Light Microscope Lab

Arm/frame

Supports the tube and connects to the base

Light source

brightens up the field of view for better visualization of specimen under examination

Coarse adjustment knob

drastic movement of stage to bring the object in the slide into focus. For use with scanning (4X) objective lens

Mechanical stage knob

moves the stage in different directions

Stage Clip

securers the slide in place on the stage

Stage

supports slides or specimen to be viewed

Base

the bottom of the microscope, used for support

Condenser

Directs light onto the specimen

What knob is used when viewing specimen under high power and oil-immersion?

Fine adjustment knob

Observation Tube

Connects the eyepiece to the objective lenses

What does the microscope do to the image?

Invert and Flip

Ocular Lens

Lenses an observer looks into. They have a magnifying power of 10X

Fine adjustment knob

Slight movement of stage to bring the object in the slide into focus. For use with low power (10X), high power (40X), and oil-immersion (100X) objective lens

Objective lens

4 lenses that carry different powers of magnification (4X, 10X, 40X, and 100X) on their own

If ocular lens is 10X and the objective lens is 40X, what is the total magnification?

400X

What is the total magnification of high power (blue)?

400X

What is the total magnification of scanning (red)?

40X

Revolving Nosepiece

An apparatus that holds the objective lenses and use to change between the different objective lenses.

If the ocular lens of a microscope is 5X and the objective lens is 20X, what is the total magnification?

100X

What is the total magnification of low power (yellow)?

100X

What is the best objective lens to view bacteria?

Oil-immersion (1000X, white)

Main Switch

Power on/off switch

Iris diaphragm and iris diaphragm control

Vary the amount of light projected onto the slide