Micro Test 3 Practice Questions

in fermentation an end product cannot be

pyruvate because that is what is being oxidized

What is the end product of glycolysis? A: alcohol B: lactic acid C:pyruvic acid D: acetyl CoA

pyruvic acid

in the step of glycolysis NAD has undergone

reductions

staphylococcus Arueas is capable of growth on a nutrient agar containing 10% salt. this medium is described as a selective differential undefined chemically defined both selective and differential

selective and differential

Two cells with a generation time of 20 minutes grew over 2 hours to a colony of cells. How many cells were in the colony? A:32 cells B: 128 cells C: 256 cells D: 64 cells

B

the agar is added to the culture media to serve as a solidifying agent reducing agent carbohydrate agent protein source growth factor

solidifying agent

in the reaction shown below ATP is formed via A: Chemiosmosis B: Photophosphorylation C: substrate phosphorylation D: oxidation- reduction

substrate phosphorylation

in the diagram shown below the pink arrow represents A: activation energy with an enzyme B: activation energy without enzyme C: the energy level of the products

the activation energy with enzyme

where does the ATP get made in the mitochondrion?

the matrix

how are aerobic respiration and anaerobic respiration different? Anaerobic respiration a: doesnt use chemiosmosis b: uses an organic electron acceptor c: uses an inorganic electron acceptors such as nitrates or carbonate d: produces more ATP per glucose molecule than aerobic respiration

uses inorganic electron acceptors such as sulfate, nitrate, or carbonate

what does glycolysis produce

2 pyruvate 2 ATP 2 NADH

a culture is inoculated with 2 cells. How many cells are in the culture after 6 generations A:60 b:64 c:320 D:640

2^6=64

34 colonies grow on a pour plate made with 1 ml from the 3rd tube in a 1:10 dilution series. What is the population of the original sample 34 340 3400 24000

3400

these graphs indicate optimum enzyme activity will occur at

37: C and pH:5

Which of the following is most likely to be killed by atmospheric oxygen? A: obligate anaerobe B: aerotolerant anaerobe C: obligate aerobe D: facultative anaerobe

B: Obligate anaerobe

The most Carbon Dioxide is going to be produced in the Electron Transport Chain as a waste product of the breakdown of glucose? T/F

F

The main pathway(s) of cell respiration in metabolism is/are ____________________________. A: Glycolysis, Krebs (TCA) cycle, Electron Transport Chain (pentose phosphate pathway) B: glycolysis only C: Krebs (TCA) cycle and Electron transport chain (pentose phosphate pathway) D: glycolysis and the Krebs Cycle (TCA cycle)

Glycolysis, Krebs (TCA) cycle, Electron Transport Chain (pentose phosphate pathway)

Mr Big wanted to make wine for his upcoming festival but instead his wine ended up as vinegar. What happened to Big's wine? A: He fermented grapes but forgot to keep holes in the lid so the organisms would not die because they had enough air (oxygen). B: He fermented grapes but spoilage organisms turned wine into vinegar. C: He forgot to use grapes. D: He fermented grapes but yeast got into his wine and turned it into vinegar.

He fermented grapes but spoilage organisms turned wine into vinegar.

When a bacterial cell is placed in a solution with a higher solute concentration relative to it's cellular content, this would be an example of ____________________, and the cell would _______________________. A:hypertonic; plasmolyze B: hypertonic; lyse C: hypotonic; lyse D: hypotonic; plasmolyze

Hypertonic plasmolyze

Cells would be most sensitive to penicillin during which phase of growth? A: log phase B: stationary phase C: lag phase D: prolonged decline

Log phase

which of these is the oxidized form the electron carrier and thus has less energy A:NADPH B:NADH C:NAD+ D:O2

NAD+

which of these temperatures is most likely to kill a mesophilic bacteria below 0 c 15c 37c 45c above 60c

above 60c

which of these temperatures is where you would most likely to find hyperthermophilic prokaryotes below 0 c 15c 37c 65c above 90 c

above 90

the region of an enzymes that binds with substrate molecules is the A: coenzyme B: holoenzyme C: active site D: allosteric site e: Feedback site

active site

which of these terms describes microbes that do not use oxygen but can grow if it is present in small amounts A:obligate aerobe B: obligate anaerobe C:faculative anaerobe D: microaerophile E: aerotolerant anaerobes

aerotolerant anaerobe

the enzyme shown in the previous diagram is

all of the above

which of these is an endergonic reaction A: anabolic B: are also biosynthetic reactions C: breakdown of polymers into monomers D:catabolic E: A and B

anabolic

which enzyme converts hydrogen peroxide to water and oxygen peroxidase catalase superoxide dismutase all of the above

catalase

sugars and salts are used to preserve foods because they induce the formation of oxygen free radicals inhibit cell wall biosynthesis cause cell lysis by osmotic shock cause plasmolysis of cells change the ph of the food

cause plasmolysis of cells

the nutrient broth that grows most microorganisms is commonly used in microbiology labs and is an example of selective media enrichment media complex media chemically defined media differential media

complex media

the type of media that promotes the growth of fastidious bacteria is an example of selective media enrichment media complex media chemically defined media differential media

complex media

ATP gets made in the

cytosol of the prokaryotes

blood agar is an example of ______media A: enrichment B: selective C: chemically defined D: differential E: A and D

differential

the advantage of direct counting method over the serial plate method is that direct counting counts both live and dead cells counts only living cells does not not require incubation can be used with low numbers of cells

does not require incubation

which of these terms describes microbes that prefer oxygen but can grow if it is not present. They can grow with or without oxygen A: Obligate aerobe b: Obligate anaerobe c: facultative anaerobe D: microaerophiles E:aerotolerant anaerobe

facultative anaerobe

most marine microorganisms are A: Hyperthermophiles B: Acidophiles C:Psychrophiles D:Facultative Halophiles E: Obligate halophiles

facultative halophiles

the name of the first pathway is

glycolysis

trace elements include elements such as zinc iron and copper are used as enzyme cofactors are frequently added in high concentration in culture media A and B All of the above

include elements such as iron, copper and zinc and cofactors

in the previous slide the pathway labeled #2 is the

kreb cycle

Which growth phase is also called the exponential growth phase A: lAG b: Log c: stationary d:decline phase

log phase

which of these terms describe a microorganism that grows at human body temp of 37c A:Thermophile B: Psychophile C:Psychtroph D: Mesophile E:Hyperthermophile

mesophile

in eukaryotes the electron transport chains is located in the

mitochochorion

organisms such as Clostridium lack oxygen detoxifying enzymes thus they are obligate aerobes obligate anaerobes faculattive anaerobes microaerophiles aerotolerant anaerobes

obligate anaerobe

Which of these give the cells the ability to produce the most ATP? A: substrate level phosphorylation B: reductive phosphorylation C: photophosphorylation D: oxidative phosphorylation

oxidative phosphorylation

all bacteria require the following for growth except oxygen nitrogen phosphorus sulfur carbon

oxygen

which of the following are direct measurements of bacterial population

petrof hauser

A moleculeof ADP that accepts a phosphate becomes __________________ A: carboxylated B: phosphorylated C: oxidized D:reduced

phosphorylated

in prokaryotes in electron transport chain is located in the

plasma membrane

the pathway is shutdown by high concentrations of

product

Catabolic reactions A: provide building blocks for anabolism B: are also biosynthetic reactions C: Are endergonic D: include the formation of proteins from amino acids

provide the buliding blocks for anabolism

which of these describes a microorganism that grows in a refrigerator temperature and can cause food spoilage of 15c thermophile psychrophile psychrotroph mesophile hyperthermophile

psychrotroph