Lab Practical

seromicroscope

(dissecting scope or binocular scope) has two oculars.

one nano meter is equal to

1 billion of a meter

one micro liter is equal to

1 million of a lieter

at medium power, the diameter of the field view is

1.5 mm

under conditions of standard temperature and pressure, 1 liter of water would have a mass of

1000 g

if the objective lens is 10x, what is the total magnification of the microscope?

100x

the magnifying ocular lens is

10x

at low power the diameter of the field view is

3.5 mm

How is a "prepared slide" different from a "wet mount'?

A prepared slide is made in advance and lasts longer if taken care of and a wet mount is made during the experiment and is only temporary

CHEMICAL ASPECTS OF LIFE

CHEMICAL ASPECTS OF LIFE

standard unit of volume is

L

MICROSCOPE

MICROSCOPE

what is a wet mount?

a temporary slide preparation that uses water as mounting medium

control variables

all other variables that must remain constant during the test

hypotheisis

an educated guess to a scientific question

base

bears the weight of microscope

coarse adjustment

changes the distance between the slide and the objective to focus an image

field of view

circular field you see when you look through the ocular

objectives

contains lenses of various magnification

an accepted hypothesis

could later be show to be false

c.s

cross section

Does the light intensity increase or decrease as the magnification increases?

decreases

Does the working distance increase or decrease as the magnification increases?

decreases

which microscope(s) do (does) not use a beam of light?

electronic microscope

ORGANISM B -not submicroscopic -organism is not green -organism is unicellular (single cell). -flagella are present. -the falgella are long and whip-like.

flagellate

pointer

found in ocular, can be moved by turning the black eyepiece

Advantage over light microscope: scanning electron microscope

greater depth of focus, plus a higher magnification

ORGANISM A -not submicroscopic (you can see it using microscope) -has green color -color is not blue-green -pigment (color) is a leaf-green. -organism is not moving and no flagella can be seen -cells are arranged in a chain

green algae q

at what magnification is the field of view the smallest?

high

Advantage over light microscope: transmission electron microscope

higher magnification

stage clips

hold slide steady

experiment

is the step in acquiring knowledge that involves careful testing in all possible answers to a question from the natural world.

body tube

keeps ocular and objective lenses at proper distance from each other

standard unit of mass is

kg

ocular

lens nearest

l.s

longitudinal section

at what magnification can you view the largest areas on the slide?

low

standard unit of length is

m

ORGANISM C -cells are not submicroscopic -organism is not green -is not unicellular and appears to be multi-celled. -body shape is worm-like. -body is unsegmented (no sections or division visible, smooth outer exterior); moves in a whip-like fashion

nematode

water tends to cling to glass surfaces but not to plastic. If you place water in a plastic graduated cylinder, would it have a meniscus?

no

fine adjustment

permits exact focusing

nosepiece

permits interchange of low, medium, and high power objectives

What does the term "opaque" mean?

preventing transmission of light

illuminator

provides light source

iris diaphragm

regulates amount of light going through specimen

to be valid, all scientific experiments

should be repeated with the same result

null hypothesis

states that the treatment has no effect on the outcome of the experiment

which of the four types of microscopes discussed does not invert the image?

steroscope

arm

supports body tube and adjustment knobs

stage

supports slides

magnification

tells how much larger the object appears under the scope than it actually is

Describe the "field of view"

the circular field that you see when you look through the ocular

what is meant by the working distance of the microscope?

the distance between the objective and the slide

experimental group

the group that being tested on a receive's the independent variable and is expected to change and compare to the control group for results

control group

the group that is being compared to the experimental group it does not receive the independent variable

dependent variable

the observed (measured or counted) outcome of the test; depends on the outcome of what is being manipulated in the "independent variable".

depth of focus

the thickness of an object that is all in sharp focus at the same time.

meniscus

the upward or downward curve at the surface of a liquid in a container occurs because of surface tension

independent variable

the variable that doesn't depend on the another action to change

Advantage over light microscope: Phase-contrast microscope

tiny objects such as cilia and flagella can be seen clearly

what is the function of the pointer on the microscope?

to indicate specific structures or regions of objects being examined

w.m

whole mount

How does the following change when you go from low power to high power magnification? working distance field of view light intesnity

working distance: becomes less field of view: shrinks (becomes smaller) light intensity: becomes less