LabCE Course - Blood Cell ID

CD19 CD20 Kappa or Lambda

Select each marker listed below that is expressed by a normal mature B cell

Lymphocytes

What cell population is gated in this peripheral blood sample case analyzed by flow cytometry

Gated

When analyzing the raw data of a sample on the flow cytometer, CD marker information on the histograms represents data inside the _____ population

T-helper cells

Which of the following types of lymphocytes express CD4

Granulocytes

Which of these white blood cell populations would have the MOST side scatter when analyzed using flow cytometry

Chronic lymphocytic leukemia

A monoclonal B-cell population (Kappa or Lambda predominant) with expression of CD19, CD20, CD23, and co-expression of CD5 is consistent with which of the following

Cells in the gated population are coexpressing CD5 and CD20

A population of small lymphocytes is gated for further evaluation in the flow cytometry laboratory. The scatterplot for one of the evaluations is shown on the right. How would you interpret this scatterplot

Mature T Cells

CD5 antigen is normally found on which of the following lymphocyte populations

True

All helper cells are T cells. Consequently, in a normal lymphocyte population, it is reasonable to expect all CD4-positive cells to be CD3-positive as well. True or False

True

Cell size is indicated by forward scatter and granularity is indicated by side scatter in flow cytometry

Lysed

During the 'staining' process prior to flow cytometry analysis of white blood cells, erythrocytes are _______

Intrinsic cell characteristics Extrinsic cell characteristics Forward scatter Fluorescence

During the flow cytometry analysis process, cells exiting the flow cell intersect a laser, and produce what kind of data

False

Extrinsic cell characteristics are reflected by the forward and side scatter on a flow cytometry cytogram

93%

If 100% of the cells in the gated lymphocyte population are positive for CD45 (i.e., they are leukocytes) and 7% are B cells (represented by CD19 and CD20 positivity), approximately what percentage of T cells would be expected

B cells

It has already been established that the gated cell population is lymphocytic in nature. CD19 and CD20 cell surface antigens both appear on what type of lymphocytes

False

Monoclonal antibodies are designed to bind to all antigens on cell surfaces

Leukocytes

The image on the right contains the fluorescence data from CD45 from a flow cytometry analysis. Note that virtually all cells in the gated population have CD45 surface antigens, indicating they are which type of cells

CD 20

Which of the following CD markers is NOT present on a normal mature T cell