LabCE Course - Blood Cell ID
CD19 CD20 Kappa or Lambda
Select each marker listed below that is expressed by a normal mature B cell
Lymphocytes
What cell population is gated in this peripheral blood sample case analyzed by flow cytometry
Gated
When analyzing the raw data of a sample on the flow cytometer, CD marker information on the histograms represents data inside the _____ population
T-helper cells
Which of the following types of lymphocytes express CD4
Granulocytes
Which of these white blood cell populations would have the MOST side scatter when analyzed using flow cytometry
Chronic lymphocytic leukemia
A monoclonal B-cell population (Kappa or Lambda predominant) with expression of CD19, CD20, CD23, and co-expression of CD5 is consistent with which of the following
Cells in the gated population are coexpressing CD5 and CD20
A population of small lymphocytes is gated for further evaluation in the flow cytometry laboratory. The scatterplot for one of the evaluations is shown on the right. How would you interpret this scatterplot
Mature T Cells
CD5 antigen is normally found on which of the following lymphocyte populations
True
All helper cells are T cells. Consequently, in a normal lymphocyte population, it is reasonable to expect all CD4-positive cells to be CD3-positive as well. True or False
True
Cell size is indicated by forward scatter and granularity is indicated by side scatter in flow cytometry
Lysed
During the 'staining' process prior to flow cytometry analysis of white blood cells, erythrocytes are _______
Intrinsic cell characteristics Extrinsic cell characteristics Forward scatter Fluorescence
During the flow cytometry analysis process, cells exiting the flow cell intersect a laser, and produce what kind of data
False
Extrinsic cell characteristics are reflected by the forward and side scatter on a flow cytometry cytogram
93%
If 100% of the cells in the gated lymphocyte population are positive for CD45 (i.e., they are leukocytes) and 7% are B cells (represented by CD19 and CD20 positivity), approximately what percentage of T cells would be expected
B cells
It has already been established that the gated cell population is lymphocytic in nature. CD19 and CD20 cell surface antigens both appear on what type of lymphocytes
False
Monoclonal antibodies are designed to bind to all antigens on cell surfaces
Leukocytes
The image on the right contains the fluorescence data from CD45 from a flow cytometry analysis. Note that virtually all cells in the gated population have CD45 surface antigens, indicating they are which type of cells
CD 20
Which of the following CD markers is NOT present on a normal mature T cell