higher magnification
stage clips
hold slide steady
experiment
is the step in acquiring knowledge that involves careful testing in all possible answers to a question from the natural world.
body tube
keeps ocular and objective lenses at proper distance from each other
standard unit of mass is
kg
ocular
lens nearest
l.s
longitudinal section
at what magnification can you view the largest areas on the slide?
low
standard unit of length is
m
ORGANISM C -cells are not submicroscopic -organism is not green -is not unicellular and appears to be multi-celled. -body shape is worm-like. -body is unsegmented (no sections or division visible, smooth outer exterior); moves in a whip-like fashion
nematode
water tends to cling to glass surfaces but not to plastic. If you place water in a plastic graduated cylinder, would it have a meniscus?
no
fine adjustment
permits exact focusing
nosepiece
permits interchange of low, medium, and high power objectives
What does the term "opaque" mean?
preventing transmission of light
illuminator
provides light source
iris diaphragm
regulates amount of light going through specimen
to be valid, all scientific experiments
should be repeated with the same result
null hypothesis
states that the treatment has no effect on the outcome of the experiment
which of the four types of microscopes discussed does not invert the image?
steroscope
arm
supports body tube and adjustment knobs
stage
supports slides
magnification
tells how much larger the object appears under the scope than it actually is
Describe the "field of view"
the circular field that you see when you look through the ocular
what is meant by the working distance of the microscope?
the distance between the objective and the slide
experimental group
the group that being tested on a receive's the independent variable and is expected to change and compare to the control group for results
control group
the group that is being compared to the experimental group it does not receive the independent variable
dependent variable
the observed (measured or counted) outcome of the test; depends on the outcome of what is being manipulated in the "independent variable".
depth of focus
the thickness of an object that is all in sharp focus at the same time.
meniscus
the upward or downward curve at the surface of a liquid in a container occurs because of surface tension
independent variable
the variable that doesn't depend on the another action to change
Advantage over light microscope: Phase-contrast microscope
tiny objects such as cilia and flagella can be seen clearly
what is the function of the pointer on the microscope?
to indicate specific structures or regions of objects being examined
w.m
whole mount
How does the following change when you go from low power to high power magnification? working distance field of view light intesnity
working distance: becomes less field of view: shrinks (becomes smaller) light intensity: becomes less